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Ascorbate supplementation inhibits growth and metastasis of B16FO melanoma and 4T1 breast cancer cells in vitamin C-deficient mice

  • Authors:
    • John Cha
    • M. Waheed Roomi
    • Vadim Ivanov
    • Tatiana Kalinovsky
    • Aleksandra Niedzwiecki
    • Matthias Rath
  • View Affiliations / Copyright

    Affiliations: Dr Rath Research Institute, 1260 Memorex Drive, Santa Clara, CA 95050, USA
    Copyright: © Cha et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 55-64
    |
    Published online on: November 21, 2012
       https://doi.org/10.3892/ijo.2012.1712
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Abstract

Degradation of the extracellular matrix (ECM) plays a critical role in the formation of tumors and metastasis and has been found to correlate with the aggressiveness of tumor growth and invasiveness of cancer. Ascorbic acid, which is known to be essential for the structural integrity of the intercellular matrix, is not produced by humans and must be obtained from the diet. Cancer patients have been shown to have very low reserves of ascorbic acid. Our main objective was to determine the effect of ascorbate supplementation on metastasis, tumor growth and tumor immunohistochemistry in mice unable to synthesize ascorbic acid [gulonolactone oxidase (gulo) knockout (KO)] when challenged with B16FO melanoma or 4T1 breast cancer cells. Gulo KO female mice 36-38 weeks of age were deprived of or maintained on ascorbate in food and water for 4 weeks prior to and 2 weeks post intraperitoneal (IP) injection of 5x105 B16FO murine melanoma cells or to injection of 5x105 4T1 breast cancer cells into the mammary pad of mice. Ascorbate-supplemented gulo KO mice injected with B16FO melanoma cells demonstrated significant reduction (by 71%, p=0.005) in tumor metastasis compared to gulo KO mice on the control diet. The mean tumor weight in ascorbate supplemented mice injected with 4T1 cells was reduced by 28% compared to tumor weight in scorbutic mice. Scorbutic tumors demonstrated large dark cores, associated with increased necrotic areas and breaches to the tumor surface, apoptosis and matrix metalloproteinase-9 (MMP-9), and weak, disorganized or missing collagen I tumor capsule. In contrast, the ascorbate-supplemented group tumors had smaller fainter colored cores and confined areas of necrosis/apoptosis with no breaches from the core to the outside of the tumor and a robust collagen I tumor capsule. In both studies, ascorbate supplementation of gulo KO mice resulted in profoundly decreased serum inflammatory cytokine interleukin (IL)-6 (99% decrease, p=0.01 in the B16F0 study and 85% decrease, p=0.08 in the 4T1 study) compared to the levels in gulo KO mice deprived of ascorbate. In the B16FO study, ascorbate supplementation of gulo KO mice resulted in profoundly decreased serum VEGF (98% decrease, p=0.019 than in the scorbutic gulo KO mice). As expected, mean serum ascorbate level in ascorbate-restricted mice was 2% (p<0.001) of the mean ascorbate levels in supplemented mice. In conclusion, ascorbate supplementation hinders metastasis, tumor growth and inflammatory cytokine secretion as well as enhanced encapsulation of tumors elicited by melanoma and breast cancer cell challenge in gulo KO mice.
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View References

1. 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

2. 

Breastcancer.org: U.S. Breast Cancer Statistics: http://www.breastcancer.org/symptoms/understand_bc/statistics.jsp (accessed December 21, 2011).

3. 

Ali SM, Harvey HA and Lipton A: Metastatic breast cancer: overview of treatment. Clin Orthop. 414(Suppl): 132–137. 2003. View Article : Google Scholar

4. 

Fidler IJ: Molecular biology of cancer: invasion and metastasis. Cancer: Principles and Practice of Oncology. De Vita VT, Hellman S and Rosenberg SA: 5th edition. Lippincott-Raven; Philadelphia, PA: pp. 135–152. 1997

5. 

Rath M and Pauling L: Plasmin-induced proteolysis and the role of apoprotein(a), lysine and synthetic analogs. Orthomol Med. 7:17–23. 1992.

6. 

