In vitro and in vivo growth inhibition of prostate cancer by the small molecule imiquimod

  • Authors:
    • Ju-Hee Han
    • Junglim Lee
    • Soo-Jin Jeon
    • Eun-Sun Choi
    • Sung-Dae Cho
    • Bo-Yeon Kim
    • Dong-Jae Kim
    • Jae-Hak Park
    • Jong-Hwan Park
  • View Affiliations

  • Published online on: April 16, 2013     https://doi.org/10.3892/ijo.2013.1898
  • Pages: 2087-2093
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Abstract

Prostate cancer is the second leading cause of cancer death in men worldwide. In the present study, we examined in vitro and in vivo antitumor effect of the small molecule imiquimod, also known as a TLR7 agonist, against prostate cancer. Imiquimod inhibited the growth of mouse (TRAMP‑C2) and human (PC-3) prostate cancer cells. Treatment with imiquimod induced cell cycle arrest at the G2/M phase in TRMPA-C2 cells, confirmed by the changes of G2/M checkpoint regulators such as reduction of cyclin B1 expression and increase of phospho-CDC2 and p21 in TRAMP-C2 cells treated with imiquimod. Flow cytometry and western blot analysis revealed that imiquimod induced direct apoptosis in TRAMP-C2 cells via a mitochondrial‑dependent pathway. Intratumoral injection with imiquimod reduced significantly tumor growth and increased apoptotic cells in mice subcutaneously implanted with TRAMP-C2 cells. Our results indicate that imiquimod can be an alternative therapeutic for locally generated prostate cancer.
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June 2013
Volume 42 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Han J, Lee J, Jeon S, Choi E, Cho S, Kim B, Kim D, Park J and Park J: In vitro and in vivo growth inhibition of prostate cancer by the small molecule imiquimod. Int J Oncol 42: 2087-2093, 2013
APA
Han, J., Lee, J., Jeon, S., Choi, E., Cho, S., Kim, B. ... Park, J. (2013). In vitro and in vivo growth inhibition of prostate cancer by the small molecule imiquimod. International Journal of Oncology, 42, 2087-2093. https://doi.org/10.3892/ijo.2013.1898
MLA
Han, J., Lee, J., Jeon, S., Choi, E., Cho, S., Kim, B., Kim, D., Park, J., Park, J."In vitro and in vivo growth inhibition of prostate cancer by the small molecule imiquimod". International Journal of Oncology 42.6 (2013): 2087-2093.
Chicago
Han, J., Lee, J., Jeon, S., Choi, E., Cho, S., Kim, B., Kim, D., Park, J., Park, J."In vitro and in vivo growth inhibition of prostate cancer by the small molecule imiquimod". International Journal of Oncology 42, no. 6 (2013): 2087-2093. https://doi.org/10.3892/ijo.2013.1898