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International Journal of Oncology
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Article

Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells

  • Authors:
    • Guangwen Wu
    • Jianfeng Chu
    • Zhengrong Huang
    • Jinxia Ye
    • Panyu Chen
    • Chunsong Zheng
    • Xihai Li
    • Xianxiang Liu
    • Mingxia Wu
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China, Department of Integrating Τraditional Chinese and Western Medicine, Fujian Provincial Cancer Hospital, Fuzhou 350014, P.R. China, Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China, Second Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, P.R. China
  • Pages: 1070-1080
    |
    Published online on: January 23, 2013
       https://doi.org/10.3892/ijo.2013.1795
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Abstract

Xiao Jin Wan (XJW) is a well-known traditional Chinese folk-medicine, which is commonly used for the treatment of various types of diseases including cancers. However, the mechanism of the anticancer activity of XJW against U-2OS human osteosarcoma cells, have not yet been reported. In the present study, we investigated the cellular effects of the XJW on the U-2OS human osteosarcoma cell line. Our results showed that XJW induced cell morphological changes, reduced cell viability in a dose- and time-dependent manner and arrested in the G2/M phase of the cell cycle suggesting that XJW inhibited the proliferation of U-2OS cells. Hoechst 33258 staining and Annexin V/propidium iodide double staining exhibited the typical nuclear features of apoptosis and increased the proportion of apoptotic Annexin V-positive cells in a dose-dependent manner, respectively. In addition, XJW treatment caused loss of plasma membrane asymmetry, collapse of mitochondrial membrane potential, activation of caspase-9 and caspase-3, and increase of the ratio of pro-apoptotic Bax to anti-apoptotic Bcl-2. Taken together, the results indicate that the U-2OS cell growth inhibitory activity of XJW was due to cell cycle arrested and mitochondrial-mediated apoptosis, which may partly explain the anticancer activity of Xiao Jin Wan.
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Copy and paste a formatted citation
Spandidos Publications style
Wu G, Chu J, Huang Z, Ye J, Chen P, Zheng C, Li X, Liu X and Wu M: Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells. Int J Oncol 42: 1070-1080, 2013.
APA
Wu, G., Chu, J., Huang, Z., Ye, J., Chen, P., Zheng, C. ... Wu, M. (2013). Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells. International Journal of Oncology, 42, 1070-1080. https://doi.org/10.3892/ijo.2013.1795
MLA
Wu, G., Chu, J., Huang, Z., Ye, J., Chen, P., Zheng, C., Li, X., Liu, X., Wu, M."Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells". International Journal of Oncology 42.3 (2013): 1070-1080.
Chicago
Wu, G., Chu, J., Huang, Z., Ye, J., Chen, P., Zheng, C., Li, X., Liu, X., Wu, M."Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells". International Journal of Oncology 42, no. 3 (2013): 1070-1080. https://doi.org/10.3892/ijo.2013.1795
Copy and paste a formatted citation
x
Spandidos Publications style
Wu G, Chu J, Huang Z, Ye J, Chen P, Zheng C, Li X, Liu X and Wu M: Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells. Int J Oncol 42: 1070-1080, 2013.
APA
Wu, G., Chu, J., Huang, Z., Ye, J., Chen, P., Zheng, C. ... Wu, M. (2013). Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells. International Journal of Oncology, 42, 1070-1080. https://doi.org/10.3892/ijo.2013.1795
MLA
Wu, G., Chu, J., Huang, Z., Ye, J., Chen, P., Zheng, C., Li, X., Liu, X., Wu, M."Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells". International Journal of Oncology 42.3 (2013): 1070-1080.
Chicago
Wu, G., Chu, J., Huang, Z., Ye, J., Chen, P., Zheng, C., Li, X., Liu, X., Wu, M."Xiao Jin Wan, a traditional Chinese herbal formula, inhibits proliferation via arresting cell cycle progression at the G2/M phase and promoting apoptosis via activating the mitochondrial‑dependent pathway in U-2OS human osteosarcoma cells". International Journal of Oncology 42, no. 3 (2013): 1070-1080. https://doi.org/10.3892/ijo.2013.1795
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