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Article

Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: Nuclear HO-1 promotes vascular endothelial growth factor secretion

  • Authors:
    • Gabriel Birrane
    • Huchun Li
    • Suping Yang
    • Souvenir D. Tachado
    • Seyha Seng
  • View Affiliations / Copyright

    Affiliations: Division of Experimental Medicine, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA, Division of Molecular and Vascular Medicine, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
  • Pages: 1919-1928
    |
    Published online on: April 17, 2013
       https://doi.org/10.3892/ijo.2013.1910
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Abstract

Prostate cancer is the second leading cause of male-cancer related death in the United States. Despite a number of evidence-based studies which strongly suggest an association between cigarette smoking and prostate cancer, the underlying biological mechanism is largely unknown. Heme oxygenase 1 (HO-1) has been implicated in maintaining cellular homeostasis, but also in tumor angiogenesis. Nuclear HO-1 protein expression has been observed in various types of tumors including prostate cancer. These studies, however, were reported as clinical and pathological observations, and failed to investigate nuclear HO-1 at the molecular level in cancer. The present study explores the relationship between cigarette smoke and nuclear HO-1-modulated promotion of vascular endothelial growth factor (VEGF) secretion. We have demonstrated that cigarette smoke medium (SM)-induced HO-1 mRNA expression and upregulated HO-1 protein levels in the prostate cancer cell lines DU145 and PC3. We also observed that SM significantly induced nuclear expression of HO-1, and enhanced secretion of VEGF in cells. Nuclear-directed expression of HO-1 activated the transcriptional activity of VEGF and promoted VEGF secretion in prostate cancer cells. This study provides new insights into the molecular mechanism by which cigarette smoke-induced nuclear translocation of HO-1 promotes VEGF secretion in prostate cancer cells. Nuclear HO-1 may, therefore, constitute an attractive therapeutic target to inhibit angiogenesis and the progression of prostate cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Birrane G, Li H, Yang S, Tachado SD and Seng S: Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: Nuclear HO-1 promotes vascular endothelial growth factor secretion. Int J Oncol 42: 1919-1928, 2013.
APA
Birrane, G., Li, H., Yang, S., Tachado, S.D., & Seng, S. (2013). Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: Nuclear HO-1 promotes vascular endothelial growth factor secretion. International Journal of Oncology, 42, 1919-1928. https://doi.org/10.3892/ijo.2013.1910
MLA
Birrane, G., Li, H., Yang, S., Tachado, S. D., Seng, S."Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: Nuclear HO-1 promotes vascular endothelial growth factor secretion". International Journal of Oncology 42.6 (2013): 1919-1928.
Chicago
Birrane, G., Li, H., Yang, S., Tachado, S. D., Seng, S."Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: Nuclear HO-1 promotes vascular endothelial growth factor secretion". International Journal of Oncology 42, no. 6 (2013): 1919-1928. https://doi.org/10.3892/ijo.2013.1910
Copy and paste a formatted citation
x
Spandidos Publications style
Birrane G, Li H, Yang S, Tachado SD and Seng S: Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: Nuclear HO-1 promotes vascular endothelial growth factor secretion. Int J Oncol 42: 1919-1928, 2013.
APA
Birrane, G., Li, H., Yang, S., Tachado, S.D., & Seng, S. (2013). Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: Nuclear HO-1 promotes vascular endothelial growth factor secretion. International Journal of Oncology, 42, 1919-1928. https://doi.org/10.3892/ijo.2013.1910
MLA
Birrane, G., Li, H., Yang, S., Tachado, S. D., Seng, S."Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: Nuclear HO-1 promotes vascular endothelial growth factor secretion". International Journal of Oncology 42.6 (2013): 1919-1928.
Chicago
Birrane, G., Li, H., Yang, S., Tachado, S. D., Seng, S."Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: Nuclear HO-1 promotes vascular endothelial growth factor secretion". International Journal of Oncology 42, no. 6 (2013): 1919-1928. https://doi.org/10.3892/ijo.2013.1910
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