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Article

A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type

  • Authors:
    • Anna Otte
    • Gudrun Göhring
    • Doris Steinemann
    • Brigitte Schlegelberger
    • Stephanie Groos
    • Florian Länger
    • Hans-Heinrich Kreipe
    • Axel Schambach
    • Thomas Neumann
    • Peter Hillemanns
    • Tjoung-Won Park-Simon
    • Ralf Hass
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Medical University, Hannover, Germany, Institute of Cell and Molecular Pathology, Medical University, Hannover, Germany, Institute of Cell Biology, Medical University, Hannover, Germany, Institute of Pathology, Medical University, Hannover, Germany, Department of Experimental Hematology, Medical University, Hannover, Germany
  • Pages: 765-775
    |
    Published online on: May 8, 2012
       https://doi.org/10.3892/ijo.2012.1468
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Abstract

The small cell ovarian carcinoma of the hypercalcemic type (SCCOHT) represents an aggressive tumor with poor prognosis predominantly affecting young women and so far, no cell line or animal model is available to investigate this devastating disease. Biopsy material from a recurrent SCCOHT was subjected to an explant culture to obtain an adherent and continuously proliferating cell population. Morphological and functional characterization revealed a heterogeneous population (SCCOHT-1) of about 13 µm in diameter and approximately 36 h of doubling time. Flow cytometric analysis of surface markers demonstrated the expression of CD15, CD29, CD44 and CD90 paralleled by the presence of cytokeratins and vimentin. Cytogenetic analysis and high-resolution oligo-array comparative genomic hybridization (aCGH) demonstrated a stable karyotype including deletions of the PARK2, CSMD1, GRIN2B and ATF7IP genes. Following lentiviral transduction with a GFP vector, the labeled SCCOHT-derived cells were subjected to CCE to separate distinct subpopulations as evidenced by cell cycle analysis. Subcutaneous injection of these subpopulations into NOD/SCID mice exhibited hypercalcemia and a tumor development in 100% of the mice. Re-cultivation of the mouse tumors revealed an outgrowth of SCCOHT-derived phenotypes and all cell populations expressed high telomerase activity. Moreover, histopathological evaluation demonstrated close similarities between the mouse tumors and the original patient tumor. In conclusion, SCCOHT-1 cells provide a study platform to investigate this rare disease and to examine effective and sufficient therapeutic strategies for this rather unknown type of cancer.
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1 

Dickersin GR, Kline IW and Scully RE: Small cell carcinoma of the ovary with hypercalcemia: a report of eleven cases. Cancer. 49:188–197. 1982. View Article : Google Scholar : PubMed/NCBI

2 

Young RH, Oliva E and Scully RE: Small cell carcinoma of the hypercalcemic type in the ovary. Gynecol Oncol. 57:7–8. 1995.PubMed/NCBI

3 

Scully RE: Atlas of tumor pathology: tumors of the ovary and maldeveloped gonads. Armed Forces Institute of Pathology; Washington DC: 1979

4 

Eichhorn JH and Young RH: Transitional cell carcinoma of the ovary: a morphologic study of 100 cases with emphasis on differential diagnosis. Am J Surg Pathol. 28:453–463. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Geisinger KR, Kute TE, Pettenati MJ, et al: Characterization of a human ovarian carcinoma cell line with estrogen and progesterone receptors. Cancer. 63:280–288. 1989. View Article : Google Scholar

6 

Hamilton TC, Young RC, McKoy WM, et al: Characterization of a human ovarian carcinoma cell line (NIH:OVCAR-3) with androgen and estrogen receptors. Cancer Res. 43:5379–5389. 1983.PubMed/NCBI

7 

Poels LG, Jap PH, Ramaekers FF, et al: Characterization of a hormone-producing ovarian carcinoma cell line. Gynecol Oncol. 32:203–214. 1989. View Article : Google Scholar : PubMed/NCBI

8 

Bible KC, Boerner SA, Kirkland K, et al: Characterization of an ovarian carcinoma cell line resistant to cisplatin and flavopiridol. Clin Cancer Res. 6:661–670. 2000.PubMed/NCBI

9 

Ohi S, Niimi S, Okada N, et al: Establishment and characterization of a human ovarian small cell carcinoma, hypercalcemic type, cell line (OS-1) secreting PTH, PthrP and ACTH--special reference to the susceptibility of anti-cancer drugs. Hum Cell. 17:203–209. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Ulbright TM, Roth LM, Stehman FB, Talerman A and Senekjian EK: Poorly differentiated (small cell) carcinoma of the ovary in young women: evidence supporting a germ cell origin. Hum Pathol. 18:175–184. 1987. View Article : Google Scholar : PubMed/NCBI

