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December 2012 Volume 41 Issue 6

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Article

Regulation of CXCR4 gene expression in breast cancer cells under diverse stress conditions

  • Authors:
    • Kleopatra Andreou
    • Ramkumar Rajendran
    • Marija Krstic-Demonacos
    • Constantinos Demonacos
  • View Affiliations / Copyright

    Affiliations: School of Pharmacy and Pharmaceutical Sciences, Stopford Building, University of Manchester, Manchester, M13 9PT, UK, Faculty of Life Sciences, Michael Smith Building, University of Manchester, Manchester, M13 9PT, UK
  • Pages: 2253-2259
    |
    Published online on: September 27, 2012
       https://doi.org/10.3892/ijo.2012.1643
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Abstract

Chronic inflammation is a critical component in breast cancer progression. Pro-inflammatory mediators along with growth/survival factors within the tumor microenvironment potentiate the expression of pro-inflammatory cytokines (IL-1, IL-6, TNF-α), chemotactic cytokines and their receptors (CXCR4, CXCL12, CXCL8) and angiogenic factors (VEGF) that often overcome the effect of anti-inflammatory molecules (IL-4, IL-10) thus evading the host's antitumor immunity. Detailed knowledge, therefore, of the regulatory mechanisms determining cytokine levels is essential to understand the pathogenesis of breast cancer. HIF-1α and NF-κB transcription factors are important players for the establishment of a pro-inflammatory and potentially oncogenic environment. HIF-1α is the key mediator of the cellular response to oxygen deprivation and induces the expression of genes involved in survival and angiogenesis within solid hypoxic tumors. The expression of these genes is often modulated by the p53 tumor suppressor protein that induces apoptosis or cell cycle arrest in neoplastic cells. Functional crosstalk between HIF-1α and p53 pathways mediated by modulators shared between the two transcription factors such as SRC-1 and SIRT-1 differentially regulate the expression of distinct subsets of their target genes under variable stress conditions. In an attempt to shed light on the complex regulatory mechanisms involved in cancer-related inflammation, we investigated the role of the two common p53 and HIF-1α co-regulators SRC-1 and SIRT-1, in the expression of the highly potent metastatic chemokine receptor CXCR4. Both SRC-1 and SIRT-1 overexpression in DSFX-treated MCF-7 cells reduced CXCR4 cellular levels implying that both co-regulators are crucial factors in the determination of the metastatic potential of breast cancer cells.
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View References

1. 

Balkwill F and Mantovani A: Inflammation and cancer: back to Virchow? Lancet. 357:539–545. 2001. View Article : Google Scholar : PubMed/NCBI

2. 

de Visser KE, Eichten A and Coussens LM: Paradoxical roles of the immune system during cancer development. Nat Rev Cancer. 6:24–37. 2006.

3. 

Allavena P, Garlanda C, Borrello MG, Sica A and Mantovani A: Pathways connecting inflammation and cancer. Curr Opin Genet Dev. 18:3–10. 2008. View Article : Google Scholar

4. 

Lin WW and Karin M: A cytokine-mediated link between innate immunity, inflammation, and cancer. J Clin Invest. 117:1175–1183. 2007. View Article : Google Scholar : PubMed/NCBI

5. 

Mantovani A, Allavena P, Sica A and Balkwill F: Cancer-related inflammation. Nature. 454:436–444. 2008. View Article : Google Scholar

6. 

Shiao SL, Ganesan AP, Rugo HS and Coussens LM: Immune microenvironments in solid tumours: new targets for therapy. Genes Dev. 25:2559–2572. 2011. View Article : Google Scholar : PubMed/NCBI

7. 

Mantovani A, Sozzani S, Locati M, Allavena P and Sica A: Macrophage polarization: tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes. Trends Immunol. 23:549–555. 2002. View Article : Google Scholar : PubMed/NCBI

8. 

Aggarwal BB, Shishodia S, Sandur SK, Pandey MK and Sethi G: Inflammation and cancer: how hot is the link? Biochem Pharmacol. 72:1605–1621. 2006. View Article : Google Scholar : PubMed/NCBI

9. 

Balkwill F: TNF-alpha in promotion and progression of cancer. Cancer Metastasis Rev. 25:409–416. 2006. View Article : Google Scholar : PubMed/NCBI

10. 

Mocellin S, Marincola FM and Young HA: Interleukin-10 and the immune response against cancer: a counterpoint. J Leukoc Biol. 78:1043–1051. 2005. View Article : Google Scholar : PubMed/NCBI

11. 

Ali S and Lazennec G: Chemokines: novel targets for breast cancer metastasis. Cancer Metastasis Rev. 26:401–420. 2007. View Article : Google Scholar : PubMed/NCBI

12. 

