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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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November 2012 Volume 41 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells

  • Authors:
    • Tetsuji Tanaka
    • Saori Toujima
    • Junko Tanaka
  • View Affiliations / Copyright

    Affiliations: Santamaria Hospital, Ibaraki, Osaka 567-0884, Japan, Department of Obstetrics and Gynecology, Wakayama Medical University, Wakayama 641-0012, Japan, Graduate School of Intercultural Studies, Kobe University, Nada-ku, Kobe 657-8501, Japan
  • Pages: 1837-1844
    |
    Published online on: August 22, 2012
       https://doi.org/10.3892/ijo.2012.1600
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Abstract

To investigate acquired paclitaxel (PTX) resistance in cancer cells, we established five monoclonal PTX-resistant cell lines from HEC-1 human endometrial adenocarcinoma cells by means of long-term PTX-exposed cultures and limiting dilution cultures. The established PTX-resistant subclones showed apparent resistance to PTX-induced DNA fragmentation but not to PTX-induced growth suppression. None of the five PTX-resistant subclones showed apparent resistance to other anticancer drugs such as cisplatin, etoposide, 5-fluorouracil, pirarubicin-HCl, 4-hydroxy-cyclophosphamide or mitomycin C. Semiquantitative flow cytometric analysis revealed no apparent differential expression of 17 molecules that were previously reported to regulate apoptosis or drug resistance, between the five PTX-resistant subclones and the parent cells. Karyotyping analysis revealed common changes in chromosomes 4 and 18 in the five PTX-resistant subclones but not in the HEC-1 parent cells. These results indicate that PTX-induced growth suppression is regulated by different mechanisms from those involved in PTX-induced apoptosis. It was concluded that these established PTX-resistant subclones can be useful models in studies related to the prevention or treatment of recurrent cancers after PTX chemotherapy.
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1. 

Chen YX, Wang Y, Fu CC, Diao F, Song LN, Li ZB, Yang R and Lu J: Dexamethasone enhances cell resistance to chemotherapy by increasing adhesion to extracellular matrix in human ovarian cancer cells. Endocr Relat Cancer. 17:39–50. 2010. View Article : Google Scholar : PubMed/NCBI

2. 

Dong Y, Tan OL, Loessner D, Stephens C, Walpole C, Boyle GM, Parsons PG and Clements JA: Kallikrein-related peptidase 7 promotes multicellular aggregation via the alpha(5) beta(1) integrin pathway and paclitaxel chemoresistance in serous epithelial ovarian carcinoma. Cancer Res. 70:2624–2633. 2010. View Article : Google Scholar

3. 

Loessner D, Stok KS, Lutolf MP, Hutmacher DW, Clements JA and Rizzi SC: Bioengineered 3D platform to explore cell-ECM interactions and drug resistance of epithelial ovarian cancer cells. Biomaterials. 31:8494–8506. 2010. View Article : Google Scholar : PubMed/NCBI

4. 

Stumm S, Meyer A, Lindner M, Bastert G, Wallwiener D and Gückel B: Paclitaxel treatment of breast cancer cell lines modulates Fas/Fas ligand expression and induces apoptosis which can be inhibited through the CD40 receptor. Oncology. 66:101–111. 2004. View Article : Google Scholar

5. 

Huang Y, Ibrado AM, Reed JC, Bullock G, Ray S, Tang C and Bhalla K: Co-expression of several molecular mechanisms of multidrug resistance and their significance for paclitaxel cytotoxicity in human AML HL-60 cells. Leukemia. 11:253–257. 1997. View Article : Google Scholar : PubMed/NCBI

6. 

Kamazawa S, Kigawa J, Kanamori Y, Itamochi H, Sato S, Iba T and Terakawa N: Multidrug resistance gene-1 is a useful predictor of Paclitaxel-based chemotherapy for patients with ovarian cancer. Gynecol Oncol. 86:171–176. 2002. View Article : Google Scholar : PubMed/NCBI

7. 

