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Article

Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression

  • Authors:
    • Yukihiro Kawamoto
    • Yoshito Ohyama
    • Tadashige Chiba
    • Hisao Yagishita
    • Hideaki Sakashita
    • Kazushi Imai
  • View Affiliations / Copyright

    Affiliations: Division of Oral and Maxillofacial Surgery 2, Department of Diagnostic and Therapeutic Sciences, School of Dentistry, Meikai University, Saitama, Japan, Department of Biochemistry, School of Life Dentistry at Tokyo, The Nippon Dental University, Tokyo, Japan, The Nippon Dental University Hospital, The Nippon Dental University, Tokyo, Japan
  • Pages: 729-736
    |
    Published online on: June 25, 2013
       https://doi.org/10.3892/ijo.2013.1990
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Abstract

Progression of oral carcinomas associates with aberrant activation and inactivation of molecules that work in established or unknown pathways. Although mucosa‑associated lymphoid tissue 1 (MALT1) expressed in normal oral epithelium is inactivated in the aggressive subset of carcinomas with worse prognosis, phenotypic changes of carcinoma cells upon the loss of expression is unknown. We performed a proteomic analysis to identify MALT1‑regulated proteins in oral carcinoma cells. Four different keratins were included in the ten most abundantly changed proteins. K8/18 were upregulated in MALT1 stably‑expressing carcinoma cells and K5/14 in MALT1‑marginal control cells. K8/18 upregulation and K5/14 downregulation were MALT1 dose‑dependent and observed in a series of oral carcinoma cells. MALT1 suppressed cell proliferation (0.52-fold, P<0.01) and its dominant-negative form stimulated it (1.33-fold, P<0.01). The decreased proliferation associated with reduction of cyclin D1, which was recovered by the short interfering RNA against MALT1. Taken together, loss of MALT1 expression alters keratin expression and enhances proliferation of carcinoma cells, and may progress oral carcinomas into the advanced state.
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Copy and paste a formatted citation
Spandidos Publications style
Kawamoto Y, Ohyama Y, Chiba T, Yagishita H, Sakashita H and Imai K: Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression. Int J Oncol 43: 729-736, 2013.
APA
Kawamoto, Y., Ohyama, Y., Chiba, T., Yagishita, H., Sakashita, H., & Imai, K. (2013). Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression. International Journal of Oncology, 43, 729-736. https://doi.org/10.3892/ijo.2013.1990
MLA
Kawamoto, Y., Ohyama, Y., Chiba, T., Yagishita, H., Sakashita, H., Imai, K."Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression". International Journal of Oncology 43.3 (2013): 729-736.
Chicago
Kawamoto, Y., Ohyama, Y., Chiba, T., Yagishita, H., Sakashita, H., Imai, K."Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression". International Journal of Oncology 43, no. 3 (2013): 729-736. https://doi.org/10.3892/ijo.2013.1990
Copy and paste a formatted citation
x
Spandidos Publications style
Kawamoto Y, Ohyama Y, Chiba T, Yagishita H, Sakashita H and Imai K: Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression. Int J Oncol 43: 729-736, 2013.
APA
Kawamoto, Y., Ohyama, Y., Chiba, T., Yagishita, H., Sakashita, H., & Imai, K. (2013). Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression. International Journal of Oncology, 43, 729-736. https://doi.org/10.3892/ijo.2013.1990
MLA
Kawamoto, Y., Ohyama, Y., Chiba, T., Yagishita, H., Sakashita, H., Imai, K."Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression". International Journal of Oncology 43.3 (2013): 729-736.
Chicago
Kawamoto, Y., Ohyama, Y., Chiba, T., Yagishita, H., Sakashita, H., Imai, K."Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression". International Journal of Oncology 43, no. 3 (2013): 729-736. https://doi.org/10.3892/ijo.2013.1990
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