11 

Aguirre P, Thor AD and Scully RE: Ovarian small cell carcinoma. Histogenetic considerations based on immunohistochemical and other findings. Am J Clin Pathol. 92:140–149. 1989.PubMed/NCBI

12 

Walt H, Hornung R, Fink D, et al: Hypercalcemic-type of small cell carcinoma of the ovary: characterization of a new tumor line. Anticancer Res. 21:3253–3259. 2001.PubMed/NCBI

13 

McCluggage WG, Oliva E, Connolly LE, McBride HA and Young RH: An immunohistochemical analysis of ovarian small cell carcinoma of hypercalcemic type. Int J Gynecol Pathol. 23:330–336. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Scully RE: Small cell carcinoma of hypercalcemic type. Int J Gynecol Pathol. 12:148–152. 1993. View Article : Google Scholar : PubMed/NCBI

15 

Eichhorn JH, Bell DA, Young RH, et al: DNA content and proliferative activity in ovarian small cell carcinomas of the hypercalcemic type. Implications for diagnosis, prognosis, and histogenesis. Am J Clin Pathol. 98:579–586. 1992.PubMed/NCBI

16 

Shrimali RK, Correa PD and Reed NS: Dose-dense and dose-intense chemotherapy for small cell ovarian cancer: 2 cases and review of literature. Med Oncol. 28:766–770. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Harrison ML, Hoskins P, du Bois A, et al: Small cell of the ovary, hypercalcemic type - analysis of combined experience and recommendation for management. A GCIG study. Gynecol Oncol. 100:233–238. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Benrubi GI, Pitel P and Lammert N: Small cell carcinoma of the ovary with hypercalcemia responsive to sequencing chemotherapy. South Med J. 86:247–248. 1993. View Article : Google Scholar : PubMed/NCBI

19 

Reed WC: Small cell carcinoma of the ovary with hypercalcemia: report of a case of survival without recurrence 5 years after surgery and chemotherapy. Gynecol Oncol. 56:452–455. 1995.PubMed/NCBI

20 

Barondeau J, Rodgers M, Braun L, Azarow K, Forouhar M and Faucette K: Small cell ovarian carcinoma: a rare, aggressive tumor masquerading as constipation in a teenager with a fatal outcome. J Pediatr Hematol Oncol. 32:e139–e141. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Dykgraaf RH, de Jong D, van Veen M, Ewing-Graham PC, Helmerhorst TJ and van der Burg ME: Clinical management of ovarian small-cell carcinoma of the hypercalcemic type: a proposal for conservative surgery in an advanced stage of disease. Int J Gynecol Cancer. 19:348–353. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Distelmaier F, Calaminus G, Harms D, et al: Ovarian small cell carcinoma of the hypercalcemic type in children and adolescents: a prognostically unfavorable but curable disease. Cancer. 107:2298–2306. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Gebert A and Preiss G: A simple method for the acquisition of high-quality digital images from analog scanning electron microscopes. J Microsc. 191:297–302. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Bertram C and Hass R: Cellular senescence of human mammary epithelial cells (HMEC) is associated with an altered MMP-7/HB-EGF signaling and increased formation of elastin-like structures. Mech Ageing Dev. 130:657–669. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Schlegelberger B, Metzke S, Harder S, Zühlke-Jenisch R, Zhang Y and Siebert R: Classical and Molecular Cytogenetics of Tumor Cells. Springer Verlag; Heidelberg: 1999

26 

Shaffer L, Slovak M and Champbell L: ISCN - An International System for Human Cytogenetic Nomenclature. S. Karger AG; Basel: 2009

27 

Gohring G, Hanke C, Kratz C, et al: Fluorescence in situ hybridization using the subtelomeric 11q probe as a diagnostic tool for congenital thrombocytopenia. Ann Hematol. 85:883–885. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Schambach A, Galla M, Modlich U, Will E, Chandra S, Reeves L, Colbert M, Williams DA, von Kalle C and Baum C: Lentiviral vectors pseudotyped with murine ecotropic envelope: increased biosafety and convenience in preclinical research. Exp Hematol. 34:588–592. 2006. View Article : Google Scholar : PubMed/NCBI

