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International Journal of Oncology
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Article

Candidate of metastasis 1 regulates in vitro growth and invasion of bladder cancer cells

Corrigendum in: /10.3892/ijo.2018.4277
  • Authors:
    • Peng Du
    • Lin Ye
    • Yong Yang
    • Wen G. Jiang
  • View Affiliations / Copyright

    Affiliations: Cardiff University-Peking University School of Oncology Joint Institute, Cardiff CF14 4XN, UK, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Urology, Peking University Cancer Hospital, Haidian District, Beijing, P.R. China
  • Pages: 1249-1256
    |
    Published online on: January 29, 2013
       https://doi.org/10.3892/ijo.2013.1802
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Abstract

COM1 (candidate of metastasis 1) has been recently shown to influence the metastatic ability of cancer cells and disease progression of certain solid tumours. The role of COM1 in bladder cancer remains unknown. In the present study, we examined the expression of the COM1 protein in human bladder tissues, and also its effect on growth, adhesion, migration and invasion of human bladder cancer cells, in vitro. The expression of COM1 in human bladder tissues and bladder cancer cell lines was assessed at both the mRNA and protein levels using RT-PCR and immunohistochemistry, respectively. COM1 staining was compared with tumour staging. Mammalian COM1 expression construct and anti-COM1 ribozyme transgenes were used to generate sublines of human bladder cancer cells with differential expression of COM1. The effect of COM1 on cellular functions was examined in bladder cancer cells with which COM1 was overexpressed or knocked down using a variety of in vitro assays. In normal bladder tissues, stronger staining of COM1 was seen in the cytoplasm of normal urothelial cells. In contrast, the staining was notably weak or absent in cancer cells of tumour tissues and invasive tumours had significantly low levels of staining compared with non-invasive tumours (p=0.012). Knockdown of COM1 in bladder cancer cell lines resulted in an increase in cellular growth and invasion, while overexpression of COM1 suppressed invasiveness and growth of these cells. Further investigation revealed an increased apoptosis and upregulated p21 in bladder cancer cells when COM1 was overexpressed. COM1 is expressed at low levels in human bladder cancer and in particular in invasive bladder tumours. COM1 levels are inversely correlated with the invasiveness and growth of bladder cancer cells in vitro. Induced apoptosis and upregulation of p21 are indicated in the mechanism of COM1 inhibiting bladder cancer cell growth. It suggests that COM1 is a potential tumour suppressor in human bladder cancer.
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1 

Ree AH, Tvermyr M, Engebraaten O, Rooman M, Rosok O, Hovig E, Meza-Zepeda LA, Bruland OS and Fodstad O: Expression of a novel factor in human breast cancer cells with metastatic potential. Cancer Res. 59:4675–4680. 1999.

2 

Mallo GV, Fiedler F, Calvo EL, Ortiz EM, Vasseur S, Keim V, Morisset J and Iovanna JL: Cloning and expression of the rat p8 cDNA, a new gene activated in pancreas during the acute phase of pancreatitis, pancreatic development, and regeneration, and which promotes cellular growth. J Biol Chem. 272:32360–32369. 1997. View Article : Google Scholar

3 

Su SB, Motoo Y, Iovanna JL, Berthezene P, Xie MJ, Mouri H, Ohtsubo K, Matsubara F and Sawabu N: Overexpression of p8 is inversely correlated with apoptosis in pancreatic cancer. Clin Cancer Res. 7:1320–1324. 2001.PubMed/NCBI

4 

Ito Y, Yoshida H, Motoo Y, et al: Expression and cellular localization of p8 protein in thyroid neoplasms. Cancer Lett. 201:237–244. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Jiang WG, Watkins G, Douglas-Jones A, Mokbel K, Mansel RE and Fodstad O: Expression of COM-1/P8 in human breast cancer and its relevance to clinical outcome and ER status. Int J Cancer. 117:730–737. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Jiang WG, Davies G and Fodstad O: COM-1/P8 in oestrogen regulated growth of breast cancer cells, the ER-beta connection. Biochem Biophys Res Commun. 330:253–262. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Jiang WG, Davies G, Martin TA, Kynaston H, Mason MD and Fodstad O: COM-1/p8 acts as a putative tumour suppressor in prostate cancer. Int J Mol Med. 18:981–986. 2006.PubMed/NCBI

