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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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May 2013 Volume 42 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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International Journal of Functional Nutrition

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Article

PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells

  • Authors:
    • Zhi-Gang Bai
    • Ying-Jiang Ye
    • Dan-Hua Shen
    • You-Yong Lu
    • Zhong-Tao Zhang
    • Shan Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, P.R. China, Department of Gastroenterological Surgery, Surgical Oncology Laboratory, Peking University People's Hospital, Beijing, P.R. China, Department of Pathology, Peking University People's Hospital, Beijing, P.R. China, Laboratory of Molecular Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University School of Oncology, Beijing Cancer Hospital/Institute, Beijing, P.R. China
  • Pages: 1682-1691
    |
    Published online on: March 28, 2013
       https://doi.org/10.3892/ijo.2013.1875
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Abstract

The prognosis of gastric cancer (GC) is associated with Cdx2 and nuclear PTEN coexpression. This study aimed to determine the expression patterns of Cdx2 and PTEN in various GC tissues and cell lines to identify their relationship in GC. Immunohistochemistry was undertaken to assess the expression patterns of Cdx2 and PTEN in paraffin-embedded specimens of 228 GC patients who had undergone radical D2 gastrostomy with long-term follow-up. Cell growth and tumorigenicity were analyzed in the BGC823 cells with exogenous Cdx2 and any changes in the associated signaling pathways were interpreted in exogenous cdx2 expression and cdx2 knockdown. Cdx2 was found in the nuclei of GC cells in 43.4% (99/228) of the paraffin-embedded biopsies. A higher expression of nuclear PTEN was observed in 36.4% (83/228). Coexpression of Cdx2 and nuclear PTEN was detected in GC tumors (59/228, 25.9%) which correlated with the prognosis of advanced GC patients (p<0.001). The expression levels of Cdx2 and PTEN were variable in the different GC cell lines. However, the trends were similar between PTEN and Cdx2 in GC tissues and cell lines. High expression of Cdx2 and PTEN significantly reduced tumorigenicity in BGC823 cells compared with the empty vector control. Exogenous expression of Cdx2 triggered the upregulation of PTEN expression and decreased PI3K and pAkt expression and vice versa. The coexpression levels of PTEN and Cdx2 in GC tumors correlated with prognosis in GC patients. Cdx2 may play a role in the upregulation of PTEN by triggering PI3K/Akt inactivation in GC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Bai Z, Ye Y, Shen D, Lu Y, Zhang Z and Wang S: PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells. Int J Oncol 42: 1682-1691, 2013.
APA
Bai, Z., Ye, Y., Shen, D., Lu, Y., Zhang, Z., & Wang, S. (2013). PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells. International Journal of Oncology, 42, 1682-1691. https://doi.org/10.3892/ijo.2013.1875
MLA
Bai, Z., Ye, Y., Shen, D., Lu, Y., Zhang, Z., Wang, S."PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells". International Journal of Oncology 42.5 (2013): 1682-1691.
Chicago
Bai, Z., Ye, Y., Shen, D., Lu, Y., Zhang, Z., Wang, S."PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells". International Journal of Oncology 42, no. 5 (2013): 1682-1691. https://doi.org/10.3892/ijo.2013.1875
Copy and paste a formatted citation
x
Spandidos Publications style
Bai Z, Ye Y, Shen D, Lu Y, Zhang Z and Wang S: PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells. Int J Oncol 42: 1682-1691, 2013.
APA
Bai, Z., Ye, Y., Shen, D., Lu, Y., Zhang, Z., & Wang, S. (2013). PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells. International Journal of Oncology, 42, 1682-1691. https://doi.org/10.3892/ijo.2013.1875
MLA
Bai, Z., Ye, Y., Shen, D., Lu, Y., Zhang, Z., Wang, S."PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells". International Journal of Oncology 42.5 (2013): 1682-1691.
Chicago
Bai, Z., Ye, Y., Shen, D., Lu, Y., Zhang, Z., Wang, S."PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells". International Journal of Oncology 42, no. 5 (2013): 1682-1691. https://doi.org/10.3892/ijo.2013.1875
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