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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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March 2013 Volume 42 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met

  • Authors:
    • Li-Guang Wang
    • Yang Ni
    • Ben-Hua Su
    • Xue-Ru Mu
    • Hong-Chang Shen
    • Jia-Jun Du
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Provincial Hospital Affiliated to Shandong University, Shandong University, Jinan, P.R. China, Department of Medical Engineering, Provincial Hospital Affiliated to Shandong University, Shandong University, Jinan, P.R. China, Institute for Cancer Research, Provincial Hospital Affiliated to Shandong University, Shandong University, Jinan, P.R. China
  • Pages: 957-962
    |
    Published online on: January 10, 2013
       https://doi.org/10.3892/ijo.2013.1767
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Abstract

MicroRNAs (miRNAs), as a class of naturally occurring small non-coding RNAs, play profound and pervasive roles in cancer initiation and progression. Extensive decrease in miRNA levels are frequently observed in human cancers, indicating that miRNAs may function intrinsically in tumor suppression. However, the underlying mechanisms of miRNA interactions with cellular pathways are still unclear. The expression of miR-34b in non-small cell lung cancer (NSCLC) tissues was detected using quantitative real-time PCR. The relations between miR-34b expression levels and pathological stage or lymph node metastasis were assessed using the Spearman correlation test. For in vitro studies, lung cancer cells were transfected with double stranded synthetic miRNA mimics (syn-hsa-miR-34b miScript miRNA) and scrambled controls. Immunohistochemistry was used to validate the related downstream proteins of miR-34b. The expression of miR-34b was lower in NSCLC tissues compared to that in pericarcinous tissues of lung cancer. Additionally, the Spearman correlation test showed that lower miR-34b expression was correlated with higher lymph node metastasis. In vitro gain-of-function experiments indicated that miR-34b suppressed cell proliferation by inducing cell apoptosis. IHC results showed association between lower miR-34b and overexpression of phospho-Met, p53 (phospho S392) and Mdm2. Consistent with the opposing correlation between the expression of miR-34b and lymph node metastasis in NSCLC, miR-34b may play an important role in NSCLC progression. Furthermore, miR-34b downregulates Met, with subsequent changes of downstream p53 (phospho S392) and Mdm2, and inversely p53 upregulates miR-34b in a feedback loop, which provides new insights into the roles of miR-34 family members in the regulation of signaling pathways of NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Ni Y, Su B, Mu X, Shen H and Du J: MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met. Int J Oncol 42: 957-962, 2013.
APA
Wang, L., Ni, Y., Su, B., Mu, X., Shen, H., & Du, J. (2013). MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met. International Journal of Oncology, 42, 957-962. https://doi.org/10.3892/ijo.2013.1767
MLA
Wang, L., Ni, Y., Su, B., Mu, X., Shen, H., Du, J."MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met". International Journal of Oncology 42.3 (2013): 957-962.
Chicago
Wang, L., Ni, Y., Su, B., Mu, X., Shen, H., Du, J."MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met". International Journal of Oncology 42, no. 3 (2013): 957-962. https://doi.org/10.3892/ijo.2013.1767
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Ni Y, Su B, Mu X, Shen H and Du J: MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met. Int J Oncol 42: 957-962, 2013.
APA
Wang, L., Ni, Y., Su, B., Mu, X., Shen, H., & Du, J. (2013). MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met. International Journal of Oncology, 42, 957-962. https://doi.org/10.3892/ijo.2013.1767
MLA
Wang, L., Ni, Y., Su, B., Mu, X., Shen, H., Du, J."MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met". International Journal of Oncology 42.3 (2013): 957-962.
Chicago
Wang, L., Ni, Y., Su, B., Mu, X., Shen, H., Du, J."MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met". International Journal of Oncology 42, no. 3 (2013): 957-962. https://doi.org/10.3892/ijo.2013.1767
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