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Article

Apoptosis induction of human prostate carcinoma cells by cordycepin through reactive oxygen species‑mediated mitochondrial death pathway

  • Authors:
    • Hye Hyeon Lee
    • Cheol Park
    • Jin-Woo Jeong
    • Min Jeong Kim
    • Min Jeong Seo
    • Byoung Won Kang
    • Jeong Uck Park
    • Gi-Young Kim
    • Byung Tae Choi
    • Yung Hyun Choi
    • Yong Kee Jeong
  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology, Dong-A University, Busan 604-714, Republic of Korea, Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan 614-052, Republic of Korea, Medi-Farm Industrialization Research Center, Dong-A University, Busan 604-714, Republic of Korea, Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea, Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University, Gyeongnam 626-870, Republic of Korea
  • Pages: 1036-1044
    |
    Published online on: January 4, 2013
       https://doi.org/10.3892/ijo.2013.1762
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Abstract

Cordycepin is the main functional component of Cordyceps militaris, which has been widely used in oriental traditional medicine. This compound has been shown to possess many pharmacological properties, such as enhancing the body's immune function, and anti-inflammatory, anti-aging and anticancer effects. In the present study, we investigated the apoptotic effects of cordycepin in human prostate carcinoma cells. We found that treatment with cordycepin significantly inhibited cell growth by inducing apoptosis in PC-3 cells. Apoptosis induction of PC-3 cells by cordycepin showed correlation with proteolytic activation of caspase-3 and -9, but not caspase-8, and concomitant degradation of poly (ADP-ribose) polymerases, collapse of the mitochondrial membrane potential (MMP). In addition, cordycepin treatment resulted in an increase of the Bax/Bcl-2 (or Bcl-xL) ratio, downregulation of inhibitor of apoptosis protein (IAP) family members, Bax conformational changes, and release of cytochrome c from the mitochondria to the cytosol. The cordycepin-induced apoptosis was also associated with the generation of intracellular reactive oxygen species (ROS). However, the quenching of ROS generation with antioxidant N-acetyl-L-cysteine conferred significant protection against cordycepin-elicited ROS generation, disruption of the MMP, modulation of Bcl-2 and IAP family proteins, caspase-3 and -9 activation and apoptosis. This indicates that the cellular ROS generation plays a pivotal role in the initiation of cordycepin-triggered apoptotic death. Collectively, our findings suggest that cordycepin is a potent inducer of apoptosis of prostate cancer cells via a mitochondrial-mediated intrinsic pathway and that this agent may be of value in the development of a potential therapeutic candidate for both the prevention and treatment of cancer.
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1 

Jemal A, Ward E and Thun M: Declining death rates reflect progress against cancer. PLoS One. 5:e9584–e9591. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Sciarra A, Salciccia S and Panebianco V: Proton spectroscopic and dynamic contrast-enhanced magnetic resonance: a modern approach in prostate cancer imaging. Eur Urol. 54:485–488. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Crawford ED: Epidemiology of prostate cancer. Urology. 62:3–12. 2003. View Article : Google Scholar

4 

Zhang L, Yang BX, Zhang HT, Wang JG, Wang HL and Zhao XJ: Prostate cancer: an emerging threat to the health of aging men in Asia. Asian J Androl. 13:574–578. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Xia SJ, Cui D and Jiang Q: An overview of prostate diseases and their characteristics specific to Asian men. Asian J Androl. 14:458–464. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Neto CC, Amoroso JW and Liberty AM: Anticancer activities of cranberry phytochemicals: an update. Mol Nutr Food Res. 52:S18–S27. 2008.PubMed/NCBI

7 

Kaur M, Pop M, Shi D, Brignone C and Grossman SR: hHR23B is required for genotoxic-specific activation of p53 and apoptosis. Oncogene. 26:1231–1237. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Igney FH and Krammer PH: Immune escape of tumors: apoptosis resistance and tumor counterattack. J Leukoc Biol. 71:907–920. 2002.PubMed/NCBI

9 

Hu W and Kavanagh JJ: Anticancer therapy targeting the apoptotic pathway. Lancet Oncol. 4:721–729. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Pathak N and Khandelwal S: Role of oxidative stress and apoptosis in cadmium induced thymic atrophy and splenomegaly in mice. Toxicol Lett. 169:95–108. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Chatterjee S, Kundu S, Bhattacharyya A, Hartinger CG and Dyson PJ: The ruthenium(II)-arene compound RAPTA-C induces apoptosis in EAC cells through mitochondrial and p53-JNK pathways. J Biol Inorg Chem. 13:1149–1155. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Huppertz B, Kadyrov M and Kingdom JC: Apoptosis and its role in the trophoblast. Am J Obstet Gynecol. 195:29–39. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Zhou H, Liu X, Liu L, Yang Z, Zhang S, Tang M, Tang Y, Dong Q and Hu R: Oxidative stress and apoptosis of human brain microvascular endothelial cells induced by free fatty acids. J Int Med Res. 37:1897–1903. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Wang MF, Liao YF, Hung YC, Lin CL, Hour TC, Lue KH, Hung HC and Liu GY: Hydroxydibenzoylmethane induces apoptosis through repressing ornithine decarboxylase in human promyelocytic leukemia HL-60 cells. Exp Mol Med. 43:189–196. 2011. View Article : Google Scholar

