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Article

EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells

  • Authors:
    • Jung Sun Park
    • Pham Ngoc Khoi
    • Young Eun Joo
    • Young Han Lee
    • Sven A. Lang
    • Oliver Stoeltzing
    • Young Do Jung
  • View Affiliations / Copyright

    Affiliations: Research Institute of Medical Sciences, Chonnam National University Medical School, Gwangju, Republic of Korea, Department of Biomedical Science and Technology, SMART Institute of Advanced Biomedical Science, RCTC, Konkuk University, Seoul, Republic of Korea, Department of Surgery, University Medical Center Regensburg, Regensburg, Germany, Department of Hepatobiliary and Transplant Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Pages: 1120-1126
    |
    Published online on: January 16, 2013
       https://doi.org/10.3892/ijo.2013.1775
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Abstract

Abnormal accumulation and activation of the recepteur d'origine nantais (RON) has been implicated in epithelial tumor carcinogenesis. In the present study, we examined the effect of epigallocatechin-3-gallate (EGCG), the major green tea catechin, on the induction of RON and tumor growth in human gastric cancer. EGCG inhibited phorbol 12‑myristate 13‑acetate (PMA)‑induced RON expression and reduced RON transcriptional activity. However, (-)‑epigalloca­techin (EGC), (-)‑epicatechin gallate (ECG) and (-)‑epicatechin (EC) did not affect RON expression. Experiments with deleted and site‑directed mutagenesis of the RON promoter indicated that Egr-1 binding sites in the RON promoter may be the EGCG‑response element acting as a cis‑element in gastric cancer cells. EGCG also inhibited PMA-induced Egr-1 expression and DNA binding in a dose-dependent manner. Furthermore, gastric cancer cells pretreated with PMA showed markedly enhanced invasiveness, which was partially abrogated by EGCG and siRNA‑targeted RON and Egr-1. EGCG significantly reduced tumor growth in an in vivo tumor model, whereas RON expression was downregulated. These results suggest that EGCG may exert at least part of its anticancer effect by controlling RON expression through suppression of Egr-1 activation.
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Copy and paste a formatted citation
Spandidos Publications style
Park JS, Khoi PN, Joo YE, Lee YH, Lang SA, Stoeltzing O and Jung YD: EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells. Int J Oncol 42: 1120-1126, 2013.
APA
Park, J.S., Khoi, P.N., Joo, Y.E., Lee, Y.H., Lang, S.A., Stoeltzing, O., & Jung, Y.D. (2013). EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells. International Journal of Oncology, 42, 1120-1126. https://doi.org/10.3892/ijo.2013.1775
MLA
Park, J. S., Khoi, P. N., Joo, Y. E., Lee, Y. H., Lang, S. A., Stoeltzing, O., Jung, Y. D."EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells". International Journal of Oncology 42.3 (2013): 1120-1126.
Chicago
Park, J. S., Khoi, P. N., Joo, Y. E., Lee, Y. H., Lang, S. A., Stoeltzing, O., Jung, Y. D."EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells". International Journal of Oncology 42, no. 3 (2013): 1120-1126. https://doi.org/10.3892/ijo.2013.1775
Copy and paste a formatted citation
x
Spandidos Publications style
Park JS, Khoi PN, Joo YE, Lee YH, Lang SA, Stoeltzing O and Jung YD: EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells. Int J Oncol 42: 1120-1126, 2013.
APA
Park, J.S., Khoi, P.N., Joo, Y.E., Lee, Y.H., Lang, S.A., Stoeltzing, O., & Jung, Y.D. (2013). EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells. International Journal of Oncology, 42, 1120-1126. https://doi.org/10.3892/ijo.2013.1775
MLA
Park, J. S., Khoi, P. N., Joo, Y. E., Lee, Y. H., Lang, S. A., Stoeltzing, O., Jung, Y. D."EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells". International Journal of Oncology 42.3 (2013): 1120-1126.
Chicago
Park, J. S., Khoi, P. N., Joo, Y. E., Lee, Y. H., Lang, S. A., Stoeltzing, O., Jung, Y. D."EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells". International Journal of Oncology 42, no. 3 (2013): 1120-1126. https://doi.org/10.3892/ijo.2013.1775
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