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September 2012 Volume 41 Issue 3

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Article

A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation

  • Authors:
    • Kang-Mi Kim
    • Myung-Ha Song
    • Min-Ju Kim
    • Sayeema Daudi
    • Anthony Miliotto
    • Lloyd Old
    • Kunle Odunsi
    • Sang-Yull Lee
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology and Immunology, School of Medicine, Pusan National University, Yangsan-si, Gyeongsangnam-do 626-770, Republic of Korea, Department of Biochemistry, School of Medicine, Pusan National University, Yangsan-si, Gyeongsangnam-do 626-770, Republic of Korea, Department of Gynecologic Oncology and Center for Immunotherapy Roswell Park Cancer Institute, New York, NY 142634, USA, Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA
  • Pages: 1139-1147
    |
    Published online on: June 6, 2012
       https://doi.org/10.3892/ijo.2012.1508
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Abstract

SEREX has proven to be a powerful method that takes advantage of the presence of spontaneous humoral immune response in some cancer patients. In this study, immunoscreening of normal testis and two ovarian cancer cell line cDNA expression libraries with sera from ovarian cancer patients led to the isolation of 75 independent antigens, designated KP-OVA-1 through KP-OVA-75. Of these, RT-PCR showed KP-OVA-52 to be expressed strongly in normal testis, in ovarian cancer cell lines (3/9) and in ovarian cancer tissues (1/17). The expression of KP-OVA-52 in cancer cells is also induced by the demethylating agent 5‑aza‑2'‑deoxycytidine (ADC). To test immunogenicity, we used the Serum Antibody Detection Assay (SADA) to analyze anti-IgG antibodies against the 75 antigens that were initially isolated by SEREX. Four of the 75 antigens (KP‑OVA‑25, KP‑OVA‑35, KP‑OVA‑68 and KP‑OVA‑73) reacted exclusively with sera from cancer patients. However, KP‑OVA‑52 reacted with 1 of 20 ovarian cancer sera. These data suggest that the KP-OVA-52 can be considered a novel CT antigen that is regulated by DNA methylation.
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Copy and paste a formatted citation
Spandidos Publications style
Kim K, Song M, Kim M, Daudi S, Miliotto A, Old L, Odunsi K and Lee S: A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation. Int J Oncol 41: 1139-1147, 2012.
APA
Kim, K., Song, M., Kim, M., Daudi, S., Miliotto, A., Old, L. ... Lee, S. (2012). A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation. International Journal of Oncology, 41, 1139-1147. https://doi.org/10.3892/ijo.2012.1508
MLA
Kim, K., Song, M., Kim, M., Daudi, S., Miliotto, A., Old, L., Odunsi, K., Lee, S."A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation". International Journal of Oncology 41.3 (2012): 1139-1147.
Chicago
Kim, K., Song, M., Kim, M., Daudi, S., Miliotto, A., Old, L., Odunsi, K., Lee, S."A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation". International Journal of Oncology 41, no. 3 (2012): 1139-1147. https://doi.org/10.3892/ijo.2012.1508
Copy and paste a formatted citation
x
Spandidos Publications style
Kim K, Song M, Kim M, Daudi S, Miliotto A, Old L, Odunsi K and Lee S: A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation. Int J Oncol 41: 1139-1147, 2012.
APA
Kim, K., Song, M., Kim, M., Daudi, S., Miliotto, A., Old, L. ... Lee, S. (2012). A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation. International Journal of Oncology, 41, 1139-1147. https://doi.org/10.3892/ijo.2012.1508
MLA
Kim, K., Song, M., Kim, M., Daudi, S., Miliotto, A., Old, L., Odunsi, K., Lee, S."A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation". International Journal of Oncology 41.3 (2012): 1139-1147.
Chicago
Kim, K., Song, M., Kim, M., Daudi, S., Miliotto, A., Old, L., Odunsi, K., Lee, S."A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation". International Journal of Oncology 41, no. 3 (2012): 1139-1147. https://doi.org/10.3892/ijo.2012.1508
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