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Article

Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma

  • Authors:
    • Qing Shao
    • Pengfei Ren
    • Yang Li
    • Bo Peng
    • Liping Dai
    • Ningjing  Lei
    • Wu Yao
    • Gang Zhao
    • Linggen Li
    • Jianying Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China
  • Pages: 1061-1067
    |
    Published online on: June 12, 2012
       https://doi.org/10.3892/ijo.2012.1515
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Abstract

Hepatocellular carcinoma (HCC) is a type of cancer with a very poor prognosis. Although α-fetoprotein (AFP) is the most effective marker available to detect HCC, the sensitivity and specificity are not optimal. Therefore, there is a need for the development of more sensitive and specific methods that can supplement AFP in the early detection of this cancer. In this study, autoantibody responses to glucose-regulated protein 78 (GRP78) were evaluated by enzyme-linked immunosorbent assay (ELISA), western blotting and indirect immunofluorescence assay in sera from patients with HCC, liver cirrhosis (LC) and chronic hepatitis (CH), as well as from normal human individuals. Immunohistochemistry (IHC) with tissue array slides was also preformed to analyze protein expression profiles of GRP78 in HCC and control tissues. The prevalence of autoantibodies against GRP78 was 35.5% (27/76) in HCC, which was significantly higher than that in LC, CH and normal human sera (NHS; P<0.01). The average titer of autoantibodies against GRP78 in HCC sera was higher compared to that in LC, CH and NHS(P<0.01). When both autoantibodies against GRP78 and AFP were used simultaneously as diagnostic markers, sensitivity reached 71.4%. Our data indicate that anti-GRP78 autoantibodies may be potential diagnostic markers for HCC, especially in conjunction with AFP.
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Copy and paste a formatted citation
Spandidos Publications style
Shao Q, Ren P, Li Y, Peng B, Dai L, Lei N, Yao W, Zhao G, Li L, Zhang J, Zhang J, et al: Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma. Int J Oncol 41: 1061-1067, 2012.
APA
Shao, Q., Ren, P., Li, Y., Peng, B., Dai, L., Lei, N. ... Zhang, J. (2012). Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma. International Journal of Oncology, 41, 1061-1067. https://doi.org/10.3892/ijo.2012.1515
MLA
Shao, Q., Ren, P., Li, Y., Peng, B., Dai, L., Lei, N., Yao, W., Zhao, G., Li, L., Zhang, J."Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma". International Journal of Oncology 41.3 (2012): 1061-1067.
Chicago
Shao, Q., Ren, P., Li, Y., Peng, B., Dai, L., Lei, N., Yao, W., Zhao, G., Li, L., Zhang, J."Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma". International Journal of Oncology 41, no. 3 (2012): 1061-1067. https://doi.org/10.3892/ijo.2012.1515
Copy and paste a formatted citation
x
Spandidos Publications style
Shao Q, Ren P, Li Y, Peng B, Dai L, Lei N, Yao W, Zhao G, Li L, Zhang J, Zhang J, et al: Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma. Int J Oncol 41: 1061-1067, 2012.
APA
Shao, Q., Ren, P., Li, Y., Peng, B., Dai, L., Lei, N. ... Zhang, J. (2012). Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma. International Journal of Oncology, 41, 1061-1067. https://doi.org/10.3892/ijo.2012.1515
MLA
Shao, Q., Ren, P., Li, Y., Peng, B., Dai, L., Lei, N., Yao, W., Zhao, G., Li, L., Zhang, J."Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma". International Journal of Oncology 41.3 (2012): 1061-1067.
Chicago
Shao, Q., Ren, P., Li, Y., Peng, B., Dai, L., Lei, N., Yao, W., Zhao, G., Li, L., Zhang, J."Autoantibodies against glucose-regulated protein 78 as serological diagnostic biomarkers in hepatocellular carcinoma". International Journal of Oncology 41, no. 3 (2012): 1061-1067. https://doi.org/10.3892/ijo.2012.1515
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