Swanton C, Nicke B, Schuett M, Eklund AC, Ng C, Li Q, Hardcastle T, Lee A, Roy R, East P, Kschischo M, Endesfelder D, Wylie P, Kim SN, Chen JG, Howell M, Ried T, Habermann JK, Auer G, Brenton JD, Szallasi Z and Downward J: Chromosomal instability determines taxane response. Proc Natl Acad Sci USA. 106:8671–8676. 2009. View Article : Google Scholar : PubMed/NCBI

8. 

Satyaswaroop PG, Frost A and Gurpide E: Metabolism and effects of progesterone in the human endometrial adenocarcinoma cell line HEC-1. Steroids. 35:21–37. 1980. View Article : Google Scholar : PubMed/NCBI

9. 

Gibas Z, Prout GR, Conolly JG, Pontes JE and Sandberg AA: Nonrandom chromosome changes in transitional cell carcinoma of the bladder. Cancer Res. 44:1257–1264. 1984.PubMed/NCBI

10. 

Yoshida MA, Ohyashiki K, Ochi H, Gibas Z, Pontes JE, Prout GR Jr, Huben R and Sandberg AA: Cytogenetic studies of tumor tissue from patients with nonfamilial renal cell carcinoma. Cancer Res. 46:2139–2147. 1986.PubMed/NCBI

11. 

Yunis JJ: New chromosome techniques in the study of human neoplasia. Hum Pathol. 12:540–549. 1981. View Article : Google Scholar : PubMed/NCBI

12. 

Tanaka T, Bai T, Yukawa K, Utsunomiya T and Umesaki N: Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells. Oncol Rep. 14:941–948. 2005.

13. 

Tanaka T, Bai T and Toujima S: Establishment and characterization of monoclonal 5-fluorouracil-resistant cell lines derived from human endometrial adenocarcinoma. Int J Oncol. 37:731–736. 2010. View Article : Google Scholar : PubMed/NCBI

14. 

Tanaka T, Toujima S and Umesaki N: Growth-inhibitory signals by activin A do not affect anticancer drug-sensitivity and acquired multi-drig-resistance in human ovarian endometrioid adenocarcinoma OVK-18 cells. Oncol Rep. 11:667–671. 2004.

15. 

Tanaka T, Bai T, Yukawa K and Umesaki N: Optimal combination chemotherapy and chemoradiotherapy with etoposide for advanced cervical squamous cancer cells in vitro. Oncol Rep. 15:939–947. 2006.PubMed/NCBI

16. 

Ohbayashi M, Yasuda M, Kawakami I, Kohyama N, Kobayashi Y and Yamamoto T: Effect of interleukins response to ECM-induced acquisition of drug resistance in MCF-7 cells. Exp Oncol. 30:276–282. 2008.PubMed/NCBI

17. 

To K, Fotovati A, Reipas KM, Law JH, Hu K, Wang J, Astanehe A, Davies AH, Lee L, Stratford AL, Raouf A, Johnson P, Berquin IM, Royer HD, Eaves CJ and Dunn SE: Y-box binding protein-1 induces the expression of CD44 and CD49f leading to enhanced self-renewal, mammosphere growth, and drug resistance. Cancer Res. 70:2840–2851. 2010. View Article : Google Scholar : PubMed/NCBI

18. 

Işeri OD, Kars MD, Arpaci F and Gündüz U: Gene expression analysis of drug-resistant MCF-7 cells: implications for relation to extracellular matrix proteins. Cancer Chemother Pharmacol. 65:447–55. 2010.PubMed/NCBI

19. 

Lanni JS, Lowe SW, Licitra EJ, Liu JO and Jacks T: p53-independent apoptosis induced by paclitaxel through an indirect mechanism. Proc Natl Acad Sci USA. 94:9679–83. 1997. View Article : Google Scholar : PubMed/NCBI

20. 