29 

McCluggage WG, McKenna M and McBride HA: CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors. Int J Gynecol Pathol. 26:322–327. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Ohishi Y, Kaku T, Oya M, Kobayashi H, Wake N and Tsuneyoshi M: CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls. Gynecol Oncol. 107:30–38. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Majore I, Moretti P, Hass R and Kasper C: Identification of subpopulations in mesenchymal stem cell-like cultures from human umbilical cord. Cell Commun Signal. 7:62009. View Article : Google Scholar : PubMed/NCBI

32 

Lavrentieva A, Majore I, Kasper C and Hass R: Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells. Cell Commun Signal. 8:182010. View Article : Google Scholar : PubMed/NCBI

33 

Pirozzi G, Tirion V, Camerlingo R, et al: Epithelial to mesenchymal transition by TGFbeta-1 induction increases stemness characteristics in primary non small cell lung cancer cell line. PLoS One. 6:e215482011. View Article : Google Scholar : PubMed/NCBI

34 

Martinez-Borges AR, Petty JK, Hurt G, Stribling JT, Press JZ and Castellino SM: Familial small cell carcinoma of the ovary. Pediatr Blood Cancer. 53:1334–1336. 2009. View Article : Google Scholar : PubMed/NCBI

35 

Denison SR, Callahan G, Becker NA, Phillips LA and Smith DI: Characterization of FRA6E and its potential role in autosomal recessive juvenile parkinsonism and ovarian cancer. Genes Chromosomes Cancer. 38:40–52. 2003. View Article : Google Scholar : PubMed/NCBI

36 

Kamal M, Shaaban AM, Zhang L, et al: Loss of CSMD1 expression is associated with high tumour grade and poor survival in invasive ductal breast carcinoma. Breast Cancer Res Treat. 121:555–563. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Park SY, Kwon HJ, Lee HE, et al: Promoter CpG island hyper-methylation during breast cancer progression. Virchows Arch. 458:73–84. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Turnbull C, Rapley EA, Seal S, et al: Variants near DMRT1, TERT and ATF7IP are associated with testicular germ cell cancer. Nat Genet. 42:604–607. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Otte A, Göhring G, Steinemann D, Schlegelberger B, Groos S, Länger F, Kreipe H, Schambach A, Neumann T, Hillemanns P, Hillemanns P, et al: A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type. Int J Oncol 41: 765-775, 2012.
APA
Otte, A., Göhring, G., Steinemann, D., Schlegelberger, B., Groos, S., Länger, F. ... Hass, R. (2012). A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type. International Journal of Oncology, 41, 765-775. https://doi.org/10.3892/ijo.2012.1468
MLA
Otte, A., Göhring, G., Steinemann, D., Schlegelberger, B., Groos, S., Länger, F., Kreipe, H., Schambach, A., Neumann, T., Hillemanns, P., Park-Simon, T., Hass, R."A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type". International Journal of Oncology 41.2 (2012): 765-775.
Chicago
Otte, A., Göhring, G., Steinemann, D., Schlegelberger, B., Groos, S., Länger, F., Kreipe, H., Schambach, A., Neumann, T., Hillemanns, P., Park-Simon, T., Hass, R."A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type". International Journal of Oncology 41, no. 2 (2012): 765-775. https://doi.org/10.3892/ijo.2012.1468
Copy and paste a formatted citation
x
Spandidos Publications style
Otte A, Göhring G, Steinemann D, Schlegelberger B, Groos S, Länger F, Kreipe H, Schambach A, Neumann T, Hillemanns P, Hillemanns P, et al: A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type. Int J Oncol 41: 765-775, 2012.
APA
Otte, A., Göhring, G., Steinemann, D., Schlegelberger, B., Groos, S., Länger, F. ... Hass, R. (2012). A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type. International Journal of Oncology, 41, 765-775. https://doi.org/10.3892/ijo.2012.1468
MLA
Otte, A., Göhring, G., Steinemann, D., Schlegelberger, B., Groos, S., Länger, F., Kreipe, H., Schambach, A., Neumann, T., Hillemanns, P., Park-Simon, T., Hass, R."A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type". International Journal of Oncology 41.2 (2012): 765-775.
Chicago
Otte, A., Göhring, G., Steinemann, D., Schlegelberger, B., Groos, S., Länger, F., Kreipe, H., Schambach, A., Neumann, T., Hillemanns, P., Park-Simon, T., Hass, R."A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type". International Journal of Oncology 41, no. 2 (2012): 765-775. https://doi.org/10.3892/ijo.2012.1468
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