Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML

  • Authors:
    • Gianni Capalbo
    • Thea Mueller-Kuller
    • Steffen Koschmieder
    • Hans-Ulrich Klein
    • Oliver G. Ottmann
    • Dieter Hoelzer
    • Urban J. Scheuring
  • View Affiliations

  • Published online on: April 29, 2013     https://doi.org/10.3892/ijo.2013.1924
  • Pages: 246-254
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Abstract

The gene product of the zinc finger CCCH-type containing 15 (ZC3H15) gene, an immediate early erythropoietin response gene (synonymous: LEREPO4), was further characterized. ZC3H15 was expressed ubiquitously in all human tissues tested by northern blotting and showed mainly a diffuse cytoplasmic distribution by immune fluorescence microscopy and western blotting of subcellular protein fractions. The expression of ZC3H15 was downregulated effectively in HeLa cells to ≤13% of the control by transfection of specific small interfering RNA (siRNA). Subsequent Affymetrix microarray analysis revealed 202 differentially expressed genes including 114 induced (≥3-fold) genes and 88 suppressed (≤0.3-fold) genes. The gene ontology (GO) categories containing an over-representation of differentially expressed genes comprised cell growth, transcription, cell adhesion, regulation of NF-κB, regulation of MAPK, cell cycle arrest and immune response. ZC3H15 interacted with the signaling adapter protein tumor necrosis factor receptor associated factor 2 (TRAF-2) as shown by co-immunoprecipitation. ZC3H15 expression was found to be significantly increased in acute myeloid leukemia (AML) samples compared to MDS, CML, ALL and normal bone marrow samples using the Leukemia Gene Atlas (LGA) database. Based on these data, it is hypothesized that ZC3H15 may interact with TRAF-2 functionally within the NF-κB pathway, and may be explored as a potential target in AML.
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July 2013
Volume 43 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Capalbo G, Mueller-Kuller T, Koschmieder S, Klein H, Ottmann OG, Hoelzer D and Scheuring UJ: Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML. Int J Oncol 43: 246-254, 2013
APA
Capalbo, G., Mueller-Kuller, T., Koschmieder, S., Klein, H., Ottmann, O.G., Hoelzer, D., & Scheuring, U.J. (2013). Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML. International Journal of Oncology, 43, 246-254. https://doi.org/10.3892/ijo.2013.1924
MLA
Capalbo, G., Mueller-Kuller, T., Koschmieder, S., Klein, H., Ottmann, O. G., Hoelzer, D., Scheuring, U. J."Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML". International Journal of Oncology 43.1 (2013): 246-254.
Chicago
Capalbo, G., Mueller-Kuller, T., Koschmieder, S., Klein, H., Ottmann, O. G., Hoelzer, D., Scheuring, U. J."Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML". International Journal of Oncology 43, no. 1 (2013): 246-254. https://doi.org/10.3892/ijo.2013.1924