Nunez MC, De Apodaca O and Ruiz AL: Ascorbic acid in the plasma and blood cells of women with breast cancer. The effect of consumption of food with an elevated content of this vitamin. Nutr Hosp. 10:368–372. 1995.PubMed/NCBI

7. 

Anthony HM and Schorah CJ: Severe hypovitaminosis C in lung-cancer patients: the utilization of vitamin C in surgical repair and lymphocyte-related host resistance. Br J Cancer. 46:354–367. 1982. View Article : Google Scholar : PubMed/NCBI

8. 

Pulaski BA and Ostrand-Rosenberg S: Mouse 4T1 breast tumor model. Curr Protoc Immunol Chapter. 20:Unit 20.2.2001. View Article : Google Scholar

9. 

Tao K, Fang M, Alroy J and Sahagian GG: Imagable 4T1 model for the study of late stage breast cancer. BMC Cancer. 8:2282008. View Article : Google Scholar : PubMed/NCBI

10. 

Hart IR and Fidler IJ: Role of organ selectivity in the determination of metastatic patterns of B16 melanoma. Cancer Res. 40:2281–2297. 1980.PubMed/NCBI

11. 

Mayland CR, Bennett MI and Allan K: Vitamin C deficiency in cancer patients. Palliat Med. 19:17–20. 2005. View Article : Google Scholar : PubMed/NCBI

12. 

Kuiper C, Molenaar IG, Dachs GU, Currie MJ, Sykes PH and Vissers MC: Low ascorbate levels are associated with increased hypoxia-inducible factor-1 activity and an aggressive tumor phenotype in endometrial cancer. Cancer Res. 70:5749–5758. 2010. View Article : Google Scholar : PubMed/NCBI

13. 

Cameron E, Pauling L and Leibovitz B: Ascorbic acid and cancer: a review. Cancer Res. 39:663–681. 1979.

14. 

Stetler-Stevenson WG, Hewitt R and Corcoran M: Matrix metalloproteinases and tumor invasion from correlation and causality to the clinic. Semin Cancer Biol. 7:147–154. 1996. View Article : Google Scholar : PubMed/NCBI

15. 

Bachmeier BE, Nerlich AG, Lichtinghagen R and Sommerhoff CP: Matrix metalloproteinases (MMPs) in breast cancer cell lines of different tumorigenicity. Anticancer Res. 6A:3821–3828. 2001.PubMed/NCBI

16. 

Pellikainen JM, Ropponen KM, Kataja VV, Kellokoski JK, Eskelinen MJ and Kosma VM: Expression of matrix metalloproteinase (MMP)-2 and MMP-9 in breast cancer with a special reference to activator protein-2, HER-2, and prognosis. Clin Cancer Res. 10:7621–7628. 2004. View Article : Google Scholar : PubMed/NCBI

17. 

Nikkola J, Vihinen P, Vuorista MS, Kellokumpu-Lehtinen P, Kähäri VM and Pyrhönen S: High serum levels of matrix metalloproteinase-9 and matrix metallopreoteinase-1 are associated with rapid progression in patients with metastatic melanoma. Clin Cancer Res. 11:5158–5166. 2005. View Article : Google Scholar : PubMed/NCBI

18. 

Bonfil RD, Bustuabad OD, Ruggiero RA, Meiss RP and Pasqualini CD: Tumor necrosis can facilitate the appearance of metastases. Clin Exp Metastasis. 6:121–129. 1988. View Article : Google Scholar : PubMed/NCBI

19. 

Bonfil RD, Medina PA, Gómez DE, Farías E, Lazarowski A, Lucero Gritti MF, Meiss RP and Bustuabad OD: Expression of gelatinase/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion. Clin Exp Metastasis. 10:211–220. 1992. View Article : Google Scholar : PubMed/NCBI

20. 

Argilés JM, Busquets S, Toledo M and López-Soriano FJ: The role of cytokines in cancer cachexia. Curr Opin Support Palliat Care. 3:263–268. 2009.

21. 

Deans C and Wigmore SJ: Systemic inflammation, cachexia and prognosis in patients with cancer. Curr Opin Clin Nutr Metab Care. 8:265–269. 2005. View Article : Google Scholar : PubMed/NCBI

22. 

