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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Article

Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter

  • Authors:
    • Yun Hua Li
    • Li Fang Jin
    • Lian-Fang Du
    • Qiu Sheng Shi
    • Long Liu
    • Xiao Jia
    • Ying Wu
    • Fan Li
    • Hang Hui Wang
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasound, Shanghai First People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China
  • Pages: 151-158
    |
    Published online on: April 25, 2013
       https://doi.org/10.3892/ijo.2013.1921
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Abstract

Gene therapy is a potentially viable approach for treating hormone-refractory prostate cancer (HRPC), it requires efficient delivery systems and a target gene. Inducing carcinoma cell apoptosis by inhibition of heat shock protein 70 (HSP70) overexpression has been emerging as an attractive strategy for cancer therapy. In our study, the high tumor-specificity of human telomerase reverse transcriptase (HTERT) expression prompted the use of an HTERT/cytomegalovirus (CMV) chimeric promoter to drive HSP70-ShRNA expression to induce HRPC 22RV1 cell apoptosis. At the same time, sonoporation induced by ultrasound-targeted microbubble destruction (UTMD) was utilized for delivery of plasmid loaded with HTERT/CMV promoter. Our results indicated the combination of sonoporation, low-dose liposomes and HTERT/CMV chimeric promoter as a delivery system has the potential to promote efficient gene transfer with lower cytotoxicity.
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Copy and paste a formatted citation
Spandidos Publications style
Li YH, Jin LF, Du L, Shi QS, Liu L, Jia X, Wu Y, Li F and Wang HH: Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter. Int J Oncol 43: 151-158, 2013.
APA
Li, Y.H., Jin, L.F., Du, L., Shi, Q.S., Liu, L., Jia, X. ... Wang, H.H. (2013). Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter. International Journal of Oncology, 43, 151-158. https://doi.org/10.3892/ijo.2013.1921
MLA
Li, Y. H., Jin, L. F., Du, L., Shi, Q. S., Liu, L., Jia, X., Wu, Y., Li, F., Wang, H. H."Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter". International Journal of Oncology 43.1 (2013): 151-158.
Chicago
Li, Y. H., Jin, L. F., Du, L., Shi, Q. S., Liu, L., Jia, X., Wu, Y., Li, F., Wang, H. H."Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter". International Journal of Oncology 43, no. 1 (2013): 151-158. https://doi.org/10.3892/ijo.2013.1921
Copy and paste a formatted citation
x
Spandidos Publications style
Li YH, Jin LF, Du L, Shi QS, Liu L, Jia X, Wu Y, Li F and Wang HH: Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter. Int J Oncol 43: 151-158, 2013.
APA
Li, Y.H., Jin, L.F., Du, L., Shi, Q.S., Liu, L., Jia, X. ... Wang, H.H. (2013). Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter. International Journal of Oncology, 43, 151-158. https://doi.org/10.3892/ijo.2013.1921
MLA
Li, Y. H., Jin, L. F., Du, L., Shi, Q. S., Liu, L., Jia, X., Wu, Y., Li, F., Wang, H. H."Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter". International Journal of Oncology 43.1 (2013): 151-158.
Chicago
Li, Y. H., Jin, L. F., Du, L., Shi, Q. S., Liu, L., Jia, X., Wu, Y., Li, F., Wang, H. H."Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter". International Journal of Oncology 43, no. 1 (2013): 151-158. https://doi.org/10.3892/ijo.2013.1921
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