8 

Clark DW, Mitra A, Fillmore RA, Jiang WG, Samant RS, Fodstad O and Shevde LA: NUPR1 interacts with p53, transcriptionally regulates p21 and rescues breast epithelial cells from doxorubicin-induced genotoxic stress. Curr Cancer Drug Targets. 8:421–430. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Allred DC, Harvey JM, Berardo M and Clark GM: Prognostic and predictive factors in breast cancer by immunohistochemical analysis. Mod Pathol. 11:155–168. 1998.PubMed/NCBI

10 

Yu H, Ye L, Mansel RE, Zhang Y and Jiang WG: Clinical implications of the influence of Ehm2 on the aggressiveness of breast cancer cells through regulation of matrix metalloproteinase-9 expression. Mol Cancer Res. 8:1501–1512. 2010. View Article : Google Scholar

11 

Ploeg M, Aben KK and Kiemeney LA: The present and future burden of urinary bladder cancer in the world. World J Urol. 27:289–293. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Youssef RF and Lotan Y: Predictors of outcome of non-muscle-invasive and muscle-invasive bladder cancer. Scientific World J. 11:369–381. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Ree AH, Pacheco MM, Tvermyr M, Fodstad O and Brentani MM: Expression of a novel factor, com1, in early tumor progression of breast cancer. Clin Cancer Res. 6:1778–1783. 2000.PubMed/NCBI

14 

Ito Y, Yoshida H, Motoo Y, et al: Expression of p8 protein in medullary thyroid carcinoma. Anticancer Res. 25:3419–3423. 2005.PubMed/NCBI

15 

Ree AH, Bratland A, Kroes RA, Aasheim HC, Florenes VA, Moskal JR, Fodstad O, Bruland OS and Maelandsmo GM: Clinical and cell line specific expression profiles of a human gene identified in experimental central nervous system metastases. Anticancer Res. 22:1949–1957. 2002.PubMed/NCBI

16 

Giroux V, Malicet C, Barthet M, Gironella M, Archange C, Dagorn JC, Vasseur S and Iovanna JL: p8 is a new target of gemcitabine in pancreatic cancer cells. Clin Cancer Res. 12:235–241. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Su SB, Motoo Y, Iovanna JL, et al: Expression of p8 in human pancreatic cancer. Clin Cancer Res. 7:309–313. 2001.PubMed/NCBI

18 

Bratland A, Risberg K, Maelandsmo GM, et al: Expression of a novel factor, com1, is regulated by 1,25-dihydroxyvitamin D3 in breast cancer cells. Cancer Res. 60:5578–5583. 2000.PubMed/NCBI

19 

Trump DL, Muindi J, Fakih M, Yu WD and Johnson CS: Vitamin D compounds: clinical development as cancer therapy and prevention agents. Anticancer Res. 26:2551–2556. 2006.PubMed/NCBI

20 

Niculescu AB III, Chen X, Smeets M, Hengst L, Prives C and Reed SI: Effects of p21(Cip1/Waf1) at both the G1/S and the G2/M cell cycle transitions: pRb is a critical determinant in blocking DNA replication and in preventing endoreduplication. Mol Cell Biol. 18:629–643. 1998.

