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Article

Genome-wide screening for methylation-silenced genes in colorectal cancer

  • Authors:
    • Ahmed Khamas
    • Toshiaki Ishikawa
    • Kaoru Mogushi
    • Satoru Iida
    • Megumi Ishiguro
    • Hiroshi Tanaka
    • Hiroyuki Uetake
    • Kenichi Sugihara
  • View Affiliations / Copyright

    Affiliations: Department of Surgical Oncology, Graduate School of Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan, Department of Computational Biology, Graduate School of Biomedical Science, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan, Department of Translational Oncology, Graduate School of Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan
  • Pages: 490-496
    |
    Published online on: May 30, 2012
       https://doi.org/10.3892/ijo.2012.1500
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Abstract

Identification of methylation-silenced genes in colorectal cancer (CRC) is of great importance. We employed oligonucleotide microarrays to identify differences in global gene expression of five CRC cell lines (HCT116, RKO, Colo320, SW480 and HT29) that were analyzed before and after treatment with 5-aza-2'-deoxycitidine. Selected candidates were subjected to methylation-specific PCR and real-time quantitative reverse transcription-PCR using 15 CRC cell lines and 23 paired tumor and normal samples from CRC patients. After 5-aza-2'-deoxycitidine treatment, 139 genes were re-expressed in all 5 CRC cell lines collectively with a fold change of more than 1.5 in at least one cell line. These genes include known methylated and silenced genes in CRC. After applying study selection criteria we identified 20 candidates. The GADD45B and THSD1 genes were selected for further analysis. Among 15 colon cancer cell lines, methylation was only identified in THSD1 (27%). THSD1 methy­lation was subsequently investigated in 23 colorectal tumors and methylation was detected in 9% of the analyzed samples; the observed promoter hypermethylation was cancer-specific. THSD1 mRNA down-regulation was observed in tumor tissues. This genome-wide screening led to the identification of genes putatively affected by methylation in CRC. The THSD1 gene may play a role in the tumorigenesis of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Khamas A, Ishikawa T, Mogushi K, Iida S, Ishiguro M, Tanaka H, Uetake H and Sugihara K: Genome-wide screening for methylation-silenced genes in colorectal cancer. Int J Oncol 41: 490-496, 2012.
APA
Khamas, A., Ishikawa, T., Mogushi, K., Iida, S., Ishiguro, M., Tanaka, H. ... Sugihara, K. (2012). Genome-wide screening for methylation-silenced genes in colorectal cancer. International Journal of Oncology, 41, 490-496. https://doi.org/10.3892/ijo.2012.1500
MLA
Khamas, A., Ishikawa, T., Mogushi, K., Iida, S., Ishiguro, M., Tanaka, H., Uetake, H., Sugihara, K."Genome-wide screening for methylation-silenced genes in colorectal cancer". International Journal of Oncology 41.2 (2012): 490-496.
Chicago
Khamas, A., Ishikawa, T., Mogushi, K., Iida, S., Ishiguro, M., Tanaka, H., Uetake, H., Sugihara, K."Genome-wide screening for methylation-silenced genes in colorectal cancer". International Journal of Oncology 41, no. 2 (2012): 490-496. https://doi.org/10.3892/ijo.2012.1500
Copy and paste a formatted citation
x
Spandidos Publications style
Khamas A, Ishikawa T, Mogushi K, Iida S, Ishiguro M, Tanaka H, Uetake H and Sugihara K: Genome-wide screening for methylation-silenced genes in colorectal cancer. Int J Oncol 41: 490-496, 2012.
APA
Khamas, A., Ishikawa, T., Mogushi, K., Iida, S., Ishiguro, M., Tanaka, H. ... Sugihara, K. (2012). Genome-wide screening for methylation-silenced genes in colorectal cancer. International Journal of Oncology, 41, 490-496. https://doi.org/10.3892/ijo.2012.1500
MLA
Khamas, A., Ishikawa, T., Mogushi, K., Iida, S., Ishiguro, M., Tanaka, H., Uetake, H., Sugihara, K."Genome-wide screening for methylation-silenced genes in colorectal cancer". International Journal of Oncology 41.2 (2012): 490-496.
Chicago
Khamas, A., Ishikawa, T., Mogushi, K., Iida, S., Ishiguro, M., Tanaka, H., Uetake, H., Sugihara, K."Genome-wide screening for methylation-silenced genes in colorectal cancer". International Journal of Oncology 41, no. 2 (2012): 490-496. https://doi.org/10.3892/ijo.2012.1500
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