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International Journal of Oncology
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Article

Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells

  • Authors:
    • Masashi Shiiba
    • Kengo Saito
    • Kazuaki Fushimi
    • Takashi Ishigami
    • Keiji Shinozuka
    • Dai Nakashima
    • Yukinao Kouzu
    • Hirofumi Koike
    • Atsushi Kasamatsu
    • Yosuke Sakamoto
    • Katsunori Ogawara
    • Katsuhiro Uzawa
    • Yuichi Takiguchi
    • Hideki Tanzawa
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba 260-8670, Japan, Department of Molecular Virology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba 260-8670, Japan, Department of Medical Oncology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba 260-8670, Japan
  • Pages: 1197-1204
    |
    Published online on: February 8, 2013
       https://doi.org/10.3892/ijo.2013.1815
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Abstract

The aim of the present study was to identify a target molecule that could predict the efficacy of radiotherapy in oral squamous cell carcinoma (OSCC). We used DNA microarray analysis to identify differences in gene expression after X-ray irradiation. We compared the gene expression profiles between X-ray (8 Gy)-irradiated Ca9-22 cells (an OSCC-derived cell line) and unirradiated Ca9-22 cells. A total of 167 genes with a 2-fold higher level of expression induced by X-ray irradiation were identified. Lipocalin-2 (LCN2) had the greatest increase in expression after X-ray irradiation, and it was categorized in a network that has cancer-related functions with the Ingenuity Pathway Analysis tool. Upregulated expression of LCN2 mRNA was validated by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis. When the LCN2 gene was knocked down in OSCC cells (Ca9-22 and HSC-2) and lung cancer cells (A549) by using small interfering RNA, the radiosensitivity of these cells was enhanced. Our findings suggest that the overexpression of LCN2 is likely associated with radioresistance in oral cancer and lung cancer cells, and that LCN2 expression levels could be used to predict radioresistance. Thus, regulating the expression or function of LCN2 could enhance the radiation response, resulting in a favorable outcome of radiotherapy.
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1 

Lehnert S: Prediction of tumor response to therapy: molecular markers and the microenvironment. Apoptosis and chips: an overview of the proceedings. Radiat Res. 154:121–124. 2000.PubMed/NCBI

2 

Joki T, Carroll RS, Dunn IF, Zhang J, Abe T and Black PM: Assessment of alterations in gene expression in recurrent malignant glioma after radiotherapy using complementary deoxyribonucleic acid microarrays. Neurosurgery. 48:195–202. 2001.PubMed/NCBI

3 

Keyse SM: The induction of gene expression in mammalian cells by radiation. Semin Cancer Biol. 4:119–128. 1993.PubMed/NCBI

4 

Iliakis G: Cell cycle regulation in irradiated and nonirradiated cells. Semin Oncol. 24:602–615. 1997.PubMed/NCBI

5 

Eckardt-Schupp F and Klaus C: Radiation inducible DNA repair processes in eukaryotes. Biochimie. 81:161–171. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Maity A, McKenna WG and Muschel RJ: The molecular basis for cell cycle delays following ionizing radiation: a review. Radiother Oncol. 31:1–13. 1994. View Article : Google Scholar : PubMed/NCBI

7 

Forrester HB, Vidair CA, Albright N, Ling CC and Dewey WC: Using computerized video time lapse for quantifying cell death of X-irradiated rat embryo cells transfected with c-myc or c-Ha-ras. Cancer Res. 59:931–939. 1999.PubMed/NCBI