15 

Cunningham KG, Manson W, Spring FS and Hutchinson SA: Cordycepin, a metabolic product isolated from cultures of Cordyceps militaris. Nature. 166:9491950. View Article : Google Scholar : PubMed/NCBI

16 

Paterson RR: Cordyceps: a traditional Chinese medicine and another fungal therapeutic biofactory? Phytochemistry. 69:1469–1495. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Horowitz B, Goldfinger BA and Marmur J: Effect of cordycepin triphosphate on the nuclear DNA-dependent RNA polymerases and poly(A) polymerase from the yeast, Saccharomyces cerevisiae. Arch Biochem Biophys. 172:143–148. 1976. View Article : Google Scholar : PubMed/NCBI

18 

Müller WE, Seibert G, Beyer R, Breter HJ, Maidhof A and Zahn RK: Effect of cordycepin on nucleic acid metabolism in L5178Y cells and on nucleic acid-synthesizing enzyme systems. Cancer Res. 37:3824–3833. 1977.

19 

Foss FM: Combination therapy with purine nucleoside analogs. Oncology. 14:31–35. 2000.PubMed/NCBI

20 

Nakamura K, Yoshikawa N, Yamaguchi Y, Kagota S, Shinozuka K and Kunitomo M: Antitumor effect of cordycepin (3′-deoxyadenosine) on mouse melanoma and lung carcinoma cells involves adenosine A3 receptor stimulation. Anticancer Res. 26:43–47. 2006.

21 

Sugar AM and McCaffrey RP: Antifungal activity of 3′-deoxyadenosine (cordycepin). Antimicrob Agents Chemother. 42:1424–1427. 1998.

22 

Ahn YJ, Park SJ, Lee SG, Shin SC and Choi DH: Cordycepin: selective growth inhibitor derived from liquid culture of Cordyceps militaris against Clostridium spp. J Agric Food Chem. 48:2744–2748. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Kim HG, Shrestha B, Lim SY, Yoon DH, Chang WC, Shin DJ, Han SK, Park SM, Park JH, Park HI, Sung JM, Jang Y, Chung N, Hwang KC and Kim TW: Cordycepin inhibits lipopolysaccharide-induced inflammation by the suppression of NF-kappaB through Akt and p38 inhibition in RAW 264.7 macrophage cells. Eur J Pharmacol. 545:192–199. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Jeong JW, Jin CY, Kim GY, Lee JD, Park C, Kim GD, Kim WJ, Jung WK, Seo SK, Choi IW and Choi YH: Anti-inflammatory effects of cordycepin via suppression of inflammatory mediators in BV2 microglial cells. Int Immunopharmacol. 10:1580–1586. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Zhou X, Gong Z, Su Y, Lin J and Tang K: Cordyceps fungi: natural products, pharmacological functions and developmental products. J Pharm Pharmacol. 61:279–291. 2009. View Article : Google Scholar

26 

Lee K, Lee MH, Kang YW, Rhee KJ, Kim TU and Kim YS: Parkin induces apoptotic cell death in TNF-α-treated cervical cancer cells. BMB Rep. 45:526–531. 2012.PubMed/NCBI

27 

Kim IH, Kim SW, Kim SH, Lee SO, Lee ST, Kim DG, Lee MJ and Park WH: Parthenolide-induced apoptosis of hepatic stellate cells and anti-fibrotic effects in an in vivo rat model. Exp Mol Med. 44:448–456. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Wang X, Chen S, Ma G, Ye M and Lu G: Involvement of proinflammatory factors, apoptosis, caspase-3 activation and Ca2+ disturbance in microglia activation-mediated dopaminergic cell degeneration. Mech Ageing Dev. 126:1241–1254. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Chang HY and Yang X: Proteases for cell suicide: functions and regulation of caspases. Microbiol Mol Biol Rev. 64:821–846. 2000. View Article : Google Scholar : PubMed/NCBI

30 

Jin Z and El-Deiry WS: Overview of cell death signaling pathways. Cancer Biol Ther. 4:139–163. 2005.PubMed/NCBI

31 

Deveraux QL, Roy N, Stennicke HR, Van Arsdale T, Zhou Q, Srinivasula SM, Alnemri ES, Salvesen GS and Reed JC: IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J. 17:2215–2223. 1998. View Article : Google Scholar : PubMed/NCBI

