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Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation

  • Authors:
    • Ulrike Fischer
    • Stefanie Rheinheimer
    • Andrea Krempler
    • Markus Löbrich
    • Eckart Meese
  • View Affiliations / Copyright

    Affiliations: Department of Human Genetics, Medical School, Saarland University, D-66421 Homburg/Saar, Germany, Radiation Biology and DNA Repair, Darmstadt University of Technology, D-64287 Darmstadt, Germany
    Copyright: © Fischer et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 50-56
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    Published online on: May 13, 2013
       https://doi.org/10.3892/ijo.2013.1937
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Abstract

Human glioblastomas are characterized by frequent DNA amplifications most often at chromosome regions 7p11.2 and 12q13-15. Although amplification is a well-known hallmark of glioblastoma genetics the function of most amplified genes in glioblastoma biology is not understood. Previously, we cloned Ku70-binding protein 3 (KUB3) from the amplified domain at 12q13-15. Here, we report that glioblastoma cell cultures with endogenous KUB3 gene amplification and with elevated KUB3 protein expression show an efficient double-strand break (DSB) repair after being irradiated with 1 Gy. A significantly less efficient DSB repair was found in glioma cell cultures without KUB3 amplification and expression. Furthermore, we found that a siRNA-mediated reduction of the endogenous KUB3 expression in glioblastoma cells resulted in a reduction of the repair efficiency. HeLa cells transfected with KUB3 showed an increased DSB repair in comparison to untreated HeLa cells. In addition, KUB3 seems to influence DSB efficiency via the DNA-PK-dependent repair pathway as shown by simultaneous inhibition of KUB3 and DNA-PK. The data provide the first evidence for a link between the level of KUB3 amplification and expression in glioma and DSB repair efficiency.
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Copy and paste a formatted citation
Spandidos Publications style
Fischer U, Rheinheimer S, Krempler A, Löbrich M and Meese E: Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation. Int J Oncol 43: 50-56, 2013.
APA
Fischer, U., Rheinheimer, S., Krempler, A., Löbrich, M., & Meese, E. (2013). Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation. International Journal of Oncology, 43, 50-56. https://doi.org/10.3892/ijo.2013.1937
MLA
Fischer, U., Rheinheimer, S., Krempler, A., Löbrich, M., Meese, E."Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation". International Journal of Oncology 43.1 (2013): 50-56.
Chicago
Fischer, U., Rheinheimer, S., Krempler, A., Löbrich, M., Meese, E."Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation". International Journal of Oncology 43, no. 1 (2013): 50-56. https://doi.org/10.3892/ijo.2013.1937
Copy and paste a formatted citation
x
Spandidos Publications style
Fischer U, Rheinheimer S, Krempler A, Löbrich M and Meese E: Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation. Int J Oncol 43: 50-56, 2013.
APA
Fischer, U., Rheinheimer, S., Krempler, A., Löbrich, M., & Meese, E. (2013). Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation. International Journal of Oncology, 43, 50-56. https://doi.org/10.3892/ijo.2013.1937
MLA
Fischer, U., Rheinheimer, S., Krempler, A., Löbrich, M., Meese, E."Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation". International Journal of Oncology 43.1 (2013): 50-56.
Chicago
Fischer, U., Rheinheimer, S., Krempler, A., Löbrich, M., Meese, E."Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation". International Journal of Oncology 43, no. 1 (2013): 50-56. https://doi.org/10.3892/ijo.2013.1937
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