The mTOR inhibitor RAD001 augments radiation-induced growth inhibition in a hepatocellular carcinoma cell line by increasing autophagy

  • Authors:
    • Anaïs Altmeyer
    • Elodie Josset
    • Jean-Marc Denis
    • John  Gueulette
    • Jakobus  Slabbert
    • Didier Mutter
    • Georges Noël
    • Pierre  Bischoff
  • View Affiliations

  • Published online on: August 7, 2012     https://doi.org/10.3892/ijo.2012.1583
  • Pages: 1381-1386
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Abstract

Treatment of hepatocellular carcinoma (HCC) is a major concern for physicians as its response to chemotherapy and radiotherapy remains generally poor, due, in part, to intrinsic resistance to either form of treatment. We previously reported that an irradiation with fast neutrons, which are high-linear energy transfer (LET) particles, massively induced autophagic cell death in the human HCC SK-Hep1 cell line. In the present study, we tested the capacity of the mammalian target of rapamycin (mTOR) inhibitor RAD001 to augment the cytotoxicity of low and high-LET radiation in these cells. As mTOR is a key component in a series of pathways involved in tumor growth and development, it represents a potential molecular target for cancer treatment. Results indicate that RAD001, at clinically relevant nanomolar concentrations, enhances the efficacy of both high- and low-LET radiation in SK-Hep1 cells, and that the induction of autophagy may account for this effect. However, fast neutrons were found to be more efficient at reducing tumor cell growth than low-LET radiation.
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October 2012
Volume 41 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Altmeyer A, Josset E, Denis J, Gueulette J, Slabbert J, Mutter D, Noël G and Bischoff P: The mTOR inhibitor RAD001 augments radiation-induced growth inhibition in a hepatocellular carcinoma cell line by increasing autophagy. Int J Oncol 41: 1381-1386, 2012.
APA
Altmeyer, A., Josset, E., Denis, J., Gueulette, J., Slabbert, J., Mutter, D. ... Bischoff, P. (2012). The mTOR inhibitor RAD001 augments radiation-induced growth inhibition in a hepatocellular carcinoma cell line by increasing autophagy. International Journal of Oncology, 41, 1381-1386. https://doi.org/10.3892/ijo.2012.1583
MLA
Altmeyer, A., Josset, E., Denis, J., Gueulette, J., Slabbert, J., Mutter, D., Noël, G., Bischoff, P."The mTOR inhibitor RAD001 augments radiation-induced growth inhibition in a hepatocellular carcinoma cell line by increasing autophagy". International Journal of Oncology 41.4 (2012): 1381-1386.
Chicago
Altmeyer, A., Josset, E., Denis, J., Gueulette, J., Slabbert, J., Mutter, D., Noël, G., Bischoff, P."The mTOR inhibitor RAD001 augments radiation-induced growth inhibition in a hepatocellular carcinoma cell line by increasing autophagy". International Journal of Oncology 41, no. 4 (2012): 1381-1386. https://doi.org/10.3892/ijo.2012.1583