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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 2012 Volume 41 Issue 3

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Silibinin, a natural flavonoid, modulates the early expression of chemoprevention biomarkers in a preclinical model of colon carcinogenesis

  • Authors:
    • Henriette Kauntz
    • Souad Bousserouel
    • Francine Gosse
    • Jacques Marescaux
    • Francis Raul
  • View Affiliations / Copyright

    Affiliations: Laboratory of Nutritional Cancer Prevention, EA 4438, Faculty of Medicine, IRCAD, University of Strasbourg, Strasbourg, France, IRCAD - EITS, Strasbourg, France
    Copyright: © Kauntz et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 849-854
    |
    Published online on: June 25, 2012
       https://doi.org/10.3892/ijo.2012.1526
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Abstract

The flavonolignan silibinin, the major biologically active compound of the milk thistle (Silybum marianum), has been shown to possess anticancer properties in a variety of epithelial cancers. The present study investigated the potential of silibinin as a chemopreventive agent in colon carcinogenesis. The rat azoxymethane (AOM)-induced colon carcinogenesis model was used because of its molecular and clinical similarities to sporadic human colorectal cancer. One week after AOM injection (post-initiation), Wistar rats received daily intragastric feeding of 300 mg silibinin/kg body weight per day until their sacrifice after 7 weeks of treatment. Silibinin-treated rats exhibited a 2-fold reduction in the number of AOM-induced hyperproliferative crypts and aberrant crypt foci in the colon compared to AOM-injected control rats receiving the vehicle. Silibinin-induced apoptosis in the colon mucosal cells was demonstrated by flow cytometry after propodium iodide staining and by colorimetric measurement of caspase-3 activity. Mechanisms involved in silibinin-induced apoptosis included the downregulation of the anti-apoptotic protein Bcl-2 and upregulation of the pro-apoptotic protein Bax, inverting the Bcl-2/Bax ratio to <1. This modulation already takes place at the mRNA expression level as shown by real-time RT-PCR. Furthermore, silibinin treatment significantly (P<0.01) decreased the genetic expression of biomarkers of the inflammatory response such as IL1β, TNFα and their downstream target MMP7, all of them shown to be upregulated during colon carcinogenesis. The downregulation of MMP7 protein was confirmed by western blot analysis. The present findings show the ability of silibinin to shift the disturbed balance between cell renewal and cell death in colon carcinogenesis in rats previously injected with the carcinogen AOM. Silibinin administered via intragastric feeding exhibited potent pro-apoptotic, anti-inflammatory and multi-targeted effects at the molecular level. The effective reduction of preneoplastic lesions by silibinin supports its use as a natural agent for colon cancer chemoprevention.
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Copy and paste a formatted citation
Spandidos Publications style
Kauntz H, Bousserouel S, Gosse F, Marescaux J and Raul F: Silibinin, a natural flavonoid, modulates the early expression of chemoprevention biomarkers in a preclinical model of colon carcinogenesis. Int J Oncol 41: 849-854, 2012.
APA
Kauntz, H., Bousserouel, S., Gosse, F., Marescaux, J., & Raul, F. (2012). Silibinin, a natural flavonoid, modulates the early expression of chemoprevention biomarkers in a preclinical model of colon carcinogenesis. International Journal of Oncology, 41, 849-854. https://doi.org/10.3892/ijo.2012.1526
MLA
Kauntz, H., Bousserouel, S., Gosse, F., Marescaux, J., Raul, F."Silibinin, a natural flavonoid, modulates the early expression of chemoprevention biomarkers in a preclinical model of colon carcinogenesis". International Journal of Oncology 41.3 (2012): 849-854.
Chicago
Kauntz, H., Bousserouel, S., Gosse, F., Marescaux, J., Raul, F."Silibinin, a natural flavonoid, modulates the early expression of chemoprevention biomarkers in a preclinical model of colon carcinogenesis". International Journal of Oncology 41, no. 3 (2012): 849-854. https://doi.org/10.3892/ijo.2012.1526
Copy and paste a formatted citation
x
Spandidos Publications style
Kauntz H, Bousserouel S, Gosse F, Marescaux J and Raul F: Silibinin, a natural flavonoid, modulates the early expression of chemoprevention biomarkers in a preclinical model of colon carcinogenesis. Int J Oncol 41: 849-854, 2012.
APA
Kauntz, H., Bousserouel, S., Gosse, F., Marescaux, J., & Raul, F. (2012). Silibinin, a natural flavonoid, modulates the early expression of chemoprevention biomarkers in a preclinical model of colon carcinogenesis. International Journal of Oncology, 41, 849-854. https://doi.org/10.3892/ijo.2012.1526
MLA
Kauntz, H., Bousserouel, S., Gosse, F., Marescaux, J., Raul, F."Silibinin, a natural flavonoid, modulates the early expression of chemoprevention biomarkers in a preclinical model of colon carcinogenesis". International Journal of Oncology 41.3 (2012): 849-854.
Chicago
Kauntz, H., Bousserouel, S., Gosse, F., Marescaux, J., Raul, F."Silibinin, a natural flavonoid, modulates the early expression of chemoprevention biomarkers in a preclinical model of colon carcinogenesis". International Journal of Oncology 41, no. 3 (2012): 849-854. https://doi.org/10.3892/ijo.2012.1526
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