8 

Terakado N, Shintani S, Yano J, et al: Overexpression of cyclooxygenase-2 is associated with radioresistance in oral squamous cell carcinoma. Oral Oncol. 40:383–389. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Freier K, Pungs S, Sticht C, et al: High survivin expression is associated with favorable outcome in advanced primary oral squamous cell carcinoma after radiation therapy. Int J Cancer. 120:942–946. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Yamazaki Y, Chiba I, Hirai A, et al: Radioresistance in oral squamous cell carcinoma with p53 DNA contact mutation. Am J Clin Oncol. 26:e124–e129. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Moergel M, Abt E, Stockinger M and Kunkel M: Overexpression of p63 is associated with radiation resistance and prognosis in oral squamous cell carcinoma. Oral Oncol. 46:667–671. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Gu Y, Fan S, Liu B, et al: TCRP1 promotes radioresistance of oral squamous cell carcinoma cells via Akt signal pathway. Mol Cell Biochem. 357:107–113. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Ishigami T, Uzawa K, Fushimi K, et al: Inhibition of ICAM2 induces radiosensitization in oral squamous cell carcinoma cells. Br J Cancer. 98:1357–1365. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Tong Z, Wu X, Ovcharenko D, Zhu J, Chen CS and Kehrer JP: Neutrophil gelatinase-associated lipocalin as a survival factor. Biochem J. 391:441–448. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Flower DR: The lipocalin protein family: structure and function. Biochem J. 318:1–14. 1996.

16 

Triebel S, Blaser J, Reinke H and Tschesche H: A 25 kDa alpha 2-microglobulin-related protein is a component of the 125 kDa form of human gelatinase. FEBS Lett. 314:386–388. 1992. View Article : Google Scholar : PubMed/NCBI

17 

Nilsen-Hamilton M, Liu Q, Ryon J, Bendickson L, Lepont P and Chang Q: Tissue involution and the acute phase response. Ann NY Acad Sci. 995:94–108. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M and Kjeldsen L: Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases. Gut. 38:414–420. 1996. View Article : Google Scholar : PubMed/NCBI

19 

Bratt T: Lipocalins and cancer. Biochim Biophys Acta. 1482:318–326. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Furutani M, Arii S, Mizumoto M, Kato M and Imamura M: Identification of a neutrophil gelatinase-associated lipocalin mRNA in human pancreatic cancers using a modified signal sequence trap method. Cancer Lett. 122:209–214. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Bartsch S and Tschesche H: Cloning and expression of human neutrophil lipocalin cDNA derived from bone marrow and ovarian cancer cells. FEBS Lett. 357:255–259. 1995. View Article : Google Scholar : PubMed/NCBI

22 

Hiromoto T, Noguchi K, Yamamura M, et al: Upregulation of neutrophil gelatinase-associated lipocalin in oral squamous cell carcinoma: relation to cell differentiation. Oncol Rep. 26:1415–1421. 2011.PubMed/NCBI

23 

Ishigami T, Uzawa K, Higo M, et al: Genes and molecular pathways related to radioresistance of oral squamous cell carcinoma cells. Int J Cancer. 120:2262–2270. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Ogawa K, Utsunomiya T, Mimori K, et al: Differential gene expression profiles of radioresistant pancreatic cancer cell lines established by fractionated irradiation. Int J Oncol. 28:705–713. 2006.PubMed/NCBI

25 

Guo WF, Lin RX, Huang J, et al: Identification of differentially expressed genes contributing to radioresistance in lung cancer cells using microarray analysis. Radiat Res. 164:27–35. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Hellman B, Brodin D, Andersson M, et al: Radiation-induced DNA-damage and gene expression profiles in human lung cancer cells with different radiosensitivity. Exp Oncol. 27:102–107. 2005.PubMed/NCBI

27 

Fukuda K, Sakakura C, Miyagawa K, et al: Differential gene expression profiles of radioresistant oesophageal cancer cell lines established by continuous fractionated irradiation. Br J Cancer. 91:1543–1550. 2004. View Article : Google Scholar

28 

Kitahara O, Katagiri T, Tsunoda T, Harima Y and Nakamura Y: Classification of sensitivity or resistance of cervical cancers to ionizing radiation according to expression profiles of 62 genes selected by cDNA microarray analysis. Neoplasia. 4:295–303. 2002. View Article : Google Scholar

29 

Achary MP, Jaggernauth W, Gross E, Alfieri A, Klinger HP and Vikram B: Cell lines from the same cervical carcinoma but with different radiosensitivities exhibit different cDNA microarray patterns of gene expression. Cytogenet Cell Genet. 91:39–43. 2000. View Article : Google Scholar

30 

Chu ST, Lin HJ, Huang HL and Chen YH: The hydrophobic pocket of 24p3 protein from mouse uterine luminal fluid: fatty acid and retinol binding activity and predicted structural similarity to lipocalins. J Pept Res. 52:390–397. 1998.

