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Article

Regulation of the expression of CLU isoforms in endometrial proliferative diseases

  • Authors:
    • Paolo Fuzio
    • Alessio Valletti
    • Anna Napoli
    • Grazia Napoli
    • Gennaro Cormio
    • Luigi Selvaggi
    • Sabino Liuni
    • Graziano Pesole
    • Eugenio Maiorano
    • Elda Perlino
  • View Affiliations / Copyright

    Affiliations: Institute of Biomedical Technologies, ITB-CNR, I-70126 Bari, Italy, Institute of Biomembranes and Bioenergetics, IBBE‑CNR, I-70126 Bari, Italy, Interdisciplinary Department of Medicine, University of Bari ‘Aldo Moro’, I-70124 Bari, Italy, Department of Pathological Anatomy and Genetics, Hospital of SS. Annunziata, I-74100 Taranto, Italy, Department of Biomedical Sciences and Human Oncology, DIMO, University of Bari ‘Aldo Moro’, I-70124 Bari, Italy
  • Pages: 1929-1944
    |
    Published online on: April 11, 2013
       https://doi.org/10.3892/ijo.2013.1894
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Abstract

Clusterin (CLU) is a nearly ubiquitous multifunctional protein synthesized in different functionally divergent isoforms, sCLU and nCLU, playing a crucial role by keeping a balance between cell proliferation and death. Studying in vivo CLU expression we found a higher mRNA expression both in neoplastic and hyperplastic tissues in comparison to normal endometria; in particular, by RT-qPCR we demonstrated an increase of the specific sCLU isoform in the neoplastic and hyperplastic endometrial diseases. On the contrary, no CLU increase was detected at the protein level. The CLU gene transcriptional activity was upregulated in the hyperplastic and neoplastic tissues, indicating the existence of a fine post-trans­criptional regulation of CLU expression possibly responsible for the protein decrease in the malignant disease. A specific CLU immunoreactivity was present in all the endometrial glandular cells in comparison to the other cellular compartments where CLU immunoreactivity was lower or absent. In conclusion, our results suggest the existence of a complex regulatory mechanism of CLU gene expression during the progression from normal to malignant cells, possibly contributing to endometrial carcinogenesis. Moreover, the specific alteration of the sCLU:nCLU ratio associated with the pathological stage, suggests a possible usage of CLU as molecular biomarker for the diagnosis/prognosis of endometrial proliferative diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Fuzio P, Valletti A, Napoli A, Napoli G, Cormio G, Selvaggi L, Liuni S, Pesole G, Maiorano E, Perlino E, Perlino E, et al: Regulation of the expression of CLU isoforms in endometrial proliferative diseases. Int J Oncol 42: 1929-1944, 2013.
APA
Fuzio, P., Valletti, A., Napoli, A., Napoli, G., Cormio, G., Selvaggi, L. ... Perlino, E. (2013). Regulation of the expression of CLU isoforms in endometrial proliferative diseases. International Journal of Oncology, 42, 1929-1944. https://doi.org/10.3892/ijo.2013.1894
MLA
Fuzio, P., Valletti, A., Napoli, A., Napoli, G., Cormio, G., Selvaggi, L., Liuni, S., Pesole, G., Maiorano, E., Perlino, E."Regulation of the expression of CLU isoforms in endometrial proliferative diseases". International Journal of Oncology 42.6 (2013): 1929-1944.
Chicago
Fuzio, P., Valletti, A., Napoli, A., Napoli, G., Cormio, G., Selvaggi, L., Liuni, S., Pesole, G., Maiorano, E., Perlino, E."Regulation of the expression of CLU isoforms in endometrial proliferative diseases". International Journal of Oncology 42, no. 6 (2013): 1929-1944. https://doi.org/10.3892/ijo.2013.1894
Copy and paste a formatted citation
x
Spandidos Publications style
Fuzio P, Valletti A, Napoli A, Napoli G, Cormio G, Selvaggi L, Liuni S, Pesole G, Maiorano E, Perlino E, Perlino E, et al: Regulation of the expression of CLU isoforms in endometrial proliferative diseases. Int J Oncol 42: 1929-1944, 2013.
APA
Fuzio, P., Valletti, A., Napoli, A., Napoli, G., Cormio, G., Selvaggi, L. ... Perlino, E. (2013). Regulation of the expression of CLU isoforms in endometrial proliferative diseases. International Journal of Oncology, 42, 1929-1944. https://doi.org/10.3892/ijo.2013.1894
MLA
Fuzio, P., Valletti, A., Napoli, A., Napoli, G., Cormio, G., Selvaggi, L., Liuni, S., Pesole, G., Maiorano, E., Perlino, E."Regulation of the expression of CLU isoforms in endometrial proliferative diseases". International Journal of Oncology 42.6 (2013): 1929-1944.
Chicago
Fuzio, P., Valletti, A., Napoli, A., Napoli, G., Cormio, G., Selvaggi, L., Liuni, S., Pesole, G., Maiorano, E., Perlino, E."Regulation of the expression of CLU isoforms in endometrial proliferative diseases". International Journal of Oncology 42, no. 6 (2013): 1929-1944. https://doi.org/10.3892/ijo.2013.1894
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