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International Journal of Oncology
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Article

Epithelial-mesenchymal transition triggers cancer stem cell generation in human thyroid cancer cells

  • Authors:
    • Ling Lan
    • Yong Luo
    • Dai Cui
    • Bing-Yin Shi
    • Wei Deng
    • Li-Li Huo
    • Hai-Ling Chen
    • Guo-Ying Zhang
    • Li-Li Deng
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Beijing Ji Shui Tan Hospital, The 4th Medical College of Peking University, Beijing, P.R. China, Department of Urology, Affiliated Beijing Anzhen Hospital of Capital Medical University, Beijing, P.R. China, Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University, Nanjing, P.R. China, Department of Endocrinology, The First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, P.R. China
  • Pages: 113-120
    |
    Published online on: April 22, 2013
       https://doi.org/10.3892/ijo.2013.1913
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Abstract

Increasing evidence has shown that cancer stem cells or tumor initiating cells are the ‘root cause’ of malignant cancers. However, the exact origin of cancer stem cells still remains obscure in thyroid research. EMT has been implicated in the initiation and conversion of early-stage tumors into invasive malignancies and is associated with the stemness of cancer cells. Based on these facts, a new hypothesis was suggested that EMT induces cancer stem cell generation and tumor progression in human thyroid cancer cells in vitro. In the present study, FTC133 cells identified as EMT-negative cells were used for EMT induction by HIF‑1α transfection. Overexpression of HIF-1α induced FTC133 cells to undergo EMT, downregulated the epithelial markers E-cadherin, upregulated the mesenchymal marker vimentin, and associated with highly invasive and metastatic properties. Most importantly, the induction of EMT promoted the stem-like side population cell proportion in the FTC133 cells. These results indicate that EMT induction promotes CSC traits and cell proportions in the thyroid cancer cells, which implies that EMT could induce cancer stem cell generation and tumor progression in thyroid cancers. Further understanding of the role of EMT and cancer stem cells in cancer progression may reveal new targets for the prevention or therapy of thyroid cancers.
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Copy and paste a formatted citation
Spandidos Publications style
Lan L, Luo Y, Cui D, Shi B, Deng W, Huo L, Chen H, Zhang G and Deng L: Epithelial-mesenchymal transition triggers cancer stem cell generation in human thyroid cancer cells. Int J Oncol 43: 113-120, 2013.
APA
Lan, L., Luo, Y., Cui, D., Shi, B., Deng, W., Huo, L. ... Deng, L. (2013). Epithelial-mesenchymal transition triggers cancer stem cell generation in human thyroid cancer cells. International Journal of Oncology, 43, 113-120. https://doi.org/10.3892/ijo.2013.1913
MLA
Lan, L., Luo, Y., Cui, D., Shi, B., Deng, W., Huo, L., Chen, H., Zhang, G., Deng, L."Epithelial-mesenchymal transition triggers cancer stem cell generation in human thyroid cancer cells". International Journal of Oncology 43.1 (2013): 113-120.
Chicago
Lan, L., Luo, Y., Cui, D., Shi, B., Deng, W., Huo, L., Chen, H., Zhang, G., Deng, L."Epithelial-mesenchymal transition triggers cancer stem cell generation in human thyroid cancer cells". International Journal of Oncology 43, no. 1 (2013): 113-120. https://doi.org/10.3892/ijo.2013.1913
Copy and paste a formatted citation
x
Spandidos Publications style
Lan L, Luo Y, Cui D, Shi B, Deng W, Huo L, Chen H, Zhang G and Deng L: Epithelial-mesenchymal transition triggers cancer stem cell generation in human thyroid cancer cells. Int J Oncol 43: 113-120, 2013.
APA
Lan, L., Luo, Y., Cui, D., Shi, B., Deng, W., Huo, L. ... Deng, L. (2013). Epithelial-mesenchymal transition triggers cancer stem cell generation in human thyroid cancer cells. International Journal of Oncology, 43, 113-120. https://doi.org/10.3892/ijo.2013.1913
MLA
Lan, L., Luo, Y., Cui, D., Shi, B., Deng, W., Huo, L., Chen, H., Zhang, G., Deng, L."Epithelial-mesenchymal transition triggers cancer stem cell generation in human thyroid cancer cells". International Journal of Oncology 43.1 (2013): 113-120.
Chicago
Lan, L., Luo, Y., Cui, D., Shi, B., Deng, W., Huo, L., Chen, H., Zhang, G., Deng, L."Epithelial-mesenchymal transition triggers cancer stem cell generation in human thyroid cancer cells". International Journal of Oncology 43, no. 1 (2013): 113-120. https://doi.org/10.3892/ijo.2013.1913
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