Balkwill F: Chemokine biology in cancer. Semin Immunol. 15:49–55. 2003. View Article : Google Scholar : PubMed/NCBI

13. 

Allavena P, Germano G, Marchesi F and Mantovani A: Chemokines in cancer-related inflammation. Exp Cell Res. 317:664–673. 2011. View Article : Google Scholar : PubMed/NCBI

14. 

Luker KE and Luker GD: Functions of CXCL12 and CXCR4 in breast cancer. Cancer Lett. 238:30–41. 2006. View Article : Google Scholar : PubMed/NCBI

15. 

Balkwill F: The significance of cancer cell expression of the chemokine receptor CXCR4. Semin Cancer Biol. 14:171–179. 2004. View Article : Google Scholar : PubMed/NCBI

16. 

Uygur B and Wu WS: SLUG promotes prostate cancer cell migration and invasion via CXCR4/CXCL12 axis. Mol Cancer. 10:1392011. View Article : Google Scholar : PubMed/NCBI

17. 

Balkwill F: Cancer and the chemokine network. Nat Rev Cancer. 4:540–550. 2004. View Article : Google Scholar

18. 

Denko NC, Fontana LA, Hudson KM, et al: Investigating hypoxic tumor physiology through gene expression patterns. Oncogene. 22:5907–5914. 2003. View Article : Google Scholar : PubMed/NCBI

19. 

Schioppa T, Uranchimeg B, Saccani A, et al: Regulation of the chemokine receptor CXCR4 by hypoxia. J Exp Med. 198:1391–1402. 2003. View Article : Google Scholar : PubMed/NCBI

20. 

Mehta SA, Christopherson KW, Bhat-Nakshatri P, et al: Negative regulation of chemokine receptor CXCR4 by tumor suppressor p53 in breast cancer cells: implications of p53 mutation or isoform expression on breast cancer cell invasion. Oncogene. 26:3329–3337. 2007. View Article : Google Scholar : PubMed/NCBI

21. 

Webster GA and Perkins ND: Transcriptional cross-talk between NF-kappaB and p53. Mol Cell Biol. 19:3485–3495. 1999.PubMed/NCBI

22. 

Xenaki G, Ontikatze T, Rajendran R, et al: PCAF is an HIF-1alpha cofactor that regulates p53 transcriptional activity in hypoxia. Oncogene. 27:5785–5796. 2008. View Article : Google Scholar : PubMed/NCBI

23. 

Blagosklonny MV, An WG, Romanova LY, Trepel J, Fojo T and Neckers L: p53 inhibits hypoxia-inducible factor-stimulated transcription. J Biol Chem. 273:11995–11998. 1998. View Article : Google Scholar : PubMed/NCBI

24. 

An WG, Kanekal M, Simon MC, Maltepe E, Blagosklonny MV and Neckers LM: Stabilization of wild-type p53 by hypoxia-inducible factor 1alpha. Nature. 392:405–408. 1998. View Article : Google Scholar : PubMed/NCBI

25. 

Lee SK, Kim HJ, Kim JW and Lee JW: Steroid receptor coactivator-1 and its family members differentially regulate trans-activation by the tumor suppressor protein p53. Mol Endocrinol. 13:1924–1933. 1999. View Article : Google Scholar

26. 

Ruas JL, Poellinger L and Pereira T: Role of CBP in regulating HIF-1-mediated activation of transcription. J Cell Sci. 118:301–311. 2005. View Article : Google Scholar : PubMed/NCBI

27. 

Rajendran R, Garva R, Krstic-Demonacos M and Demonacos C: Sirtuins: molecular traffic lights in the crossroad of oxidative stress, chromatin remodeling, and transcription. J Biomed Biotechnol. 2011:3682762011. View Article : Google Scholar : PubMed/NCBI

28. 

Xu J, Wu R-C and O’Malley BW: Normal and cancer-related functions of the p160 steroid receptor co-activator (SRC) family. Nat Rev Cancer. 9:615–630. 2009. View Article : Google Scholar : PubMed/NCBI

29. 

Onate SA, Tsai SY, Tsai MJ and O’Malley BW: Sequence and characterization of a coactivator for the steroid hormone receptor superfamily. Science. 270:1354–1357. 1995. View Article : Google Scholar : PubMed/NCBI

30. 

Demonacos C and La Thangue NB: Drug discovery and the p53 family. Prog Cell Cycle Res. 5:375–382. 2003.PubMed/NCBI

31. 

Keimling M and Wiesmuller L: DNA double-strand break repair activities in mammary epithelial cells - influence of endogenous p53 variants. Carcinogenesis. 30:1260–1268. 2009. View Article : Google Scholar : PubMed/NCBI

32. 

