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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 2012 Volume 41 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 2012 Volume 41 Issue 4

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Article

Biological influence of brain-derived neurotrophic factor on breast cancer cells

  • Authors:
    • Xiaomei Yang
    • Tracey A. Martin
    • Wen G. Jiang
  • View Affiliations / Copyright

    Affiliations: Metastasis and Angiogenesis Research Group, Institute of Cancer and Genetics, Cardiff University School of Medicine, Cardiff, CF14 4XN, UK
  • Pages: 1541-1546
    |
    Published online on: August 6, 2012
       https://doi.org/10.3892/ijo.2012.1581
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Abstract

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin superfamily which has been indicated in the pathophysiology of the nervous system and is important in a number of neurological and psychological conditions. Recently, BDNF was also shown to play a role in the development and progression of solid tumour myeloma. It has been reported that BDNF is aberrantly expressed in human breast cancer and that a raised level of BDNF is associated with poor clinical outcome and reduced survival. The present study investigated the role of BDNF in human breast cancer. A panel of human breast cancer cells was used and the expression profile of BDNF was evaluated using RT-PCR. We constructed a set of anti-BDNF transgenes which were used to transfect breast cancer cells in order to generate BDNF knocked down cells. The impact of BDNF knockdown on growth and apoptosis was evaluated. Statistical analysis was performed using SPSS. P<0.05 was considered statistically significant. BDNF gene transcripts were successfully detected in the breast cancer cell lines MCF-7, MDA-MB-231 and ZR75-1 MDA-MB-231 and MCF-7 wild-type cells were subject to transfection of anti-BDNF transgenes, followed by the establishment of BDNF knocked down sublines. Knockdown of BDNF in MDA-MB-231 and MCF-7 cell lines resulted in decreased rates of growth and proliferation. Analysis of apoptosis showed that cell apoptosis was increased in cells stably transfected with ribozymes for BDNF compared with the vector control. It is concluded that BDNF, a neurotrophic growth factor aberrantly expressed in cancers such as breast cancer, has a profound impact on the cellular behaviour of breast cancer cells and that BDNF is associated with a reduction of the apoptosis of breast cancer. BDNF is, therefore, a potential therapeutic target in breast cancer and its effect in human breast cancer requires further investigation.
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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Martin TA and Jiang WG: Biological influence of brain-derived neurotrophic factor on breast cancer cells. Int J Oncol 41: 1541-1546, 2012.
APA
Yang, X., Martin, T.A., & Jiang, W.G. (2012). Biological influence of brain-derived neurotrophic factor on breast cancer cells. International Journal of Oncology, 41, 1541-1546. https://doi.org/10.3892/ijo.2012.1581
MLA
Yang, X., Martin, T. A., Jiang, W. G."Biological influence of brain-derived neurotrophic factor on breast cancer cells". International Journal of Oncology 41.4 (2012): 1541-1546.
Chicago
Yang, X., Martin, T. A., Jiang, W. G."Biological influence of brain-derived neurotrophic factor on breast cancer cells". International Journal of Oncology 41, no. 4 (2012): 1541-1546. https://doi.org/10.3892/ijo.2012.1581
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Martin TA and Jiang WG: Biological influence of brain-derived neurotrophic factor on breast cancer cells. Int J Oncol 41: 1541-1546, 2012.
APA
Yang, X., Martin, T.A., & Jiang, W.G. (2012). Biological influence of brain-derived neurotrophic factor on breast cancer cells. International Journal of Oncology, 41, 1541-1546. https://doi.org/10.3892/ijo.2012.1581
MLA
Yang, X., Martin, T. A., Jiang, W. G."Biological influence of brain-derived neurotrophic factor on breast cancer cells". International Journal of Oncology 41.4 (2012): 1541-1546.
Chicago
Yang, X., Martin, T. A., Jiang, W. G."Biological influence of brain-derived neurotrophic factor on breast cancer cells". International Journal of Oncology 41, no. 4 (2012): 1541-1546. https://doi.org/10.3892/ijo.2012.1581
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