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Article

Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1

  • Authors:
    • Jianguo Chen
    • Yang Chen
    • Qiyang He
  • View Affiliations / Copyright

    Affiliations: Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, P.R. China
  • Pages: 2245-2252
    |
    Published online on: October 16, 2012
       https://doi.org/10.3892/ijo.2012.1668
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Abstract

Bleomycin is a first-line clinically used antitumor antibiotic for effective treatment of certain types of cancer in combination with other antitumor agents. Its action is affected by bleomycin hydrolase (BLH), DNA repair enzymes, membrane transport proteins and other cellular factors. To clarify whether BLH confers the resistance to bleomycin in tumor cells, it is necessary to further investigate the roles of BLH and its combination with other factors such as caveolin-1 in the action of bleomycin. In this study fourteen human cell lines were used for determination of bleomycin action and roles of BLH and caveolin-1. The relationship between action of bleomycin and cellular amount of BLH was detected by the MTT method and western blotting in the human leukemia cell line HL-60, HeLa cervical cancer cells and HaCaT immortalized keratinocyte cells. The sensitivity to bleomycin was increased in HeLa cells after knockdown of BLH mRNA by RNA interference. There is no relationship between caveolin-1 levels and action of bleomycin, although the distribution of the cell cycle was altered in the caveolin-1-knockdown HeLa cells after treatment with bleomycin. In addition, regulation of BLH and caveolin-1 expression in HeLa and HaCaT cells was observed in a concentration-dependent manner after exposure to bleomycin. In conclusion, bleomycin hydrolase is one of the biomarkers for determination of bleomycin action. Although caveolin-1 can respond to bleomycin treatment, it is unrelated to bleomycin sensitivity.
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Copy and paste a formatted citation
Spandidos Publications style
Chen J, Chen Y and He Q: Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1. Int J Oncol 41: 2245-2252, 2012.
APA
Chen, J., Chen, Y., & He, Q. (2012). Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1. International Journal of Oncology, 41, 2245-2252. https://doi.org/10.3892/ijo.2012.1668
MLA
Chen, J., Chen, Y., He, Q."Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1". International Journal of Oncology 41.6 (2012): 2245-2252.
Chicago
Chen, J., Chen, Y., He, Q."Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1". International Journal of Oncology 41, no. 6 (2012): 2245-2252. https://doi.org/10.3892/ijo.2012.1668
Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Chen Y and He Q: Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1. Int J Oncol 41: 2245-2252, 2012.
APA
Chen, J., Chen, Y., & He, Q. (2012). Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1. International Journal of Oncology, 41, 2245-2252. https://doi.org/10.3892/ijo.2012.1668
MLA
Chen, J., Chen, Y., He, Q."Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1". International Journal of Oncology 41.6 (2012): 2245-2252.
Chicago
Chen, J., Chen, Y., He, Q."Action of bleomycin is affected by bleomycin hydrolase but not by caveolin-1". International Journal of Oncology 41, no. 6 (2012): 2245-2252. https://doi.org/10.3892/ijo.2012.1668
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