31 

Yang J, Goetz D, Li JY, et al: An iron delivery pathway mediated by a lipocalin. Mol Cell. 10:1045–1056. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Cowland JB and Borregaard N: Molecular characterization and pattern of tissue expression of the gene for neutrophil gelatinase-associated lipocalin from humans. Genomics. 45:17–23. 1997. View Article : Google Scholar

33 

Roudkenar MH, Kuwahara Y, Baba T, et al: Oxidative stress induced lipocalin 2 gene expression: addressing its expression under the harmful conditions. J Radiat Res. 48:39–44. 2007. View Article : Google Scholar : PubMed/NCBI

34 

Park S, Guo J, Kim D and Cheng JQ: Identification of 24p3 as a direct target of Foxo3a regulated by interleukin-3 through the phosphoinositide 3-kinase/Akt pathway. J Biol Chem. 284:2187–2193. 2009. View Article : Google Scholar : PubMed/NCBI

35 

Rosen JM and Jordan CT: The increasing complexity of the cancer stem cell paradigm. Science. 324:1670–1673. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shiiba M, Saito K, Fushimi K, Ishigami T, Shinozuka K, Nakashima D, Kouzu Y, Koike H, Kasamatsu A, Sakamoto Y, Sakamoto Y, et al: Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells. Int J Oncol 42: 1197-1204, 2013.
APA
Shiiba, M., Saito, K., Fushimi, K., Ishigami, T., Shinozuka, K., Nakashima, D. ... Tanzawa, H. (2013). Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells. International Journal of Oncology, 42, 1197-1204. https://doi.org/10.3892/ijo.2013.1815
MLA
Shiiba, M., Saito, K., Fushimi, K., Ishigami, T., Shinozuka, K., Nakashima, D., Kouzu, Y., Koike, H., Kasamatsu, A., Sakamoto, Y., Ogawara, K., Uzawa, K., Takiguchi, Y., Tanzawa, H."Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells". International Journal of Oncology 42.4 (2013): 1197-1204.
Chicago
Shiiba, M., Saito, K., Fushimi, K., Ishigami, T., Shinozuka, K., Nakashima, D., Kouzu, Y., Koike, H., Kasamatsu, A., Sakamoto, Y., Ogawara, K., Uzawa, K., Takiguchi, Y., Tanzawa, H."Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells". International Journal of Oncology 42, no. 4 (2013): 1197-1204. https://doi.org/10.3892/ijo.2013.1815
Copy and paste a formatted citation
x
Spandidos Publications style
Shiiba M, Saito K, Fushimi K, Ishigami T, Shinozuka K, Nakashima D, Kouzu Y, Koike H, Kasamatsu A, Sakamoto Y, Sakamoto Y, et al: Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells. Int J Oncol 42: 1197-1204, 2013.
APA
Shiiba, M., Saito, K., Fushimi, K., Ishigami, T., Shinozuka, K., Nakashima, D. ... Tanzawa, H. (2013). Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells. International Journal of Oncology, 42, 1197-1204. https://doi.org/10.3892/ijo.2013.1815
MLA
Shiiba, M., Saito, K., Fushimi, K., Ishigami, T., Shinozuka, K., Nakashima, D., Kouzu, Y., Koike, H., Kasamatsu, A., Sakamoto, Y., Ogawara, K., Uzawa, K., Takiguchi, Y., Tanzawa, H."Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells". International Journal of Oncology 42.4 (2013): 1197-1204.
Chicago
Shiiba, M., Saito, K., Fushimi, K., Ishigami, T., Shinozuka, K., Nakashima, D., Kouzu, Y., Koike, H., Kasamatsu, A., Sakamoto, Y., Ogawara, K., Uzawa, K., Takiguchi, Y., Tanzawa, H."Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells". International Journal of Oncology 42, no. 4 (2013): 1197-1204. https://doi.org/10.3892/ijo.2013.1815
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