Carrero P, Okamoto K, Coumailleau P, O’Brien S, Tanaka H and Poellinger L: Redox-regulated recruitment of the transcriptional coactivators CREB-binding protein and SRC-1 to hypoxia-inducible factor 1alpha. Mol Cell Biol. 20:402–415. 2000. View Article : Google Scholar : PubMed/NCBI

33. 

Lavery DN and Bevan CL: Androgen receptor signalling in prostate cancer: the functional consequences of acetylation. J Biomed Biotechnol. 2011:8621252011. View Article : Google Scholar : PubMed/NCBI

34. 

Zhang Q, Tang X, Lu QY, Zhang ZF, Brown J and Le AD: Resveratrol inhibits hypoxia-induced accumulation of hypoxia-inducible factor-1alpha and VEGF expression in human tongue squamous cell carcinoma and hepatoma cells. Mol Cancer Ther. 4:1465–1474. 2005. View Article : Google Scholar : PubMed/NCBI

35. 

Lim J-H, Lee Y-M, Chun Y-S, Chen J, Kim J-E and Park J-W: Sirtuin 1 modulates cellular responses to hypoxia by deacetylating hypoxia-inducible factor 1α. Mol Cell. 38:864–878. 2010.PubMed/NCBI

36. 

De Nardo D and Latz E: NLRP3 inflammasomes link inflammation and metabolic disease. Trends Immunol. 32:373–379. 2011.PubMed/NCBI

37. 

Strober W and Fuss IJ: Proinflammatory cytokines in the pathogenesis of inflammatory bowel diseases. Gastroenterology. 140:1756–1767. 2011. View Article : Google Scholar : PubMed/NCBI

38. 

Mantovani A, Marchesi F, Porta C, Sica A and Allavena P: Inflammation and cancer: breast cancer as a prototype. Breast. 16(Suppl 2): S27–S33. 2007. View Article : Google Scholar : PubMed/NCBI

39. 

Toi M, Bicknell R and Harris AL: Inhibition of colon and breast carcinoma cell growth by interleukin-4. Cancer Res. 52:275–279. 1992.PubMed/NCBI

40. 

Murdoch C and Lewis CE: Macrophage migration and gene expression in response to tumor hypoxia. Int J Cancer. 117:701–708. 2005. View Article : Google Scholar : PubMed/NCBI

41. 

Hussain SP and Harris CC: Inflammation and cancer: an ancient link with novel potentials. Int J Cancer. 121:2373–2380. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Andreou K, Rajendran R, Krstic-Demonacos M and Demonacos C: Regulation of CXCR4 gene expression in breast cancer cells under diverse stress conditions. Int J Oncol 41: 2253-2259, 2012.
APA
Andreou, K., Rajendran, R., Krstic-Demonacos, M., & Demonacos, C. (2012). Regulation of CXCR4 gene expression in breast cancer cells under diverse stress conditions. International Journal of Oncology, 41, 2253-2259. https://doi.org/10.3892/ijo.2012.1643
MLA
Andreou, K., Rajendran, R., Krstic-Demonacos, M., Demonacos, C."Regulation of CXCR4 gene expression in breast cancer cells under diverse stress conditions". International Journal of Oncology 41.6 (2012): 2253-2259.
Chicago
Andreou, K., Rajendran, R., Krstic-Demonacos, M., Demonacos, C."Regulation of CXCR4 gene expression in breast cancer cells under diverse stress conditions". International Journal of Oncology 41, no. 6 (2012): 2253-2259. https://doi.org/10.3892/ijo.2012.1643
Copy and paste a formatted citation
x
Spandidos Publications style
Andreou K, Rajendran R, Krstic-Demonacos M and Demonacos C: Regulation of CXCR4 gene expression in breast cancer cells under diverse stress conditions. Int J Oncol 41: 2253-2259, 2012.
APA
Andreou, K., Rajendran, R., Krstic-Demonacos, M., & Demonacos, C. (2012). Regulation of CXCR4 gene expression in breast cancer cells under diverse stress conditions. International Journal of Oncology, 41, 2253-2259. https://doi.org/10.3892/ijo.2012.1643
MLA
Andreou, K., Rajendran, R., Krstic-Demonacos, M., Demonacos, C."Regulation of CXCR4 gene expression in breast cancer cells under diverse stress conditions". International Journal of Oncology 41.6 (2012): 2253-2259.
Chicago
Andreou, K., Rajendran, R., Krstic-Demonacos, M., Demonacos, C."Regulation of CXCR4 gene expression in breast cancer cells under diverse stress conditions". International Journal of Oncology 41, no. 6 (2012): 2253-2259. https://doi.org/10.3892/ijo.2012.1643
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