Open Access

Simultaneous siRNA-mediated knockdown of antiapoptotic BCL2, Bcl-xL, XIAP and survivin in bladder cancer cells

  • Authors:
    • Doreen Kunze
    • Kai Kraemer
    • Kati Erdmann
    • Michael Froehner
    • Manfred  P. Wirth
    • Susanne Fuessel
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  • Published online on: July 6, 2012     https://doi.org/10.3892/ijo.2012.1549
  • Pages: 1271-1277
  • Copyright: © Kunze et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Bladder cancer (BCa) represents the ninth most common malignancy worldwide. Despite intensive treatment with surgery and chemotherapy the prognosis for BCa patients particularly at advanced stages is poor. The ability to evade apoptosis is a hallmark of cancer cells. Since the antiapoptotic genes BCL2, Bcl-xL, XIAP and survivin are frequently upregulated in BCa tissues, their combined siRNA-mediated knockdown might be more potent in decreasing BCa growth than the single inhibition of one target. Against each target two siRNAs were selected that specifically reduced the mRNA and protein levels of their appropriate target in EJ28 and J82 BCa cells. Inhibition of survivin provoked the strongest antiproliferative effect of all single target treatments, for example cell counts decreased by 50%. Simultaneous targeting of all four antiapoptotic genes downregulated expression levels of all targets and mediated significant reductions in cell viability and cell counts as well as induction of apoptosis. In EJ28 cells, combined knockdown of BCL2, Bcl-xL, XIAP and survivin caused a 2.5-fold enhancement in apoptosis rate and reduced cellular viability by 40%. Therefore, simultaneous knockdown of antiapoptotic BCL2, Bcl‑xL, XIAP and survivin may represent a promising treatment option for bladder cancer.
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October 2012
Volume 41 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kunze D, Kraemer K, Erdmann K, Froehner M, Wirth MP and Fuessel S: Simultaneous siRNA-mediated knockdown of antiapoptotic BCL2, Bcl-xL, XIAP and survivin in bladder cancer cells. Int J Oncol 41: 1271-1277, 2012
APA
Kunze, D., Kraemer, K., Erdmann, K., Froehner, M., Wirth, M.P., & Fuessel, S. (2012). Simultaneous siRNA-mediated knockdown of antiapoptotic BCL2, Bcl-xL, XIAP and survivin in bladder cancer cells. International Journal of Oncology, 41, 1271-1277. https://doi.org/10.3892/ijo.2012.1549
MLA
Kunze, D., Kraemer, K., Erdmann, K., Froehner, M., Wirth, M. P., Fuessel, S."Simultaneous siRNA-mediated knockdown of antiapoptotic BCL2, Bcl-xL, XIAP and survivin in bladder cancer cells". International Journal of Oncology 41.4 (2012): 1271-1277.
Chicago
Kunze, D., Kraemer, K., Erdmann, K., Froehner, M., Wirth, M. P., Fuessel, S."Simultaneous siRNA-mediated knockdown of antiapoptotic BCL2, Bcl-xL, XIAP and survivin in bladder cancer cells". International Journal of Oncology 41, no. 4 (2012): 1271-1277. https://doi.org/10.3892/ijo.2012.1549