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Article

Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells

  • Authors:
    • Qian Xu
    • Yusi Tan
    • Kongyan Zhang
    • Yuhong Li
  • View Affiliations / Copyright

    Affiliations: Department of Basic Medicine, Chengde Medical College, Chengde, Hebei 067000, P.R. China
  • Pages: 1187-1193
    |
    Published online on: July 22, 2013
       https://doi.org/10.3892/ijo.2013.2026
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Abstract

Choriocarcinoma is a highly malignant trophoblastic tumor related to pregnancy that often occurs with a complete hydatidiform mole. It grows quickly and can also widely metastasize to other organs or tissues through both the venous and lymphatic systems. The transforming growth factor-β1 (TGF-β1) belongs to a growth factor superfamily and has been suggested to play a critical role in regulating the genesis and development of choriocarcinoma through a variety of Smad-independent pathways, including the p38 MAPK pathway. Previous studies indicated that TGF-β can activate the p38 MAPK pathway. In this study, we investigated Smad and p38 MAPK signaling in JEG-3 choriocarcinoma cells using p38 MAPK inhibitor and TGF-β receptor inhibitor. Immunofluorescence and western blot assays were used to detect the proteins in Smad and p38 MAPK pathways. Our data demonstrated that TGF-β can activate Smad3 and induce Smad3 translocation into the nucleus in JEG-3 cells. Blockade of the TGF-β pathway significantly reduced the expression levels of p38 and phospho-p38. p38 MAPK inhibitors (SB 203580) can attenuate TGF-β1-induced Smad3 expression and suppress the activation of smad3. These findings indicate crosstalk between p38 and smad3 through TGF-β1 in choriocarcinoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Q, Tan Y, Zhang K and Li Y: Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells. Int J Oncol 43: 1187-1193, 2013.
APA
Xu, Q., Tan, Y., Zhang, K., & Li, Y. (2013). Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells. International Journal of Oncology, 43, 1187-1193. https://doi.org/10.3892/ijo.2013.2026
MLA
Xu, Q., Tan, Y., Zhang, K., Li, Y."Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells". International Journal of Oncology 43.4 (2013): 1187-1193.
Chicago
Xu, Q., Tan, Y., Zhang, K., Li, Y."Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells". International Journal of Oncology 43, no. 4 (2013): 1187-1193. https://doi.org/10.3892/ijo.2013.2026
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Q, Tan Y, Zhang K and Li Y: Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells. Int J Oncol 43: 1187-1193, 2013.
APA
Xu, Q., Tan, Y., Zhang, K., & Li, Y. (2013). Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells. International Journal of Oncology, 43, 1187-1193. https://doi.org/10.3892/ijo.2013.2026
MLA
Xu, Q., Tan, Y., Zhang, K., Li, Y."Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells". International Journal of Oncology 43.4 (2013): 1187-1193.
Chicago
Xu, Q., Tan, Y., Zhang, K., Li, Y."Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells". International Journal of Oncology 43, no. 4 (2013): 1187-1193. https://doi.org/10.3892/ijo.2013.2026
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