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International Journal of Oncology
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June 2013 Volume 42 Issue 6

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Article

Proteomic identification of angiomotin by ProteomeLab PF-2D and correlation with clinical outcome in human clear cell renal cell carcinoma

  • Authors:
    • Jin Yang
    • Peijun Liu
    • Meijuan Tian
    • Yuyao Li
    • Wei Chen
    • Xu Li
  • View Affiliations / Copyright

    Affiliations: Medical Oncology Department, The First Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710061, P.R. China, Center for Translational Medicine, The First Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710061, P.R. China
  • Pages: 2078-2086
    |
    Published online on: April 10, 2013
       https://doi.org/10.3892/ijo.2013.1889
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Abstract

Identification of new therapeutic and prognostic biomarkers for clear cell renal cell carcinoma (ccRCC) is urgently required since most patients are in advanced stages of ccRCC at initial diagnosis and the recurrence rate is high. Differentially expressed proteins between the ccRCC cell line RLC-310 and the normal renal cell line HK-2 were identified by a comparative proteomic approach based on a protein fractionation two-dimensional (PF-2D) liquid-phase fractionation system and capillary liquid chromatography electrospray ionization mass spectrometry/mass spectrometry (LC-ESI-MS/MS). Differentially expressed proteins (n=196) were identified. Of the 13 differentially expressed proteins newly discovered in ccRCC, angiomotin (Amot) was the focus of this study since its role in ccRCC progression has been obscure. The overexpression of Amot in ccRCC tissues was confirmed by comparing Amot expression in 18 primary ccRCC tissues and adjacent normal renal tissues (ANRT) using western blot analysis. Quantitative RT-PCR using 127 ccRCC tissues revealed that high levels of Amot transcripts were associated with poor differentiation, venous invasion and decreased survival (p<0.0001, <0.05 and <0.05). Multivariate analysis indicated that Amot transcript was an independent prognostic factor for the survival of ccRCC patients (p<0.05). These data suggest that Amot may serve as a novel prognostic factor of ccRCC.
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Copy and paste a formatted citation
Spandidos Publications style
Yang J, Liu P, Tian M, Li Y, Chen W and Li X: Proteomic identification of angiomotin by ProteomeLab PF-2D and correlation with clinical outcome in human clear cell renal cell carcinoma. Int J Oncol 42: 2078-2086, 2013.
APA
Yang, J., Liu, P., Tian, M., Li, Y., Chen, W., & Li, X. (2013). Proteomic identification of angiomotin by ProteomeLab PF-2D and correlation with clinical outcome in human clear cell renal cell carcinoma. International Journal of Oncology, 42, 2078-2086. https://doi.org/10.3892/ijo.2013.1889
MLA
Yang, J., Liu, P., Tian, M., Li, Y., Chen, W., Li, X."Proteomic identification of angiomotin by ProteomeLab PF-2D and correlation with clinical outcome in human clear cell renal cell carcinoma". International Journal of Oncology 42.6 (2013): 2078-2086.
Chicago
Yang, J., Liu, P., Tian, M., Li, Y., Chen, W., Li, X."Proteomic identification of angiomotin by ProteomeLab PF-2D and correlation with clinical outcome in human clear cell renal cell carcinoma". International Journal of Oncology 42, no. 6 (2013): 2078-2086. https://doi.org/10.3892/ijo.2013.1889
Copy and paste a formatted citation
x
Spandidos Publications style
Yang J, Liu P, Tian M, Li Y, Chen W and Li X: Proteomic identification of angiomotin by ProteomeLab PF-2D and correlation with clinical outcome in human clear cell renal cell carcinoma. Int J Oncol 42: 2078-2086, 2013.
APA
Yang, J., Liu, P., Tian, M., Li, Y., Chen, W., & Li, X. (2013). Proteomic identification of angiomotin by ProteomeLab PF-2D and correlation with clinical outcome in human clear cell renal cell carcinoma. International Journal of Oncology, 42, 2078-2086. https://doi.org/10.3892/ijo.2013.1889
MLA
Yang, J., Liu, P., Tian, M., Li, Y., Chen, W., Li, X."Proteomic identification of angiomotin by ProteomeLab PF-2D and correlation with clinical outcome in human clear cell renal cell carcinoma". International Journal of Oncology 42.6 (2013): 2078-2086.
Chicago
Yang, J., Liu, P., Tian, M., Li, Y., Chen, W., Li, X."Proteomic identification of angiomotin by ProteomeLab PF-2D and correlation with clinical outcome in human clear cell renal cell carcinoma". International Journal of Oncology 42, no. 6 (2013): 2078-2086. https://doi.org/10.3892/ijo.2013.1889
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