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Article

NGX6 expression improves the sensitivity of tamoxifen-resistant MCF-7 cells through modulation of the Smad signaling pathway

  • Authors:
    • Wen-Jing Zhao
    • Ke Wang
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasound, Τhe First Affiliated Hospital of China Medical University, Heping District, Shenyang, Liaoning 110001, P.R. China, Department of Cardiac Surgery, Shengjing Hospital of China Medical University, Heping District, Shenyang, Liaoning 110004, P.R. China
  • Pages: 2060-2068
    |
    Published online on: April 8, 2013
       https://doi.org/10.3892/ijo.2013.1886
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Abstract

The effects of nasopharyngeal carcinoma-associated gene 6 (NGX6) in breast cancer was studied. We demonstrated that the levels of the NGX6 protein and mRNA were lower in patients with tamoxifen-resistant tumors compared to patients with tamoxifen-sensitive tumors. Tamoxifen was able to decrease proliferation, increase apoptosis and induce G1 arrest in NGX6-expressing TRM-7 cells in vitro. In order to detect the mechanism(s) of tamoxifen action, we first obtain the three-dimensional structure of the NGX6 protein by using Protein Homology/analogY Recognition Engine (PHYRE). Prediction of the docking between the NGX6 protein and tamoxifen was performed using SYBYL-X 1.3. Furthermore, we found that tamoxifen activated Smad2/3, and increased the expression of Smad4 in NGX6-expressing TRM-7 cells as evaluated by western blot analysis. Smad2/3-targeted siRNA was used to confirm the mechanism(s) of tamoxifen action in NGX6-expressing cells. These results indicated that NGX6 may increase the sensitivity of breast cancer cells to tamoxifen.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao W and Wang K: NGX6 expression improves the sensitivity of tamoxifen-resistant MCF-7 cells through modulation of the Smad signaling pathway. Int J Oncol 42: 2060-2068, 2013.
APA
Zhao, W., & Wang, K. (2013). NGX6 expression improves the sensitivity of tamoxifen-resistant MCF-7 cells through modulation of the Smad signaling pathway. International Journal of Oncology, 42, 2060-2068. https://doi.org/10.3892/ijo.2013.1886
MLA
Zhao, W., Wang, K."NGX6 expression improves the sensitivity of tamoxifen-resistant MCF-7 cells through modulation of the Smad signaling pathway". International Journal of Oncology 42.6 (2013): 2060-2068.
Chicago
Zhao, W., Wang, K."NGX6 expression improves the sensitivity of tamoxifen-resistant MCF-7 cells through modulation of the Smad signaling pathway". International Journal of Oncology 42, no. 6 (2013): 2060-2068. https://doi.org/10.3892/ijo.2013.1886
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao W and Wang K: NGX6 expression improves the sensitivity of tamoxifen-resistant MCF-7 cells through modulation of the Smad signaling pathway. Int J Oncol 42: 2060-2068, 2013.
APA
Zhao, W., & Wang, K. (2013). NGX6 expression improves the sensitivity of tamoxifen-resistant MCF-7 cells through modulation of the Smad signaling pathway. International Journal of Oncology, 42, 2060-2068. https://doi.org/10.3892/ijo.2013.1886
MLA
Zhao, W., Wang, K."NGX6 expression improves the sensitivity of tamoxifen-resistant MCF-7 cells through modulation of the Smad signaling pathway". International Journal of Oncology 42.6 (2013): 2060-2068.
Chicago
Zhao, W., Wang, K."NGX6 expression improves the sensitivity of tamoxifen-resistant MCF-7 cells through modulation of the Smad signaling pathway". International Journal of Oncology 42, no. 6 (2013): 2060-2068. https://doi.org/10.3892/ijo.2013.1886
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