Musselman D, Miller A, Porter M, Manatunga A, Gao F, Penna S, Pearce BD, Landry J, Glover S, McDaniel JS and Nemeroff CB: Higher than normal plasma interleukin-6 concentrations in cancer patients with depression: Preliminary findings. Am J Psychiatry. 158:1252–1257. 2001. View Article : Google Scholar : PubMed/NCBI

23. 

Bower J, Ganz P, Desmond K, Rowland J, Meyerowitz BE and Belin TR: Fatigue in breast cancer survivors: occurrence, correlates and impact on quality of life. J Clin Oncol. 18:743–753. 2000.PubMed/NCBI

24. 

Lyon DE, McCain NL, Walter J and Schubert C: Cytokine comparisons between women with breast cancer and women with a negative breast biopsy. Nurs Res. 57:51–58. 2008. View Article : Google Scholar : PubMed/NCBI

25. 

Yurkovetsy ZR, Kirkwood JM, Edington HD, Marragoni AM, Velikokhatnaya L, Winans MT, Gorelik E and Lokshin AE: Multiplex analysis of serum cytokines in melanoma patients treated with interferon-α2b. Clin Cancer Res. 13:2422–2428. 2007.

26. 

Lazar-Molnar E, Hegyesi H, Toth S and Falus A: Autocrine and paracrine regulation by cytokines and growth factors in melanoma. Cytokine. 12:547–554. 2000. View Article : Google Scholar : PubMed/NCBI

27. 

Leek R and Harris A: Tumor-associated macrophages in breast cancer. J Mammary Gland Biol Neoplasia. 7:177–189. 2002. View Article : Google Scholar : PubMed/NCBI

28. 

Bachelot T, Ray-Coquard I, Menetrier-Caux C, Rashtkha M, Duc A and Blay JY: Prognostic value of serum levels of interleukin 6 and of serum and plasma levels of vascular endothelial growth factor in hormone-refractory metastatic breast cancer patients. Br J Cancer. 88:1721–1726. 2003. View Article : Google Scholar

29. 

Salgado R, Junius S, Benoy I, Van Dam P, Vermeulen P, Van Marck E, Huget P and Dirix LY: Circulating interleukin-6 predicts survival in patients with metastatic breast cancer. Int J Cancer. 103:642–646. 2003. View Article : Google Scholar : PubMed/NCBI

30. 

Zhang GJ and Adachi I: Serum interleukin-6 levels correlate to tumor progression and prognosis in metastatic breast carcinoma. Anticancer Res. 19:1427–1432. 1999.PubMed/NCBI

31. 

Mouawad R, Benhammouda A, Rixe O, Antoine EC, Borel C, Weil M, Khayat D and Soubrane C: Endogenous interleukin 6 levels in patients with metastatic malignant melanoma: correlation with tumor burden. Clin Cancer Res. 2:1405–1409. 1996.PubMed/NCBI

32. 

Mouawad R, Rixe O, Meric JB, Khayat D and Soubrane C: Serum interleukin 6 concentration as predictive factor of time to progression in metastatic malignant melanoma patients treated by biochemotherapy: a retrospective study. Cytokines Cell Mol Ther. 7:151–156. 2002. View Article : Google Scholar

33. 

Tawara K, Oxford JT and Jorcyk CL: Clinical significance of interleukin (IL)-6 in cancer metastasis to bone: potential of anti-IL-6 therapies. Cancer Manag Res. 3:177–189. 2011.PubMed/NCBI

34. 

Maeda N, Hagihara H, Nakata Y, Hiller S, Wilder J and Reddick R: Aortic wall damage in mice unable to synthesize ascorbic acid. Proc Natl Acad Sci USA. 97:841–846. 2000. View Article : Google Scholar : PubMed/NCBI

35. 

Harakeh S, Diab-Assaf M, Khalife JC, Abu-El-Ardat KA, Baydoun E, Niedzwiecki A, Marwan E, El-Sabban ME and Rath M: Ascorbic acid induces apoptosis in adult T-cell leukemia. Anticancer Res. 27:289–298. 2007.PubMed/NCBI

36. 

