miR-203 inhibits the migration and invasion of esophageal squamous cell carcinoma by regulating LASP1

Takeshita,Nobuyoshi; Mori,Mikito; Kano,Masayuki; Hoshino,Isamu; Akutsu,Yasunori; Hanari,Naoyuki; Yoneyama,Yasuo; Ikeda,Norimasa; Isozaki,Yuka; Maruyama,Tetsurou; Akanuma,Naoki; Miyazawa,Yukimasa; Matsubara,Hisahiro

doi:10.3892/ijo.2012.1614

miR-203 inhibits the migration and invasion of esophageal squamous cell carcinoma by regulating LASP1

Authors:
- Nobuyoshi Takeshita
- Mikito Mori
- Masayuki Kano
- Isamu Hoshino
- Yasunori Akutsu
- Naoyuki Hanari
- Yasuo Yoneyama
- Norimasa Ikeda
- Yuka Isozaki
- Tetsurou Maruyama
- Naoki Akanuma
- Yukimasa Miyazawa
- Hisahiro Matsubara
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Affiliations: Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chiba, Japan
Published online on: August 30, 2012 https://doi.org/10.3892/ijo.2012.1614
Pages: 1653-1661

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Abstract

The expression of microRNA-203 (miR-203) in esophageal squamous cell carcinoma (ESCC) tissues is remarkably lower than that in non‑ESCC tissues. We investigated how miR-203 could influence the development of ESCC cells. Our analyses revealed that miR-203 inhibited the migration and invasion of ESCC cells. Genome-wide gene expression data and target site inhibition assays showed that miR-203 appears to directly regulate LIM and SH3 protein 1 (LASP1). The knockdown of LASP1 resulted in inhibition of the migration and invasion of ESCC cells. Our results suggest that miR-203 and its target LASP1, may be associated with the progression of ESCC. In clinical ESCC specimens, the expression levels of miR-203, which were lower compared to those in normal tissues, were inversely correlated with the mRNA expression levels of LASP1. Moreover, we found that there was a significant correlation between the expression levels of miR-203 and the relapse‑free survival. The identification of a cancer network regulated by miR-203 could provide new insights into the potential mechanisms of the progression of ESCC.

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