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Article

Bone formation induced by BMP-2 in human osteosarcoma cells

  • Authors:
    • Lin Wang
    • Paul Park
    • Frank La Marca
    • Khoi Than
    • Shayan Rahman
    • Chia-Ying Lin
  • View Affiliations / Copyright

    Affiliations: Spine Research Laboratory, Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI, USA
  • Pages: 1095-1102
    |
    Published online on: July 23, 2013
       https://doi.org/10.3892/ijo.2013.2030
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Abstract

Our previous studies demonstrated that BMP-2 inhibits the tumorigenicity of cancer stem cells identified as cells with high aldehyde dehydrogenase activity (ALDHbr cells) from the human osteosarcoma cell line OS99-1. We further investigated whether BMP-2 is capable of inducing bone formation in OS99-1 cells. Flow cytometry sorting was used to isolate tumorigenic ALDHbr and non-tumorigenic ALDHlo cells. qRT-PCR was used to quantify the gene expression. A xenograft model was used to verify the bone formation in vivo. There was significantly higher mRNA expression of BMPR1B and BMPR2 in ALDHlo cells compared with that in ALDHbr cells and the BMPR1B expression in ALDHlo cells was ~8-fold higher compared to that in ALDHbr cells. BMP-2 was also found to induce higher transcription of osteogenic markers Runx-2, Osterix (Osx), alkaline phosphatase (ALP) and collagen type I in ALDHlo cells compared to ALDHbr cells, which were mediated by the canonical Smad signaling pathway. In vivo, BMP-2 was identified to induce bone formation in both ALDHbr and ALDHlo cells. All animals receiving 1x104 ALDHlo cells treated with 30 µg of BMP-2 per animal showed bone formation within 1-2 weeks after injection in mice. Bone formation induced by BMP-2 in ALDHlo cells showed significantly more bone mineral content compared to that in ALDHbr cells. BMP-2 induces bone formation in heterogeneous osteosarcoma cells and BMP-2 may have a promising therapeutic role for treating human osteosarcoma by inducing differentiation along an osteogenic pathway.
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View References

1. 

Ottaviani G and Jaffe N: The epidemiology of osteosarcoma. Cancer Treat Res. 152:3–13. 2009. View Article : Google Scholar

2. 

Gorlick R and Khanna C: Osteosarcoma. J Bone Miner Res. 25:683–691. 2010. View Article : Google Scholar

3. 

Shimo T, Matsumura S, Ibaragi S, et al: Specific inhibitor of MEK-mediated cross-talk between ERK and p38 MAPK during differentiation of human osteosarcoma cells. J Cell Commun Signal. 1:103–111. 2007. View Article : Google Scholar : PubMed/NCBI

4. 

Luk F, Yu Y, Dong HT, Walsh WR and Yang JL: New gene groups associated with dissimilar osteoblastic differentiation are linked to osteosarcomagenesis. Cancer Genomics Proteomics. 8:65–75. 2011.PubMed/NCBI

5. 

Hruska KA, Mathew S and Saab G: Bone morphogenetic proteins in vascular calcification. Circ Res. 97:105–114. 2005. View Article : Google Scholar : PubMed/NCBI

6. 

Barboza E, Caula A and Machado F: Potential of recombinant human bone morphogenetic protein-2 in bone regeneration. Implant Dent. 8:360–367. 1999. View Article : Google Scholar : PubMed/NCBI

7. 

Hay E, Lemonnier J, Fromigue O, Guenou H and Marie PJ: Bone morphogenetic protein receptor IB signaling mediates apoptosis independently of differentiation in osteoblastic cells. J Biol Chem. 279:1650–1658. 2004. View Article : Google Scholar : PubMed/NCBI

8. 

Wrana JL: Regulation of Smad activity. Cell. 100:189–192. 2000. View Article : Google Scholar

9. 

Moustakas A and Heldin CH: Non-Smad TGF-beta signals. J Cell Sci. 118:3573–3584. 2005. View Article : Google Scholar : PubMed/NCBI

10. 

Guicheux J, Lemonnier J, Ghayor C, Suzuki A, Palmer G and Caverzasio J: Activation of p38 mitogen-activated protein kinase and c-Jun-NH2-terminal kinase by BMP-2 and their implication in the stimulation of osteoblastic cell differentiation. J Bone Miner Res. 18:2060–2068. 2003. View Article : Google Scholar : PubMed/NCBI

11. 

Arnold SF, Tims E and McGrath BE: Identification of bone morphogenetic proteins and their receptors in human breast cancer cell lines: importance of BMP2. Cytokine. 11:1031–1037. 1999. View Article : Google Scholar : PubMed/NCBI

12. 

Hardwick JC, Van Den Brink GR, Bleuming SA, et al: Bone morphogenetic protein 2 is expressed by and acts upon, mature epithelial cells in the colon. Gastroenterology. 126:111–121. 2004. View Article : Google Scholar : PubMed/NCBI

13. 

Kleeff J, Maruyama H, Ishiwata T, et al: Bone morphogenetic protein 2 exerts diverse effects on cell growth in vitro and is expressed in human pancreatic cancer in vivo. Gastroenterology. 116:1202–1216. 1999. View Article : Google Scholar : PubMed/NCBI

14. 

Soares AF, Xavier RL, da Costa Miguel MC, de Souza LB and Pinto LP: Bone morphogenetic protein-2/4 and bone morphogenetic protein receptor type IA expression in metastatic and nonmetastatic oral squamous cell carcinoma. Am J Otolaryngol. 31:266–271. 2010. View Article : Google Scholar : PubMed/NCBI

15. 

Kim IY, Lee DH, Lee DK, et al: Decreased expression of bone morphogenetic protein (BMP) receptor type II correlates with insensitivity to BMP-6 in human renal cell carcinoma cells. Clin Cancer Res. 9:6046–6051. 2003.PubMed/NCBI

16. 

