Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression

Kawamoto,Yukihiro; Ohyama,Yoshito; Chiba,Tadashige; Yagishita,Hisao; Sakashita,Hideaki; Imai,Kazushi

doi:10.3892/ijo.2013.1990

Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression

Authors:
- Yukihiro Kawamoto
- Yoshito Ohyama
- Tadashige Chiba
- Hisao Yagishita
- Hideaki Sakashita
- Kazushi Imai
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Affiliations: Division of Oral and Maxillofacial Surgery 2, Department of Diagnostic and Therapeutic Sciences, School of Dentistry, Meikai University, Saitama, Japan, Department of Biochemistry, School of Life Dentistry at Tokyo, The Nippon Dental University, Tokyo, Japan, The Nippon Dental University Hospital, The Nippon Dental University, Tokyo, Japan
Published online on: June 25, 2013 https://doi.org/10.3892/ijo.2013.1990
Pages: 729-736

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Abstract

Progression of oral carcinomas associates with aberrant activation and inactivation of molecules that work in established or unknown pathways. Although mucosa‑associated lymphoid tissue 1 (MALT1) expressed in normal oral epithelium is inactivated in the aggressive subset of carcinomas with worse prognosis, phenotypic changes of carcinoma cells upon the loss of expression is unknown. We performed a proteomic analysis to identify MALT1‑regulated proteins in oral carcinoma cells. Four different keratins were included in the ten most abundantly changed proteins. K8/18 were upregulated in MALT1 stably‑expressing carcinoma cells and K5/14 in MALT1‑marginal control cells. K8/18 upregulation and K5/14 downregulation were MALT1 dose‑dependent and observed in a series of oral carcinoma cells. MALT1 suppressed cell proliferation (0.52-fold, P<0.01) and its dominant-negative form stimulated it (1.33-fold, P<0.01). The decreased proliferation associated with reduction of cyclin D1, which was recovered by the short interfering RNA against MALT1. Taken together, loss of MALT1 expression alters keratin expression and enhances proliferation of carcinoma cells, and may progress oral carcinomas into the advanced state.

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