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Article

Honokiol exerts an anticancer effect in T98G human glioblastoma cells through the induction of apoptosis and the regulation of adhesion molecules

  • Authors:
    • Jae Ju Jeong
    • Jae Heun Lee
    • Ki Churl Chang
    • Hye Jung Kim
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, School of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea
  • Pages: 1358-1364
    |
    Published online on: August 7, 2012
       https://doi.org/10.3892/ijo.2012.1582
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Abstract

Glioblastoma is one of the most lethal and common malignant human brain tumors, with aggressive proliferation and highly invasive properties. Honokiol derived from Magnolia officinalis is able to cross the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCSFB), suggesting a strong possibility that it could be an effective drug for the treatment of brain tumors, including glioblastoma. Thus, we investigated the effects of honokiol on the expression of adhesion molecules in TNF-α-stimulated endothelial cells, and cancer growth and invasion were determined in T98G human glioblastoma cells. Honokiol dose-dependently inhibited the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) stimulated with TNF-α for 6 h. Pretreatment with honokiol significantly reduced the adhesion of T98G cells to HUVECs. Moreover, honokiol inhibited the invasion of T98G cells, suggesting that honokiol has an anti-metastatic effect. In addition, honokiol increased the cytotoxicity of T98G cells in a dose- and time-dependent manner as assayed by MTT. TUNEL assay showed that honokiol significantly induced apoptosis in T98G cells at doses of 10 µM or more. The induction of apoptotic cell death was mediated by the downregulation of the anti-apoptotic protein Bcl-2 and the upregulation of the pro-apoptotic protein Bax. Taken together, the results of this study suggest that honokiol exerts an anticancer effect by preventing metastasis and inducing apoptotic cell death of brain tumor cells.
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Copy and paste a formatted citation
Spandidos Publications style
Jeong JJ, Lee JH, Chang KC and Kim H : Honokiol exerts an anticancer effect in T98G human glioblastoma cells through the induction of apoptosis and the regulation of adhesion molecules. Int J Oncol 41: 1358-1364, 2012.
APA
Jeong, J.J., Lee, J.H., Chang, K.C., & Kim, H. . (2012). Honokiol exerts an anticancer effect in T98G human glioblastoma cells through the induction of apoptosis and the regulation of adhesion molecules. International Journal of Oncology, 41, 1358-1364. https://doi.org/10.3892/ijo.2012.1582
MLA
Jeong, J. J., Lee, J. H., Chang, K. C., Kim, H. ."Honokiol exerts an anticancer effect in T98G human glioblastoma cells through the induction of apoptosis and the regulation of adhesion molecules". International Journal of Oncology 41.4 (2012): 1358-1364.
Chicago
Jeong, J. J., Lee, J. H., Chang, K. C., Kim, H. ."Honokiol exerts an anticancer effect in T98G human glioblastoma cells through the induction of apoptosis and the regulation of adhesion molecules". International Journal of Oncology 41, no. 4 (2012): 1358-1364. https://doi.org/10.3892/ijo.2012.1582
Copy and paste a formatted citation
x
Spandidos Publications style
Jeong JJ, Lee JH, Chang KC and Kim H : Honokiol exerts an anticancer effect in T98G human glioblastoma cells through the induction of apoptosis and the regulation of adhesion molecules. Int J Oncol 41: 1358-1364, 2012.
APA
Jeong, J.J., Lee, J.H., Chang, K.C., & Kim, H. . (2012). Honokiol exerts an anticancer effect in T98G human glioblastoma cells through the induction of apoptosis and the regulation of adhesion molecules. International Journal of Oncology, 41, 1358-1364. https://doi.org/10.3892/ijo.2012.1582
MLA
Jeong, J. J., Lee, J. H., Chang, K. C., Kim, H. ."Honokiol exerts an anticancer effect in T98G human glioblastoma cells through the induction of apoptosis and the regulation of adhesion molecules". International Journal of Oncology 41.4 (2012): 1358-1364.
Chicago
Jeong, J. J., Lee, J. H., Chang, K. C., Kim, H. ."Honokiol exerts an anticancer effect in T98G human glioblastoma cells through the induction of apoptosis and the regulation of adhesion molecules". International Journal of Oncology 41, no. 4 (2012): 1358-1364. https://doi.org/10.3892/ijo.2012.1582
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