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Article

Trichostatin A induces apoptotic cell death of HeLa cells in a Bcl-2 and oxidative stress-dependent manner

  • Authors:
    • Bo Ra You
    • Woo Hyun Park
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Medical School, Research Institute for Endocrine Sciences, Chonbuk National University, JeonJu 561-180, Republic of Korea
  • Pages: 359-366
    |
    Published online on: November 19, 2012
       https://doi.org/10.3892/ijo.2012.1705
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Abstract

Trichostatin A (TSA) as a HDAC inhibitor can regulate many biological properties including apoptosis and cell proliferation in various cancer cells. Here, we evaluated the effect of TSA on the growth and death of HeLa cervical cancer cells in relation to reactive oxygen species (ROS) and glutathione (GSH) levels. Dose- and time-dependent growth inhibition was observed in HeLa cells with an IC50 of approximately 20 nM at 72 h. This agent also induced apoptotic cell death, as evidenced by annexin V-FITC staining cells, caspase-3 activation and the loss of mitochondrial membrane potential (MMP; ∆ψm). In addition, the administration of Bcl-2 siRNA intensified TSA-induced HeLa cell death. All of the tested caspase inhibitors significantly rescued some cells from TSA-induced HeLa cell death. TSA increased O2•- level and induced GSH depletion in HeLa cells. Caspase inhibitors significantly attenuated O2•- level and GSH depletion in TSA-treated HeLa cells. In addition, N-acetyl cysteine (NAC; a well known antioxidant) significantly prevented cell death and GSH depletion in TSA-treated HeLa cells via decreasing O2•- level. In conclusion, TSA inhibited the growth of HeLa cells via Bcl-2-mediated apoptosis, which was closely related to O2•- and GSH content levels.
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Copy and paste a formatted citation
Spandidos Publications style
You BR and Park WH: Trichostatin A induces apoptotic cell death of HeLa cells in a Bcl-2 and oxidative stress-dependent manner. Int J Oncol 42: 359-366, 2013.
APA
You, B.R., & Park, W.H. (2013). Trichostatin A induces apoptotic cell death of HeLa cells in a Bcl-2 and oxidative stress-dependent manner. International Journal of Oncology, 42, 359-366. https://doi.org/10.3892/ijo.2012.1705
MLA
You, B. R., Park, W. H."Trichostatin A induces apoptotic cell death of HeLa cells in a Bcl-2 and oxidative stress-dependent manner". International Journal of Oncology 42.1 (2013): 359-366.
Chicago
You, B. R., Park, W. H."Trichostatin A induces apoptotic cell death of HeLa cells in a Bcl-2 and oxidative stress-dependent manner". International Journal of Oncology 42, no. 1 (2013): 359-366. https://doi.org/10.3892/ijo.2012.1705
Copy and paste a formatted citation
x
Spandidos Publications style
You BR and Park WH: Trichostatin A induces apoptotic cell death of HeLa cells in a Bcl-2 and oxidative stress-dependent manner. Int J Oncol 42: 359-366, 2013.
APA
You, B.R., & Park, W.H. (2013). Trichostatin A induces apoptotic cell death of HeLa cells in a Bcl-2 and oxidative stress-dependent manner. International Journal of Oncology, 42, 359-366. https://doi.org/10.3892/ijo.2012.1705
MLA
You, B. R., Park, W. H."Trichostatin A induces apoptotic cell death of HeLa cells in a Bcl-2 and oxidative stress-dependent manner". International Journal of Oncology 42.1 (2013): 359-366.
Chicago
You, B. R., Park, W. H."Trichostatin A induces apoptotic cell death of HeLa cells in a Bcl-2 and oxidative stress-dependent manner". International Journal of Oncology 42, no. 1 (2013): 359-366. https://doi.org/10.3892/ijo.2012.1705
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