Anti-HuC and -HuD autoantibodies are differential sero-diagnostic markers for small cell carcinoma from large cell neuroendocrine carcinoma of the lung

  • Authors:
    • Toshihide Matsumoto
    • Shinichiro Ryuge
    • Makoto Kobayashi
    • Taihei Kageyama
    • Manabu Hattori
    • Naoki Goshima
    • Shi-Xu Jiang
    • Makoto Saegusa
    • Akira Iyoda
    • Yukitoshi Satoh
    • Noriyuki Masuda
    • Yuichi Sato
  • View Affiliations

  • Published online on: March 16, 2012     https://doi.org/10.3892/ijo.2012.1405
  • Pages: 1957-1962
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Abstract

Aiming to identify novel sero-diagnostic markers for neuroendocrine carcinomas of the lung, the two-dimensional gel electrophoresis-immunoblot method was used to analyze tumor-associated autoantibodies in patients with small cell lung carcinoma (SCLC) and large cell neuroendocrine carcinoma (LCNEC). Several autoantigens were revealed and anti-HuC autoantibody was detected only in sera of SCLC patients. Since Hu family proteins including HuC are well-known causes of paraneoplastic encephalomyelitis/sensory neuronopathy (PEM/SN), the expression of HuC as well as HuD mRNAs and their proteins was studied in 11 lung cancer cell lines. The expression of HuC and HuD mRNAs and proteins was only detected in SCLC- and LCNEC-derived cells. To validate the existence of anti-HuC and -HuD auto-antibodies, we studied a large number of sera including those from lung cancer patients employing dot blot analysis. Anti-HuC and -HuD autoantibodies were detected only in SCLC cases with or without PEM/SN, and not in the sera of LCNEC patients. The mechanism leading to different anti-HuC and -HuD autoantibody production between SCLC and LCNEC is unclear; however, the results from the present and previous studies suggest that anti-HuC and -HuD auto-antibodies are novel differential sero-diagnostic markers for SCLC from LCNEC.

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June 2012
Volume 40 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Matsumoto T, Ryuge S, Kobayashi M, Kageyama T, Hattori M, Goshima N, Jiang S, Saegusa M, Iyoda A, Satoh Y, Satoh Y, et al: Anti-HuC and -HuD autoantibodies are differential sero-diagnostic markers for small cell carcinoma from large cell neuroendocrine carcinoma of the lung. Int J Oncol 40: 1957-1962, 2012
APA
Matsumoto, T., Ryuge, S., Kobayashi, M., Kageyama, T., Hattori, M., Goshima, N. ... Sato, Y. (2012). Anti-HuC and -HuD autoantibodies are differential sero-diagnostic markers for small cell carcinoma from large cell neuroendocrine carcinoma of the lung. International Journal of Oncology, 40, 1957-1962. https://doi.org/10.3892/ijo.2012.1405
MLA
Matsumoto, T., Ryuge, S., Kobayashi, M., Kageyama, T., Hattori, M., Goshima, N., Jiang, S., Saegusa, M., Iyoda, A., Satoh, Y., Masuda, N., Sato, Y."Anti-HuC and -HuD autoantibodies are differential sero-diagnostic markers for small cell carcinoma from large cell neuroendocrine carcinoma of the lung". International Journal of Oncology 40.6 (2012): 1957-1962.
Chicago
Matsumoto, T., Ryuge, S., Kobayashi, M., Kageyama, T., Hattori, M., Goshima, N., Jiang, S., Saegusa, M., Iyoda, A., Satoh, Y., Masuda, N., Sato, Y."Anti-HuC and -HuD autoantibodies are differential sero-diagnostic markers for small cell carcinoma from large cell neuroendocrine carcinoma of the lung". International Journal of Oncology 40, no. 6 (2012): 1957-1962. https://doi.org/10.3892/ijo.2012.1405