Gravenkamp C, Sypniewska R, Gauntt S, Tarango M, Price P and Reddick R: Behavior of metastatic and nonmetastatic breast tumors in old mice. Exp Biol Med. 229:665–675. 2004.PubMed/NCBI

37. 

Cha J, Roomi MW, Ivanov V, Kalinovsky T, Niedzwiecki A and Rath M: Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice. Exp Oncol. 33:226–230. 2011.PubMed/NCBI

38. 

McCormick WJ: Cancer: a collagen disease, secondary to nutritional deficiency? Arch Pediatr. 76:166–171. 1959.PubMed/NCBI

39. 

Ichim TE, Minev B, Braciak T, Luna B, Hunninghake R, Mikirova NA, Jackson JA, Gonzalez MJ, Miranda-Massari JR, Alexandrescu DT, Dasunu CA, Bogin V, Ancans J, Stvens RB, Markosian B, Koropatnick J, Chen CS and Riordan NH: Intravenous ascorbic acid to prevent and treat cancer-associated sepsis? J Transl Med. 9:25–38. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cha J, Roomi MW, Ivanov V, Kalinovsky T, Niedzwiecki A and Rath M: Ascorbate supplementation inhibits growth and metastasis of B16FO melanoma and 4T1 breast cancer cells in vitamin C-deficient mice. Int J Oncol 42: 55-64, 2013.
APA
Cha, J., Roomi, M.W., Ivanov, V., Kalinovsky, T., Niedzwiecki, A., & Rath, M. (2013). Ascorbate supplementation inhibits growth and metastasis of B16FO melanoma and 4T1 breast cancer cells in vitamin C-deficient mice. International Journal of Oncology, 42, 55-64. https://doi.org/10.3892/ijo.2012.1712
MLA
Cha, J., Roomi, M. W., Ivanov, V., Kalinovsky, T., Niedzwiecki, A., Rath, M."Ascorbate supplementation inhibits growth and metastasis of B16FO melanoma and 4T1 breast cancer cells in vitamin C-deficient mice". International Journal of Oncology 42.1 (2013): 55-64.
Chicago
Cha, J., Roomi, M. W., Ivanov, V., Kalinovsky, T., Niedzwiecki, A., Rath, M."Ascorbate supplementation inhibits growth and metastasis of B16FO melanoma and 4T1 breast cancer cells in vitamin C-deficient mice". International Journal of Oncology 42, no. 1 (2013): 55-64. https://doi.org/10.3892/ijo.2012.1712
Copy and paste a formatted citation
x
Spandidos Publications style
Cha J, Roomi MW, Ivanov V, Kalinovsky T, Niedzwiecki A and Rath M: Ascorbate supplementation inhibits growth and metastasis of B16FO melanoma and 4T1 breast cancer cells in vitamin C-deficient mice. Int J Oncol 42: 55-64, 2013.
APA
Cha, J., Roomi, M.W., Ivanov, V., Kalinovsky, T., Niedzwiecki, A., & Rath, M. (2013). Ascorbate supplementation inhibits growth and metastasis of B16FO melanoma and 4T1 breast cancer cells in vitamin C-deficient mice. International Journal of Oncology, 42, 55-64. https://doi.org/10.3892/ijo.2012.1712
MLA
Cha, J., Roomi, M. W., Ivanov, V., Kalinovsky, T., Niedzwiecki, A., Rath, M."Ascorbate supplementation inhibits growth and metastasis of B16FO melanoma and 4T1 breast cancer cells in vitamin C-deficient mice". International Journal of Oncology 42.1 (2013): 55-64.
Chicago
Cha, J., Roomi, M. W., Ivanov, V., Kalinovsky, T., Niedzwiecki, A., Rath, M."Ascorbate supplementation inhibits growth and metastasis of B16FO melanoma and 4T1 breast cancer cells in vitamin C-deficient mice". International Journal of Oncology 42, no. 1 (2013): 55-64. https://doi.org/10.3892/ijo.2012.1712
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