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Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer

  • Authors:
    • Atsuki Sato
    • Toshiyuki Ishiwata
    • Yoko Matsuda
    • Tetsushi Yamamoto
    • Hirobumi Asakura
    • Toshiyuki Takeshita
    • Zenya Naito
  • View Affiliations / Copyright

    Affiliations: Departments of Pathology and Integrative Oncological Pathology, Nippon Medical School, Tokyo 113-8602, Japan, Division of Reproductive Medicine, Perinatology and Gynecologic Oncology, Graduate School of Medicine, Nippon Medical School, Tokyo 113-8603, Japan
    Copyright: © Sato et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 441-448
    |
    Published online on: May 10, 2012
       https://doi.org/10.3892/ijo.2012.1473
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Abstract

Nestin expression reportedly correlates with aggressive growth, metastasis, poor prognosis and presence of cancer stem cells (CSCs) in various tumors. In this study, we determined the expression and role of nestin in cervical intraepithelial neoplasia (CIN) and cervical cancer. We performed immunohistochemical and in situ hybridization analyses of nestin in 26 cases for each stage of CIN and 55 cervical cancer tissue samples. To examine the role of nestin in cervical cancer cells, we stably transfected expression vectors containing nestin cDNA into ME-180 cells. We studied the effects of increased nestin expression on cell proliferation, cell motility, invasion as well as sphere and soft agar formation. Nestin was not localized in the squamous epithelium in normal cervical tissues, but it was weakly expressed in the basal squamous epithelium of CIN 1. In CIN 2, nestin was localized to the basal to lower 2/3 of the squamous epithelium, whereas in CIN 3, it was localized to the majority of the squamous epithelium. Nestin was detected in all cases of invasive cervical cancer. Nestin mRNA was expressed in both ME-180 and CaSki cells. Growth rate, cell motility and invasion ability of stably nestin-transfected ME-180 cells were not different from empty vector-transfected ME-180 (mock cells). However, the nestin-transfected ME-180 cells formed more colonies and spheres compared to the mock cells. These findings suggest that nestin plays important roles in carcinogenesis and tumor formation of cervical cancer cells. Nestin may closely correlate with regulation of CSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Sato A, Ishiwata T, Matsuda Y, Yamamoto T, Asakura H, Takeshita T and Naito Z: Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer. Int J Oncol 41: 441-448, 2012.
APA
Sato, A., Ishiwata, T., Matsuda, Y., Yamamoto, T., Asakura, H., Takeshita, T., & Naito, Z. (2012). Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer. International Journal of Oncology, 41, 441-448. https://doi.org/10.3892/ijo.2012.1473
MLA
Sato, A., Ishiwata, T., Matsuda, Y., Yamamoto, T., Asakura, H., Takeshita, T., Naito, Z."Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer". International Journal of Oncology 41.2 (2012): 441-448.
Chicago
Sato, A., Ishiwata, T., Matsuda, Y., Yamamoto, T., Asakura, H., Takeshita, T., Naito, Z."Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer". International Journal of Oncology 41, no. 2 (2012): 441-448. https://doi.org/10.3892/ijo.2012.1473
Copy and paste a formatted citation
x
Spandidos Publications style
Sato A, Ishiwata T, Matsuda Y, Yamamoto T, Asakura H, Takeshita T and Naito Z: Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer. Int J Oncol 41: 441-448, 2012.
APA
Sato, A., Ishiwata, T., Matsuda, Y., Yamamoto, T., Asakura, H., Takeshita, T., & Naito, Z. (2012). Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer. International Journal of Oncology, 41, 441-448. https://doi.org/10.3892/ijo.2012.1473
MLA
Sato, A., Ishiwata, T., Matsuda, Y., Yamamoto, T., Asakura, H., Takeshita, T., Naito, Z."Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer". International Journal of Oncology 41.2 (2012): 441-448.
Chicago
Sato, A., Ishiwata, T., Matsuda, Y., Yamamoto, T., Asakura, H., Takeshita, T., Naito, Z."Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer". International Journal of Oncology 41, no. 2 (2012): 441-448. https://doi.org/10.3892/ijo.2012.1473
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