Open Access

The miR-221/222 cluster, miR-10b and miR-92a are highly upregulated in metastatic minimally invasive follicular thyroid carcinoma

  • Authors:
    • Tomoo Jikuzono
    • Masashi Kawamoto
    • Hiroshi Yoshitake
    • Kunio Kikuchi
    • Haruki Akasu
    • Hitoshi Ishikawa
    • Mitsuyoshi Hirokawa
    • Akira Miyauchi
    • Shinichi Tsuchiya
    • Kazuo Shimizu
    • Toshihiro Takizawa
  • View Affiliations

  • Published online on: April 2, 2013     https://doi.org/10.3892/ijo.2013.1879
  • Pages: 1858-1868
  • Copyright: © Jikuzono et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Minimally invasive follicular thyroid carcinoma (MI-FTC) is characterized by limited capsular and/or vascular invasion with good long-term outcomes. However, some cases of MI-FTC show a poor prognosis because of severe distant metastasis (i.e., metastatic MI-FTC). Nonetheless, no method has been established for predicting the prognosis of MI-FTC. This study was conducted to identify novel prognostic factors for metastatic MI-FTC by the use of microRNA (miRNA). Thirty-four patients with MI-FTC were categorized into two groups: the metastatic group, M(+) (n=12) and the non-metastatic group, M(-) (n=22). In the M(+) group, distant metastasis was recognized after the initial operation established the diagnosis of MI-FTC. In the M(-) group, no distant metastasis was recognized postoperatively for ≥10 years. Using laser microdissection followed by quantitative real-time PCR and PCR arrays, we performed a comprehensive expression profiling of 667 miRNAs in formalin-fixed, paraffin-embedded samples from the initial MI-FTC operation. Furthermore, we assessed the potential use of miRNAs as novel biomarkers for the metastatic potential of MI-FTC by logistic regression analysis. Comprehensive quantitative analysis of miRNA expression in MI-FTC samples revealed that the miR-221/222 cluster (i.e., miR-221, miR-222 and miR-222*), miR-10b and miR-92a were significantly upregulated in the M(+) group compared with the M(-) group. Interestingly, the expression levels of these miRNAs were also shown to be upregulated in widely invasive FTC (WI-FTC; n=13) that has distant metastasis and worse prognosis, indicating a close similarity in the miRNA expression between metastatic MI-FTC and WI-FTC. Logistic regression analysis revealed that miR-10b made a significant contribution to prognosis (OR 19.759, 95% CI 1.433-272.355, p=0.026). Our findings suggest that miR-10b is a potential prognostic factor for evaluating the metastatic potential of MI-FTC at an initial operation stage.
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June 2013
Volume 42 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Jikuzono T, Kawamoto M, Yoshitake H, Kikuchi K, Akasu H, Ishikawa H, Hirokawa M, Miyauchi A, Tsuchiya S, Shimizu K, Shimizu K, et al: The miR-221/222 cluster, miR-10b and miR-92a are highly upregulated in metastatic minimally invasive follicular thyroid carcinoma. Int J Oncol 42: 1858-1868, 2013
APA
Jikuzono, T., Kawamoto, M., Yoshitake, H., Kikuchi, K., Akasu, H., Ishikawa, H. ... Takizawa, T. (2013). The miR-221/222 cluster, miR-10b and miR-92a are highly upregulated in metastatic minimally invasive follicular thyroid carcinoma. International Journal of Oncology, 42, 1858-1868. https://doi.org/10.3892/ijo.2013.1879
MLA
Jikuzono, T., Kawamoto, M., Yoshitake, H., Kikuchi, K., Akasu, H., Ishikawa, H., Hirokawa, M., Miyauchi, A., Tsuchiya, S., Shimizu, K., Takizawa, T."The miR-221/222 cluster, miR-10b and miR-92a are highly upregulated in metastatic minimally invasive follicular thyroid carcinoma". International Journal of Oncology 42.6 (2013): 1858-1868.
Chicago
Jikuzono, T., Kawamoto, M., Yoshitake, H., Kikuchi, K., Akasu, H., Ishikawa, H., Hirokawa, M., Miyauchi, A., Tsuchiya, S., Shimizu, K., Takizawa, T."The miR-221/222 cluster, miR-10b and miR-92a are highly upregulated in metastatic minimally invasive follicular thyroid carcinoma". International Journal of Oncology 42, no. 6 (2013): 1858-1868. https://doi.org/10.3892/ijo.2013.1879