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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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July 2013 Volume 43 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML

  • Authors:
    • Gianni Capalbo
    • Thea Mueller-Kuller
    • Steffen Koschmieder
    • Hans-Ulrich Klein
    • Oliver G. Ottmann
    • Dieter Hoelzer
    • Urban J. Scheuring
  • View Affiliations / Copyright

    Affiliations: Department of Hematology/Oncology and Infectious Diseases, J.W. Goethe-University Hospital, 60595 Frankfurt/Main, Germany, Department of Medicine (Oncology, Hematology and Stem Cell Transplantation), University Medical Center of Aachen, 52074 Aachen, Germany, Institute of Medical Informatics, University of Münster, 48149 Münster, Germany
  • Pages: 246-254
    |
    Published online on: April 29, 2013
       https://doi.org/10.3892/ijo.2013.1924
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Abstract

The gene product of the zinc finger CCCH-type containing 15 (ZC3H15) gene, an immediate early erythropoietin response gene (synonymous: LEREPO4), was further characterized. ZC3H15 was expressed ubiquitously in all human tissues tested by northern blotting and showed mainly a diffuse cytoplasmic distribution by immune fluorescence microscopy and western blotting of subcellular protein fractions. The expression of ZC3H15 was downregulated effectively in HeLa cells to ≤13% of the control by transfection of specific small interfering RNA (siRNA). Subsequent Affymetrix microarray analysis revealed 202 differentially expressed genes including 114 induced (≥3-fold) genes and 88 suppressed (≤0.3-fold) genes. The gene ontology (GO) categories containing an over-representation of differentially expressed genes comprised cell growth, transcription, cell adhesion, regulation of NF-κB, regulation of MAPK, cell cycle arrest and immune response. ZC3H15 interacted with the signaling adapter protein tumor necrosis factor receptor associated factor 2 (TRAF-2) as shown by co-immunoprecipitation. ZC3H15 expression was found to be significantly increased in acute myeloid leukemia (AML) samples compared to MDS, CML, ALL and normal bone marrow samples using the Leukemia Gene Atlas (LGA) database. Based on these data, it is hypothesized that ZC3H15 may interact with TRAF-2 functionally within the NF-κB pathway, and may be explored as a potential target in AML.
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Copy and paste a formatted citation
Spandidos Publications style
Capalbo G, Mueller-Kuller T, Koschmieder S, Klein H, Ottmann OG, Hoelzer D and Scheuring UJ: Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML. Int J Oncol 43: 246-254, 2013.
APA
Capalbo, G., Mueller-Kuller, T., Koschmieder, S., Klein, H., Ottmann, O.G., Hoelzer, D., & Scheuring, U.J. (2013). Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML. International Journal of Oncology, 43, 246-254. https://doi.org/10.3892/ijo.2013.1924
MLA
Capalbo, G., Mueller-Kuller, T., Koschmieder, S., Klein, H., Ottmann, O. G., Hoelzer, D., Scheuring, U. J."Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML". International Journal of Oncology 43.1 (2013): 246-254.
Chicago
Capalbo, G., Mueller-Kuller, T., Koschmieder, S., Klein, H., Ottmann, O. G., Hoelzer, D., Scheuring, U. J."Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML". International Journal of Oncology 43, no. 1 (2013): 246-254. https://doi.org/10.3892/ijo.2013.1924
Copy and paste a formatted citation
x
Spandidos Publications style
Capalbo G, Mueller-Kuller T, Koschmieder S, Klein H, Ottmann OG, Hoelzer D and Scheuring UJ: Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML. Int J Oncol 43: 246-254, 2013.
APA
Capalbo, G., Mueller-Kuller, T., Koschmieder, S., Klein, H., Ottmann, O.G., Hoelzer, D., & Scheuring, U.J. (2013). Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML. International Journal of Oncology, 43, 246-254. https://doi.org/10.3892/ijo.2013.1924
MLA
Capalbo, G., Mueller-Kuller, T., Koschmieder, S., Klein, H., Ottmann, O. G., Hoelzer, D., Scheuring, U. J."Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML". International Journal of Oncology 43.1 (2013): 246-254.
Chicago
Capalbo, G., Mueller-Kuller, T., Koschmieder, S., Klein, H., Ottmann, O. G., Hoelzer, D., Scheuring, U. J."Characterization of ZC3H15 as a potential TRAF-2-interacting protein implicated in the NFκB pathway and overexpressed in AML". International Journal of Oncology 43, no. 1 (2013): 246-254. https://doi.org/10.3892/ijo.2013.1924
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