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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

miRNA expression profile in human osteosarcoma: Role of miR-1 and miR-133b in proliferation and cell cycle control

  • Authors:
    • Chiara Novello
    • Laura Pazzaglia
    • Chiara Cingolani
    • Amalia Conti
    • Irene Quattrini
    • Maria Cristina Manara
    • Mauro Tognon
    • Piero Picci
    • Maria Serena Benassi
  • View Affiliations / Copyright

    Affiliations: Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, I-40136 Bologna, Italy, Department of Morphology and Embryology, Section of Cell Biology and Molecular Genetics, School of Medicine and Center of Biotechnology, University of Ferrara, I-44100 Ferrara, Italy
  • Pages: 667-675
    |
    Published online on: November 28, 2012
       https://doi.org/10.3892/ijo.2012.1717
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Abstract

miRNA profile deregulation affecting downstream signaling pathways activates endpoints that represent potential biomarkers for prognosis and treatment of tumor patients. In the past 20 years conventional therapy for osteosarcoma (OS) reached a survival plateau, highlighting the need for new therapeutic approaches. In this study, microarray unsupervised and supervised analysis identified, respectively, 100 and 40 differentially expressed miRNAs in OS samples with different grades of malignancy compared to normal bone. When analyzing low-grade and high-grade OS by unsupervised analysis, 12 miRNAs were found to be differentially expressed. Real‑time PCR performed on a larger series of OS confirmed a significant lower expression of miR-1, miR‑133b and miR-378* in tumors with respect to control, also showing lower mRNA levels in 31 high-grade OS than in 25 low-grade and in metastatic versus non‑metastatic patients. We demonstrated that miR-1 and miR133b were downregulated in OS cell lines compared to normal osteoblasts. Secondly, by transfection with miRNA precursor molecules, we demonstrated that the ectopic expression of miR-1 and miR-133b in U2-OS cell lines significantly reduced cell proliferation and MET protein expression and negatively regulated cell invasiveness and motility in a short-term assay. Cell cycle distribution revealed block in G1 and delay of cell cycle progression associated with increased apoptosis in miR-1- and miR‑133b‑transfected cells, respectively. Our data assessed specific miRNA profiling deregulation in OS clinical samples and suggest that the expression of miR-1 and miR-133b may control cell proliferation and cell cycle through MET protein expression modulation.
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Copy and paste a formatted citation
Spandidos Publications style
Novello C, Pazzaglia L, Cingolani C, Conti A, Quattrini I, Manara MC, Tognon M, Picci P and Benassi MS: miRNA expression profile in human osteosarcoma: Role of miR-1 and miR-133b in proliferation and cell cycle control. Int J Oncol 42: 667-675, 2013.
APA
Novello, C., Pazzaglia, L., Cingolani, C., Conti, A., Quattrini, I., Manara, M.C. ... Benassi, M.S. (2013). miRNA expression profile in human osteosarcoma: Role of miR-1 and miR-133b in proliferation and cell cycle control. International Journal of Oncology, 42, 667-675. https://doi.org/10.3892/ijo.2012.1717
MLA
Novello, C., Pazzaglia, L., Cingolani, C., Conti, A., Quattrini, I., Manara, M. C., Tognon, M., Picci, P., Benassi, M. S."miRNA expression profile in human osteosarcoma: Role of miR-1 and miR-133b in proliferation and cell cycle control". International Journal of Oncology 42.2 (2013): 667-675.
Chicago
Novello, C., Pazzaglia, L., Cingolani, C., Conti, A., Quattrini, I., Manara, M. C., Tognon, M., Picci, P., Benassi, M. S."miRNA expression profile in human osteosarcoma: Role of miR-1 and miR-133b in proliferation and cell cycle control". International Journal of Oncology 42, no. 2 (2013): 667-675. https://doi.org/10.3892/ijo.2012.1717
Copy and paste a formatted citation
x
Spandidos Publications style
Novello C, Pazzaglia L, Cingolani C, Conti A, Quattrini I, Manara MC, Tognon M, Picci P and Benassi MS: miRNA expression profile in human osteosarcoma: Role of miR-1 and miR-133b in proliferation and cell cycle control. Int J Oncol 42: 667-675, 2013.
APA
Novello, C., Pazzaglia, L., Cingolani, C., Conti, A., Quattrini, I., Manara, M.C. ... Benassi, M.S. (2013). miRNA expression profile in human osteosarcoma: Role of miR-1 and miR-133b in proliferation and cell cycle control. International Journal of Oncology, 42, 667-675. https://doi.org/10.3892/ijo.2012.1717
MLA
Novello, C., Pazzaglia, L., Cingolani, C., Conti, A., Quattrini, I., Manara, M. C., Tognon, M., Picci, P., Benassi, M. S."miRNA expression profile in human osteosarcoma: Role of miR-1 and miR-133b in proliferation and cell cycle control". International Journal of Oncology 42.2 (2013): 667-675.
Chicago
Novello, C., Pazzaglia, L., Cingolani, C., Conti, A., Quattrini, I., Manara, M. C., Tognon, M., Picci, P., Benassi, M. S."miRNA expression profile in human osteosarcoma: Role of miR-1 and miR-133b in proliferation and cell cycle control". International Journal of Oncology 42, no. 2 (2013): 667-675. https://doi.org/10.3892/ijo.2012.1717
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