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Article

The relationship between tissue oxygenation and redox status using magnetic resonance imaging

  • Authors:
    • Fuminori Hyodo
    • Ryan M. Davis
    • Emi Hyodo
    • Shingo Matsumoto
    • Murali C. Krishna
    • James B. Mitchell
  • View Affiliations / Copyright

    Affiliations: Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA
  • Pages: 2103-2108
    |
    Published online on: September 24, 2012
       https://doi.org/10.3892/ijo.2012.1638
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Abstract

The recent development of a bi-modality magnetic resonance imaging/electron paramagnetic resonance imaging (MRI/EPRI) platform has enabled longitudinal monitoring of both tumor oxygenation and redox status in murine cancer models. The current study used this imaging platform to test the hypothesis that a more reducing tumor microenvironment accompanies the development of tumor hypoxia. To test this, the redox status of the tumor was measured using Tempol as a redox‑sensitive MRI contrast agent, and tumor hypoxia was measured with Oxo63, which is an oxygen-sensitive EPRI spin probe. Images were acquired every 1-2 days in mice bearing SCCVII tumors. The median pO2 decreased from 14 mmHg at 7 days after tumor implantation to 7 mmHg at 15 days after implantation. Additionally, the hypoxic fraction, defined as the percentage of the tumor that exhibited a pO2<10 mmHg, increased with tumor size (from 10% at 500 mm3 to 60% at 3,500 mm3). The rate of Tempol reduction increased as a function of tumor volume (0.4 min-1 at 500 mm3 to 1.7 min-1 at 3,500 mm3), suggesting that the tumor microenvironment became more reduced as the tumor grew. The results show that rapid Tempol reduction correlates with decreased tumor oxygenation, and that the Tempol decay rate constant may be a surrogate marker for tumor hypoxia.
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Copy and paste a formatted citation
Spandidos Publications style
Hyodo F, Davis RM, Hyodo E, Matsumoto S, Krishna MC and Mitchell JB: The relationship between tissue oxygenation and redox status using magnetic resonance imaging. Int J Oncol 41: 2103-2108, 2012.
APA
Hyodo, F., Davis, R.M., Hyodo, E., Matsumoto, S., Krishna, M.C., & Mitchell, J.B. (2012). The relationship between tissue oxygenation and redox status using magnetic resonance imaging. International Journal of Oncology, 41, 2103-2108. https://doi.org/10.3892/ijo.2012.1638
MLA
Hyodo, F., Davis, R. M., Hyodo, E., Matsumoto, S., Krishna, M. C., Mitchell, J. B."The relationship between tissue oxygenation and redox status using magnetic resonance imaging". International Journal of Oncology 41.6 (2012): 2103-2108.
Chicago
Hyodo, F., Davis, R. M., Hyodo, E., Matsumoto, S., Krishna, M. C., Mitchell, J. B."The relationship between tissue oxygenation and redox status using magnetic resonance imaging". International Journal of Oncology 41, no. 6 (2012): 2103-2108. https://doi.org/10.3892/ijo.2012.1638
Copy and paste a formatted citation
x
Spandidos Publications style
Hyodo F, Davis RM, Hyodo E, Matsumoto S, Krishna MC and Mitchell JB: The relationship between tissue oxygenation and redox status using magnetic resonance imaging. Int J Oncol 41: 2103-2108, 2012.
APA
Hyodo, F., Davis, R.M., Hyodo, E., Matsumoto, S., Krishna, M.C., & Mitchell, J.B. (2012). The relationship between tissue oxygenation and redox status using magnetic resonance imaging. International Journal of Oncology, 41, 2103-2108. https://doi.org/10.3892/ijo.2012.1638
MLA
Hyodo, F., Davis, R. M., Hyodo, E., Matsumoto, S., Krishna, M. C., Mitchell, J. B."The relationship between tissue oxygenation and redox status using magnetic resonance imaging". International Journal of Oncology 41.6 (2012): 2103-2108.
Chicago
Hyodo, F., Davis, R. M., Hyodo, E., Matsumoto, S., Krishna, M. C., Mitchell, J. B."The relationship between tissue oxygenation and redox status using magnetic resonance imaging". International Journal of Oncology 41, no. 6 (2012): 2103-2108. https://doi.org/10.3892/ijo.2012.1638
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