21 

Smits VA and Medema RH: Checking out the G(2)/M transition. Biochim Biophys Acta. 1519:1–12. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Malicet C, Giroux V, Vasseur S, Dagorn JC, Neira JL and Iovanna JL: Regulation of apoptosis by the p8/prothymosin alpha complex. Proc Natl Acad Sci USA. 103:2671–2676. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Jiang WG, Davies G, Kynaston H, Mason MD and Fodstad O: Does the PGC-1/PPARgamma pathway play a role in COM-1/p8 mediated cell growth inhibition in prostate cancer? Int J Mol Med. 18:1169–1175. 2006.PubMed/NCBI

24 

Goruppi S and Ionanna JL: Stress-inducible protein p8 is involved in several physiological and pathological processes. J Biol Chem. 285:1577–1581. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Davies ML, Parr C, Sanders AJ, Fodstad O and Jiang WG: The transcript expression and protein distribution pattern in human colorectal carcinoma reveal a pivotal role of COM-1/p8 as a tumour suppressor. Cancer Genomics Proteomics. 7:75–80. 2010.

26 

Li X, Martin TA and Jiang WG: COM-1/p8 acts as a tumour growth enhancer in colorectal cancer cell lines. Anticancer Res. 32:1229–1237. 2012.PubMed/NCBI

27 

Frisch SM and Francis HL: Disruption of epithelial cell-matrix interactions induces apoptosis. J Cell Biol. 124:619–626. 1994. View Article : Google Scholar : PubMed/NCBI

28 

Shao J, Sheng H, DuBois RN and Beauchamp RD: Oncogeneic Ras-medicated cell growth arrest and apoptosis are associated with increased ubiquitin-dependent cyclin D1 degradation. J Biol Chem. 275:22916–22924. 2000. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Du P, Ye L, Yang Y and Jiang WG: Candidate of metastasis 1 regulates in vitro growth and invasion of bladder cancer cells Corrigendum in /10.3892/ijo.2018.4277. Int J Oncol 42: 1249-1256, 2013.
APA
Du, P., Ye, L., Yang, Y., & Jiang, W.G. (2013). Candidate of metastasis 1 regulates in vitro growth and invasion of bladder cancer cells Corrigendum in /10.3892/ijo.2018.4277. International Journal of Oncology, 42, 1249-1256. https://doi.org/10.3892/ijo.2013.1802
MLA
Du, P., Ye, L., Yang, Y., Jiang, W. G."Candidate of metastasis 1 regulates in vitro growth and invasion of bladder cancer cells Corrigendum in /10.3892/ijo.2018.4277". International Journal of Oncology 42.4 (2013): 1249-1256.
Chicago
Du, P., Ye, L., Yang, Y., Jiang, W. G."Candidate of metastasis 1 regulates in vitro growth and invasion of bladder cancer cells Corrigendum in /10.3892/ijo.2018.4277". International Journal of Oncology 42, no. 4 (2013): 1249-1256. https://doi.org/10.3892/ijo.2013.1802
Copy and paste a formatted citation
x
Spandidos Publications style
Du P, Ye L, Yang Y and Jiang WG: Candidate of metastasis 1 regulates in vitro growth and invasion of bladder cancer cells Corrigendum in /10.3892/ijo.2018.4277. Int J Oncol 42: 1249-1256, 2013.
APA
Du, P., Ye, L., Yang, Y., & Jiang, W.G. (2013). Candidate of metastasis 1 regulates in vitro growth and invasion of bladder cancer cells Corrigendum in /10.3892/ijo.2018.4277. International Journal of Oncology, 42, 1249-1256. https://doi.org/10.3892/ijo.2013.1802
MLA
Du, P., Ye, L., Yang, Y., Jiang, W. G."Candidate of metastasis 1 regulates in vitro growth and invasion of bladder cancer cells Corrigendum in /10.3892/ijo.2018.4277". International Journal of Oncology 42.4 (2013): 1249-1256.
Chicago
Du, P., Ye, L., Yang, Y., Jiang, W. G."Candidate of metastasis 1 regulates in vitro growth and invasion of bladder cancer cells Corrigendum in /10.3892/ijo.2018.4277". International Journal of Oncology 42, no. 4 (2013): 1249-1256. https://doi.org/10.3892/ijo.2013.1802
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