Open Access

Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells

  • Authors:
    • Zrinka Bulj
    • Serena Duchi
    • Alessandro Bevilacqua
    • Alessandro Gherardi
    • Barbara Dozza
    • Filippo Piccinini
    • Giulia Adalgisa Mariani
    • Enrico Lucarelli
    • Sandro Giannini
    • Davide Donati
    • Sandra Marmiroli
  • View Affiliations

  • Published online on: November 15, 2012     https://doi.org/10.3892/ijo.2012.1700
  • Pages: 118-126
  • Copyright: © Bulj et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for cell migration and invasion. The tests that were implemented revealed that MSC were fairly migratory. Protein kinase B (AKT) was strongly activated at the basal level. Through our analyses we demonstrated that pharmacological inactivation of AKT2 but not AKT1 significantly decreased cell migration and invasion. Although preliminary, collectively our results indicate that AKT2 activation plays a critical role in enabling MSC migration.
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January 2013
Volume 42 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Bulj, Z., Duchi, S., Bevilacqua, A., Gherardi, A., Dozza, B., Piccinini, F. ... Marmiroli, S. (2013). Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells. International Journal of Oncology, 42, 118-126. https://doi.org/10.3892/ijo.2012.1700
MLA
Bulj, Z., Duchi, S., Bevilacqua, A., Gherardi, A., Dozza, B., Piccinini, F., Adalgisa Mariani, G., Lucarelli, E., Giannini, S., Donati, D., Marmiroli, S."Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells". International Journal of Oncology 42.1 (2013): 118-126.
Chicago
Bulj, Z., Duchi, S., Bevilacqua, A., Gherardi, A., Dozza, B., Piccinini, F., Adalgisa Mariani, G., Lucarelli, E., Giannini, S., Donati, D., Marmiroli, S."Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells". International Journal of Oncology 42, no. 1 (2013): 118-126. https://doi.org/10.3892/ijo.2012.1700