Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter

Li,Yun Hua; Jin,Li Fang; Du,Lian-Fang; Shi,Qiu  Sheng; Liu,Long; Jia,Xiao; Wu,Ying; Li,Fan; Wang,Hang Hui

doi:10.3892/ijo.2013.1921

Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter

Authors:
- Yun Hua Li
- Li Fang Jin
- Lian-Fang Du
- Qiu Sheng Shi
- Long Liu
- Xiao Jia
- Ying Wu
- Fan Li
- Hang Hui Wang
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Affiliations: Department of Ultrasound, Shanghai First People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China
Published online on: April 25, 2013 https://doi.org/10.3892/ijo.2013.1921
Pages: 151-158

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Abstract

Gene therapy is a potentially viable approach for treating hormone-refractory prostate cancer (HRPC), it requires efficient delivery systems and a target gene. Inducing carcinoma cell apoptosis by inhibition of heat shock protein 70 (HSP70) overexpression has been emerging as an attractive strategy for cancer therapy. In our study, the high tumor-specificity of human telomerase reverse transcriptase (HTERT) expression prompted the use of an HTERT/cytomegalovirus (CMV) chimeric promoter to drive HSP70-ShRNA expression to induce HRPC 22RV1 cell apoptosis. At the same time, sonoporation induced by ultrasound-targeted microbubble destruction (UTMD) was utilized for delivery of plasmid loaded with HTERT/CMV promoter. Our results indicated the combination of sonoporation, low-dose liposomes and HTERT/CMV chimeric promoter as a delivery system has the potential to promote efficient gene transfer with lower cytotoxicity.

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