Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells

  • Authors:
    • Guangwen Wu
    • Jianfeng Chu
    • Zhengrong Huang
    • Jinxia Ye
    • Panyu Chen
    • Chunsong Zheng
    • Xihai Li
    • Xianxiang Liu
    • Mingxia Wu
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  • Published online on: January 23, 2013     https://doi.org/10.3892/ijo.2013.1795
  • Pages: 1070-1080
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Abstract

Xiao Jin Wan (XJW) is a well-known traditional Chinese folk-medicine, which is commonly used for the treatment of various types of diseases including cancers. However, the mechanism of the anticancer activity of XJW against U-2OS human osteosarcoma cells, have not yet been reported. In the present study, we investigated the cellular effects of the XJW on the U-2OS human osteosarcoma cell line. Our results showed that XJW induced cell morphological changes, reduced cell viability in a dose- and time-dependent manner and arrested in the G2/M phase of the cell cycle suggesting that XJW inhibited the proliferation of U-2OS cells. Hoechst 33258 staining and Annexin V/propidium iodide double staining exhibited the typical nuclear features of apoptosis and increased the proportion of apoptotic Annexin V-positive cells in a dose-dependent manner, respectively. In addition, XJW treatment caused loss of plasma membrane asymmetry, collapse of mitochondrial membrane potential, activation of caspase-9 and caspase-3, and increase of the ratio of pro-apoptotic Bax to anti-apoptotic Bcl-2. Taken together, the results indicate that the U-2OS cell growth inhibitory activity of XJW was due to cell cycle arrested and mitochondrial-mediated apoptosis, which may partly explain the anticancer activity of Xiao Jin Wan.
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March 2013
Volume 42 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wu G, Chu J, Huang Z, Ye J, Chen P, Zheng C, Li X, Liu X and Wu M: Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells. Int J Oncol 42: 1070-1080, 2013
APA
Wu, G., Chu, J., Huang, Z., Ye, J., Chen, P., Zheng, C. ... Wu, M. (2013). Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells. International Journal of Oncology, 42, 1070-1080. https://doi.org/10.3892/ijo.2013.1795
MLA
Wu, G., Chu, J., Huang, Z., Ye, J., Chen, P., Zheng, C., Li, X., Liu, X., Wu, M."Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells". International Journal of Oncology 42.3 (2013): 1070-1080.
Chicago
Wu, G., Chu, J., Huang, Z., Ye, J., Chen, P., Zheng, C., Li, X., Liu, X., Wu, M."Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells". International Journal of Oncology 42, no. 3 (2013): 1070-1080. https://doi.org/10.3892/ijo.2013.1795