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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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August 2012 Volume 41 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Screening of significantly hypermethylated genes in breast cancer using microarray-based methylated-CpG island recovery assay and identification of their expression levels

  • Authors:
    • Zhen-Qiang Lian
    • Qi Wang
    • Wen-Ping Li
    • An-Qin Zhang
    • Ling  Wu
  • View Affiliations / Copyright

    Affiliations: Breast Disease Center, Guangdong Women and Children Hospital of Guangzhou Medical College, Guangzhou 510010, P.R. China
  • Pages: 629-638
    |
    Published online on: May 8, 2012
       https://doi.org/10.3892/ijo.2012.1464
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Abstract

To screen candidate methylation markers for early detection of breast cancer and to explore the relationship between methylation and gene expression, we performed methylated-CpG island recovery assay (MIRA) combined with CpG island array on 61982 CpG sites across 4162 genes in 10 cancerous and 10 non-cancerous breast tissues. Direct bisulfite sequencing and combined bisulfite restriction analysis (COBRA) were carried out in independent cancerous and non-cancerous samples. Gene expression was analyzed by microarrays and validated using RT-PCR. We detected 70 significantly hypermethylated genes in breast cancer tissues, including many novel hypermethylated genes such as ITGA4, NFIX, OTX2 and FGF12. Direct bisulfite sequencing showed widespread methylation occurring in intragenic regions of the WT1, PAX6 and ITGA4 genes and in the promoter region of the OTX2 gene in breast cancer tissues. COBRA assay confirmed that the WT1, OTX2 and PAX6 genes were hypermethylated in breast cancer tissues. Clustering analysis of the gene expression of 70 significantly hypermethylated genes revealed that most hypermethylated genes in breast cancer were not expressed in breast tissues. RT-PCR assay confirmed that WT1 and PITX2 were only weakly expressed in the breast cancer tissues and were not expressed in most non-cancerous breast tissues. OTX2 and PAX6 were not expressed in either breast cancer or non-cancerous tissues. In conclusion, these results will expand our knowledge of hypermethylated genes and methylation sites for early detection of breast cancer and deepen our understanding of the relationship between methylation and gene expression. The MIRA approach can screen candidate methylated genes for further clinical validation more effectively than gene expression microarray-based strategy.
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Copy and paste a formatted citation
Spandidos Publications style
Lian Z, Wang Q, Li W, Zhang A and Wu L: Screening of significantly hypermethylated genes in breast cancer using microarray-based methylated-CpG island recovery assay and identification of their expression levels. Int J Oncol 41: 629-638, 2012.
APA
Lian, Z., Wang, Q., Li, W., Zhang, A., & Wu, L. (2012). Screening of significantly hypermethylated genes in breast cancer using microarray-based methylated-CpG island recovery assay and identification of their expression levels. International Journal of Oncology, 41, 629-638. https://doi.org/10.3892/ijo.2012.1464
MLA
Lian, Z., Wang, Q., Li, W., Zhang, A., Wu, L."Screening of significantly hypermethylated genes in breast cancer using microarray-based methylated-CpG island recovery assay and identification of their expression levels". International Journal of Oncology 41.2 (2012): 629-638.
Chicago
Lian, Z., Wang, Q., Li, W., Zhang, A., Wu, L."Screening of significantly hypermethylated genes in breast cancer using microarray-based methylated-CpG island recovery assay and identification of their expression levels". International Journal of Oncology 41, no. 2 (2012): 629-638. https://doi.org/10.3892/ijo.2012.1464
Copy and paste a formatted citation
x
Spandidos Publications style
Lian Z, Wang Q, Li W, Zhang A and Wu L: Screening of significantly hypermethylated genes in breast cancer using microarray-based methylated-CpG island recovery assay and identification of their expression levels. Int J Oncol 41: 629-638, 2012.
APA
Lian, Z., Wang, Q., Li, W., Zhang, A., & Wu, L. (2012). Screening of significantly hypermethylated genes in breast cancer using microarray-based methylated-CpG island recovery assay and identification of their expression levels. International Journal of Oncology, 41, 629-638. https://doi.org/10.3892/ijo.2012.1464
MLA
Lian, Z., Wang, Q., Li, W., Zhang, A., Wu, L."Screening of significantly hypermethylated genes in breast cancer using microarray-based methylated-CpG island recovery assay and identification of their expression levels". International Journal of Oncology 41.2 (2012): 629-638.
Chicago
Lian, Z., Wang, Q., Li, W., Zhang, A., Wu, L."Screening of significantly hypermethylated genes in breast cancer using microarray-based methylated-CpG island recovery assay and identification of their expression levels". International Journal of Oncology 41, no. 2 (2012): 629-638. https://doi.org/10.3892/ijo.2012.1464
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