Biswas RS, Cha HJ, Hardwick JM and Srivastava RK: Inhibition of drug-induced Fas ligand transcription and apoptosis by Bcl-XL. Mol Cell Biochem. 225:7–20. 2001. View Article : Google Scholar : PubMed/NCBI

21. 

Shivanna M and Srinivas SP: Microtubule stabilization opposes the (TNF-alpha)-induced loss in the barrier integrity of corneal endothelium. Exp Eye Res. 89:950–959. 2009. View Article : Google Scholar : PubMed/NCBI

22. 

Voorzanger-Rousselot N, Alberti L and Blay JY: CD40L induces multidrug resistance to apoptosis in breast carcinoma and lymphoma cells through caspase independent and dependent pathways. BMC Cancer. 18:752006. View Article : Google Scholar

23. 

Wang MY, Chen PS, Prakash E, Hsu HC, Huang HY, Lin MT, Chang KJ and Kuo ML: Connective tissue growth factor confers drug resistance in breast cancer through concomitant up-regulation of Bcl-xL and cIAP1. Cancer Res. 69:3482–3491. 2009. View Article : Google Scholar : PubMed/NCBI

24. 

Luo D, Cheng SC, Xie H and Xie Y: Effects of Bcl-2 and Bcl-XL protein levels on chemoresistance of hepatoblastoma HepG2 cell line. Biochem Cell Biol. 78:119–126. 2000. View Article : Google Scholar : PubMed/NCBI

25. 

Janssen K, Pohlmann S, Jänicke RU, Schulze-Osthoff K and Fischer U: Apaf-1 and caspase-9 deficiency prevents apoptosis in a Bax-controlled pathway and promotes clonogenic survival during paclitaxel treatment. Blood. 110:3662–3672. 2007. View Article : Google Scholar : PubMed/NCBI

26. 

Strobel T, Kraeft SK, Chen LB and Cannistra SA: BAX expression is associated with enhanced intracellular accumulation of paclitaxel: a novel role for BAX during chemotherapy-induced cell death. Cancer Res. 58:4776–4781. 1998.PubMed/NCBI

27. 

Kitazono M, Sumizawa T, Takebayashi Y, Chen ZS, Furukawa T, Nagayama S, Tani A, Takao S, Aikou T and Akiyama S: Multidrug resistance and the lung resistance-related protein in human colon carcinoma SW-620 cells. J Natl Cancer Inst. 91:1647–1653. 1999. View Article : Google Scholar : PubMed/NCBI

28. 

Tanaka T, Toujima S, Toyoda S, Takeuchi S and Umesaki N: Establishment and characterization of novel human uterine leiomyosarcoma cell lines. Int J Oncol. 35:125–131. 2010.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tanaka T, Toujima S and Tanaka J: Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells. Int J Oncol 41: 1837-1844, 2012.
APA
Tanaka, T., Toujima, S., & Tanaka, J. (2012). Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells. International Journal of Oncology, 41, 1837-1844. https://doi.org/10.3892/ijo.2012.1600
MLA
Tanaka, T., Toujima, S., Tanaka, J."Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells". International Journal of Oncology 41.5 (2012): 1837-1844.
Chicago
Tanaka, T., Toujima, S., Tanaka, J."Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells". International Journal of Oncology 41, no. 5 (2012): 1837-1844. https://doi.org/10.3892/ijo.2012.1600
Copy and paste a formatted citation
x
Spandidos Publications style
Tanaka T, Toujima S and Tanaka J: Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells. Int J Oncol 41: 1837-1844, 2012.
APA
Tanaka, T., Toujima, S., & Tanaka, J. (2012). Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells. International Journal of Oncology, 41, 1837-1844. https://doi.org/10.3892/ijo.2012.1600
MLA
Tanaka, T., Toujima, S., Tanaka, J."Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells". International Journal of Oncology 41.5 (2012): 1837-1844.
Chicago
Tanaka, T., Toujima, S., Tanaka, J."Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells". International Journal of Oncology 41, no. 5 (2012): 1837-1844. https://doi.org/10.3892/ijo.2012.1600
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