32 

Borner C: The Bcl-2 protein family: sensors and checkpoints for life-or-death decisions. Mol Immunol. 39:615–647. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Griffiths GJ, Dubrez L, Morgan CP, Jones NA, Whitehouse J, Corfe BM, Dive C and Hickman JA: Cell damage-induced conformational changes of the pro-apoptotic protein Bak in vivo precede the onset of apoptosis. J Cell Biol. 144:903–914. 1999. View Article : Google Scholar : PubMed/NCBI

34 

Wolter KG, Hsu YT, Smith CL, Nechushtan A, Xi XG and Youle RJ: Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol. 139:1281–1292. 1997. View Article : Google Scholar : PubMed/NCBI

35 

Bruce-Keller AJ, Begley JG, Fu W, Butterfield DA, Bredesen DE, Hutchins JB, Hensley K and Mattson MP: Bcl-2 protects isolated plasma and mitochondrial membranes against lipid peroxidation induced by hydrogen peroxide and amyloid beta-peptide. J Neurochem. 70:31–39. 1998. View Article : Google Scholar

36 

Fiers W, Beyaert R, Declercq W and Vandenabeele P: More than one way to die: apoptosis, necrosis and reactive oxygen damage. Oncogene. 18:7719–7730. 1999. View Article : Google Scholar : PubMed/NCBI

37 

Fleury C, Mignotte B and Vayssière JL: Mitochondrial reactive oxygen species in cell death signaling. Biochimie. 84:131–141. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lee HH, Park C, Jeong J, Kim MJ, Seo MJ, Kang BW, Park JU, Kim G, Choi BT, Choi YH, Choi YH, et al: Apoptosis induction of human prostate carcinoma cells by cordycepin through reactive oxygen species‑mediated mitochondrial death pathway. Int J Oncol 42: 1036-1044, 2013.
APA
Lee, H.H., Park, C., Jeong, J., Kim, M.J., Seo, M.J., Kang, B.W. ... Jeong, Y.K. (2013). Apoptosis induction of human prostate carcinoma cells by cordycepin through reactive oxygen species‑mediated mitochondrial death pathway. International Journal of Oncology, 42, 1036-1044. https://doi.org/10.3892/ijo.2013.1762
MLA
Lee, H. H., Park, C., Jeong, J., Kim, M. J., Seo, M. J., Kang, B. W., Park, J. U., Kim, G., Choi, B. T., Choi, Y. H., Jeong, Y. K."Apoptosis induction of human prostate carcinoma cells by cordycepin through reactive oxygen species‑mediated mitochondrial death pathway". International Journal of Oncology 42.3 (2013): 1036-1044.
Chicago
Lee, H. H., Park, C., Jeong, J., Kim, M. J., Seo, M. J., Kang, B. W., Park, J. U., Kim, G., Choi, B. T., Choi, Y. H., Jeong, Y. K."Apoptosis induction of human prostate carcinoma cells by cordycepin through reactive oxygen species‑mediated mitochondrial death pathway". International Journal of Oncology 42, no. 3 (2013): 1036-1044. https://doi.org/10.3892/ijo.2013.1762
Copy and paste a formatted citation
x
Spandidos Publications style
Lee HH, Park C, Jeong J, Kim MJ, Seo MJ, Kang BW, Park JU, Kim G, Choi BT, Choi YH, Choi YH, et al: Apoptosis induction of human prostate carcinoma cells by cordycepin through reactive oxygen species‑mediated mitochondrial death pathway. Int J Oncol 42: 1036-1044, 2013.
APA
Lee, H.H., Park, C., Jeong, J., Kim, M.J., Seo, M.J., Kang, B.W. ... Jeong, Y.K. (2013). Apoptosis induction of human prostate carcinoma cells by cordycepin through reactive oxygen species‑mediated mitochondrial death pathway. International Journal of Oncology, 42, 1036-1044. https://doi.org/10.3892/ijo.2013.1762
MLA
Lee, H. H., Park, C., Jeong, J., Kim, M. J., Seo, M. J., Kang, B. W., Park, J. U., Kim, G., Choi, B. T., Choi, Y. H., Jeong, Y. K."Apoptosis induction of human prostate carcinoma cells by cordycepin through reactive oxygen species‑mediated mitochondrial death pathway". International Journal of Oncology 42.3 (2013): 1036-1044.
Chicago
Lee, H. H., Park, C., Jeong, J., Kim, M. J., Seo, M. J., Kang, B. W., Park, J. U., Kim, G., Choi, B. T., Choi, Y. H., Jeong, Y. K."Apoptosis induction of human prostate carcinoma cells by cordycepin through reactive oxygen species‑mediated mitochondrial death pathway". International Journal of Oncology 42, no. 3 (2013): 1036-1044. https://doi.org/10.3892/ijo.2013.1762
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