Role of autophagy in apoptosis induction by methylene chloride extracts of Mori cortex in NCI-H460 human lung carcinoma cells

  • Authors:
    • Shin-Hyung Park
    • Gyoo Yong Chi
    • Hyun Sup Eom
    • Gi-Young Kim
    • Jin  Won Hyun
    • Wun-Jae Kim
    • Su-Jae Lee
    • Young Hyun Yoo
    • Yung Hyun Choi
  • View Affiliations

  • Published online on: February 22, 2012     https://doi.org/10.3892/ijo.2012.1386
  • Pages: 1929-1940
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Abstract

The root of Mori cortex has traditionally been used in Korea for the treatment of cutaneous inflammation, pulmonary asthma, and congestion for thousands of years. The present study was designed to validate the anticancer effects of methylene chloride extracts of the M. cortex root (MEMC) in NCI-H460 human lung carcinoma cells. Exposure to MEMC was found to result in growth inhibition by the induction of caspase‑dependent apoptosis in NCI-H460 cells, which correlated with upregulated expression of death receptor (DR)4, DR5 and FasL, downregulation of anti-apoptotic Bcl-2 and Bcl-xL expression, cleavage of Bid, and loss of mitochondrial membrane potential. In addition, autophagosomes, a characteristic finding of autophagy, and markers of autophagy, conversion of microtubule-associated protein light chain-3 (LC3)-I to LC3-II and increased beclin-1 accumulation, were observed in MEMC-treated NCI-H460 cells. Inhibition of autophagy by 3-methyladenine or LC3B small interfering (siRNA) resulted in enhanced apoptotic cell death, suggesting that MEMC-induced autophagy functions as a suppressor of apoptosis. MEMC-induced autophagy was also blocked by N-acetyl-cysteine (NAC) and catalase, indicating that H2O2 can regulate autophagy. Our data demonstrate that MEMC triggers both ROS-mediated autophagy and caspase-dependent apoptosis, and that autophagy plays a protective role against apoptotic cell death.

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June 2012
Volume 40 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park S, Chi GY, Eom HS, Kim G, Hyun JW, Kim W, Lee S, Yoo YH and Choi YH: Role of autophagy in apoptosis induction by methylene chloride extracts of Mori cortex in NCI-H460 human lung carcinoma cells. Int J Oncol 40: 1929-1940, 2012
APA
Park, S., Chi, G.Y., Eom, H.S., Kim, G., Hyun, J.W., Kim, W. ... Choi, Y.H. (2012). Role of autophagy in apoptosis induction by methylene chloride extracts of Mori cortex in NCI-H460 human lung carcinoma cells. International Journal of Oncology, 40, 1929-1940. https://doi.org/10.3892/ijo.2012.1386
MLA
Park, S., Chi, G. Y., Eom, H. S., Kim, G., Hyun, J. W., Kim, W., Lee, S., Yoo, Y. H., Choi, Y. H."Role of autophagy in apoptosis induction by methylene chloride extracts of Mori cortex in NCI-H460 human lung carcinoma cells". International Journal of Oncology 40.6 (2012): 1929-1940.
Chicago
Park, S., Chi, G. Y., Eom, H. S., Kim, G., Hyun, J. W., Kim, W., Lee, S., Yoo, Y. H., Choi, Y. H."Role of autophagy in apoptosis induction by methylene chloride extracts of Mori cortex in NCI-H460 human lung carcinoma cells". International Journal of Oncology 40, no. 6 (2012): 1929-1940. https://doi.org/10.3892/ijo.2012.1386