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Article

Trop2 regulates the proliferation and differentiation of murine compact-bone derived MSCs

  • Authors:
    • Jianye Yang
    • Zhaohui Zhu
    • Hongfei Wang
    • Feifei Li
    • Xinling Du
    • Runlin Z. Ma
  • View Affiliations / Copyright

    Affiliations: Department of Cardio-Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, State Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R. China, Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
  • Pages: 859-867
    |
    Published online on: June 19, 2013
       https://doi.org/10.3892/ijo.2013.1987
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Abstract

Mesenchymal stem cells (MSCs) show promising potential in cell-based therapies for wound repair and tissue engineering. However, their applications are hindered by our limited understanding of the detailed regulation of the cell fates in vitro and in vivo. To investigate the relationship between MSCs and Trop2, a protein overexpressed in cancer cells, we constructed a Trop2 gene knockout mouse by homologous recombination. Herein, we report that Trop2 is expressed exclusively on the MSC membrane and is involved in the regulation of proliferation and differentiation of MSCs. The proliferation of MSCs in Trop2 KO mice was significantly inhibited with prolonged cellular doubling time and reduced cell numbers entering the S phase. Trop2 deficiency impaired the differentiation of MSCs by significant reduction of adipogenesis and osteogenesis. AKT activation was impaired and cyclin D1 expression was downregulated in the Trop2-deficient MSCs, resulting in significant delay in cell cycle progression and inhibition of MSC self-renewal. Collectively, our findings help to establish a close functional link between Trop2 and MSCs, and to provide a tool for future investigation of Trop2 in other types of stem cells.
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Copy and paste a formatted citation
Spandidos Publications style
Yang J, Zhu Z, Wang H, Li F, Du X and Ma RZ: Trop2 regulates the proliferation and differentiation of murine compact-bone derived MSCs. Int J Oncol 43: 859-867, 2013.
APA
Yang, J., Zhu, Z., Wang, H., Li, F., Du, X., & Ma, R.Z. (2013). Trop2 regulates the proliferation and differentiation of murine compact-bone derived MSCs. International Journal of Oncology, 43, 859-867. https://doi.org/10.3892/ijo.2013.1987
MLA
Yang, J., Zhu, Z., Wang, H., Li, F., Du, X., Ma, R. Z."Trop2 regulates the proliferation and differentiation of murine compact-bone derived MSCs". International Journal of Oncology 43.3 (2013): 859-867.
Chicago
Yang, J., Zhu, Z., Wang, H., Li, F., Du, X., Ma, R. Z."Trop2 regulates the proliferation and differentiation of murine compact-bone derived MSCs". International Journal of Oncology 43, no. 3 (2013): 859-867. https://doi.org/10.3892/ijo.2013.1987
Copy and paste a formatted citation
x
Spandidos Publications style
Yang J, Zhu Z, Wang H, Li F, Du X and Ma RZ: Trop2 regulates the proliferation and differentiation of murine compact-bone derived MSCs. Int J Oncol 43: 859-867, 2013.
APA
Yang, J., Zhu, Z., Wang, H., Li, F., Du, X., & Ma, R.Z. (2013). Trop2 regulates the proliferation and differentiation of murine compact-bone derived MSCs. International Journal of Oncology, 43, 859-867. https://doi.org/10.3892/ijo.2013.1987
MLA
Yang, J., Zhu, Z., Wang, H., Li, F., Du, X., Ma, R. Z."Trop2 regulates the proliferation and differentiation of murine compact-bone derived MSCs". International Journal of Oncology 43.3 (2013): 859-867.
Chicago
Yang, J., Zhu, Z., Wang, H., Li, F., Du, X., Ma, R. Z."Trop2 regulates the proliferation and differentiation of murine compact-bone derived MSCs". International Journal of Oncology 43, no. 3 (2013): 859-867. https://doi.org/10.3892/ijo.2013.1987
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