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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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December 2012 Volume 41 Issue 6

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Chemoprevention of pancreatic cancer using solid-lipid nanoparticulate delivery of a novel aspirin, curcumin and sulforaphane drug combination regimen

  • Authors:
    • Dhruvitkumar Sutaria
    • Balagangadhar Karthik Grandhi
    • Arvind Thakkar
    • Jeffrey Wang
    • Sunil Prabhu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmaceutical Sciences, College of Pharmacy, Western University of Health Sciences, Pomona, CA 91766, USA
  • Pages: 2260-2268
    |
    Published online on: September 21, 2012
       https://doi.org/10.3892/ijo.2012.1636
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Abstract

Pancreatic cancer is the fourth largest cause of cancer deaths in the Unites States and the prognosis is grim with <5% survival chances upon diagnosis. The objective of this study was to assess the combined chemopreventive effect of solid lipid nanoparticle (SLN) encapsulated drugs aspirin (ASP), curcumin (CUR) and free sulforaphane (SFN) for the chemoprevention of pancreatic cancer. Experiments were carried out (1) to evaluate the feasibility of encapsulation of these chemopreventive agents within solid lipid systems and (2) to measure the synergistic effects of a combination of ASP with CUR in SLNs mixed with free SFN against cell proliferation and apoptosis in pancreatic cancer cells, MIA PaCa-2 and Panc-1. The SLNs were prepared using a modified solvent evaporation technique and were characterized for particle sizing, encapsulation efficiency and drug release. ASP and CUR SLNs were formulated within the particle size range of 150‑250 nm and were found to have an encapsulation efficiency of 85 and 69%, respectively. Sustained release of drugs over a 96 h period from SLNs was observed. The SLNs were stable over a 3-month storage period at room temperature. Cell viability studies demonstrated that combinations of low doses of ASP SLN (25 µM), CUR SLN (2.5 µM) and free SFN (5 µM) significantly reduced cell viability by 43.6 and 48.49% in MIAPaca-2 and Panc-1 cell lines, respectively. Furthermore, increased apoptosis of 61.3 and 60.37% was found in MIA Paca-2 and Panc-1 cell lines, respectively, in comparison to the individual doses administered. Synergistic effects were demonstrated using MTS and apoptosis assays. Thus, this study successfully demonstrated the feasibility of using a solid lipid nanoparticulate system for the first time to deliver this novel combination chemoprevention regimen, providing valuable evidence for the usability of nanotechnology-based drug regimens towards pancreatic cancer chemoprevention.
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Copy and paste a formatted citation
Spandidos Publications style
Sutaria D, Grandhi BK, Thakkar A, Wang J and Prabhu S: Chemoprevention of pancreatic cancer using solid-lipid nanoparticulate delivery of a novel aspirin, curcumin and sulforaphane drug combination regimen. Int J Oncol 41: 2260-2268, 2012.
APA
Sutaria, D., Grandhi, B.K., Thakkar, A., Wang, J., & Prabhu, S. (2012). Chemoprevention of pancreatic cancer using solid-lipid nanoparticulate delivery of a novel aspirin, curcumin and sulforaphane drug combination regimen. International Journal of Oncology, 41, 2260-2268. https://doi.org/10.3892/ijo.2012.1636
MLA
Sutaria, D., Grandhi, B. K., Thakkar, A., Wang, J., Prabhu, S."Chemoprevention of pancreatic cancer using solid-lipid nanoparticulate delivery of a novel aspirin, curcumin and sulforaphane drug combination regimen". International Journal of Oncology 41.6 (2012): 2260-2268.
Chicago
Sutaria, D., Grandhi, B. K., Thakkar, A., Wang, J., Prabhu, S."Chemoprevention of pancreatic cancer using solid-lipid nanoparticulate delivery of a novel aspirin, curcumin and sulforaphane drug combination regimen". International Journal of Oncology 41, no. 6 (2012): 2260-2268. https://doi.org/10.3892/ijo.2012.1636
Copy and paste a formatted citation
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Spandidos Publications style
Sutaria D, Grandhi BK, Thakkar A, Wang J and Prabhu S: Chemoprevention of pancreatic cancer using solid-lipid nanoparticulate delivery of a novel aspirin, curcumin and sulforaphane drug combination regimen. Int J Oncol 41: 2260-2268, 2012.
APA
Sutaria, D., Grandhi, B.K., Thakkar, A., Wang, J., & Prabhu, S. (2012). Chemoprevention of pancreatic cancer using solid-lipid nanoparticulate delivery of a novel aspirin, curcumin and sulforaphane drug combination regimen. International Journal of Oncology, 41, 2260-2268. https://doi.org/10.3892/ijo.2012.1636
MLA
Sutaria, D., Grandhi, B. K., Thakkar, A., Wang, J., Prabhu, S."Chemoprevention of pancreatic cancer using solid-lipid nanoparticulate delivery of a novel aspirin, curcumin and sulforaphane drug combination regimen". International Journal of Oncology 41.6 (2012): 2260-2268.
Chicago
Sutaria, D., Grandhi, B. K., Thakkar, A., Wang, J., Prabhu, S."Chemoprevention of pancreatic cancer using solid-lipid nanoparticulate delivery of a novel aspirin, curcumin and sulforaphane drug combination regimen". International Journal of Oncology 41, no. 6 (2012): 2260-2268. https://doi.org/10.3892/ijo.2012.1636
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