Katoh M and Terada M: Overexpression of bone morphogenic protein (BMP)-4 mRNA in gastric cancer cell lines of poorly differentiated type. J Gastroenterol. 31:137–139. 1996. View Article : Google Scholar : PubMed/NCBI

17. 

Langenfeld EM, Calvano SE, Abou-Nukta F, Lowry SF, Amenta P and Langenfeld J: The mature bone morphogenetic protein-2 is aberrantly expressed in non-small cell lung carcinomas and stimulates tumor growth of A549 cells. Carcinogenesis. 24:1445–1454. 2003. View Article : Google Scholar : PubMed/NCBI

18. 

Langenfeld EM, Kong Y and Langenfeld J: Bone morphogenetic protein 2 stimulation of tumor growth involves the activation of Smad-1/5. Oncogene. 25:685–692. 2006. View Article : Google Scholar : PubMed/NCBI

19. 

Ide H, Yoshida T, Matsumoto N, et al: Growth regulation of human prostate cancer cells by bone morphogenetic protein-2. Cancer Res. 57:5022–5027. 1997.PubMed/NCBI

20. 

Beck SE, Jung BH, Fiorino A, et al: Bone morphogenetic protein signaling and growth suppression in colon cancer. Am J Physiol Gastrointest Liver Physiol. 291:G135–G145. 2006. View Article : Google Scholar : PubMed/NCBI

21. 

Brubaker KD, Corey E, Brown LG and Vessella RL: Bone morphogenetic protein signaling in prostate cancer cell lines. J Cell Biochem. 91:151–160. 2004. View Article : Google Scholar : PubMed/NCBI

22. 

Ghosh-Choudhury N, Woodruff K, Qi W, Celeste A, Abboud SL and Ghosh Choudhury G: Bone morphogenetic protein-2 blocks MDA MB 231 human breast cancer cell proliferation by inhibiting cyclin-dependent kinase-mediated retinoblastoma protein phosphorylation. Biochem Biophys Res Commun. 272:705–711. 2000. View Article : Google Scholar

23. 

Kawamura C, Kizaki M, Yamato K, et al: Bone morphogenetic protein-2 induces apoptosis in human myeloma cells with modulation of STAT3. Blood. 96:2005–2011. 2000.PubMed/NCBI

24. 

Wen XZ, Miyake S, Akiyama Y and Yuasa Y: BMP-2 modulates the proliferation and differentiation of normal and cancerous gastric cells. Biochem Biophys Res Commun. 316:100–106. 2004. View Article : Google Scholar : PubMed/NCBI

25. 

Wang L, Park P, Zhang H, et al: BMP-2 inhibits the tumorigenicity of cancer stem cells in human osteosarcoma OS99-1 cell line. Cancer Biol Ther. 11:457–463. 2011. View Article : Google Scholar : PubMed/NCBI

26. 

Wang L, Park P, Zhang H, et al: BMP-2 inhibits tumor growth of human renal cell carcinoma and induces bone formation. Int J Cancer. 131:1941–1950. 2012. View Article : Google Scholar

27. 

Gillette JM, Gibbs CP and Nielsen-Preiss SM: Establishment and characterization of OS 99-1, a cell line derived from a highly aggressive primary human osteosarcoma. In Vitro Cell Dev Biol Anim. 44:87–95. 2008. View Article : Google Scholar : PubMed/NCBI

28. 

Wang L, Park P, Zhang H, La Marca F and Lin CY: Prospective identification of tumorigenic osteosarcoma cancer stem cells in OS99-1 cells based on high aldehyde dehydrogenase activity. Int J Cancer. 128:294–303. 2011. View Article : Google Scholar : PubMed/NCBI

29. 

Eguchi Y, Wakitani S, Imai Y, et al: Antitumor necrotic factor agent promotes BMP-2-induced ectopic bone formation. J Bone Miner Metab. 28:157–164. 2010. View Article : Google Scholar : PubMed/NCBI

30. 

Luo X, Chen J, Song WX, et al: Osteogenic BMPs promote tumor growth of human osteosarcomas that harbor differentiation defects. Lab Invest. 88:1264–1277. 2008. View Article : Google Scholar : PubMed/NCBI

31. 

Le Page C, Puiffe ML, Meunier L, et al: BMP-2 signaling in ovarian cancer and its association with poor prognosis. J Ovarian Res. 2:42009.PubMed/NCBI

32. 

Kuo PL, Hsu YL, Chang CH and Chang JK: Osthole-mediated cell differentiation through bone morphogenetic protein-2/p38 and extracellular signal-regulated kinase 1/2 pathway in human osteoblast cells. J Pharmacol Exp Ther. 314:1290–1299. 2005. View Article : Google Scholar : PubMed/NCBI

33. 

Mirabello L, Troisi RJ and Savage SA: International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons. Int J Cancer. 125:229–234. 2009. View Article : Google Scholar : PubMed/NCBI

34. 

Tang N, Song WX, Luo J, Haydon RC and He TC: Osteosarcoma development and stem cell differentiation. Clin Orthop Relat Res. 466:2114–2130. 2008. View Article : Google Scholar : PubMed/NCBI

35. 

Wozney JM, Rosen V, Celeste AJ, et al: Novel regulators of bone formation: molecular clones and activities. Science. 242:1528–1534. 1988. View Article : Google Scholar : PubMed/NCBI

36. 

Katsuno Y, Hanyu A, Kanda H, et al: Bone morphogenetic protein signaling enhances invasion and bone metastasis of breast cancer cells through Smad pathway. Oncogene. 27:6322–6333. 2008. View Article : Google Scholar : PubMed/NCBI

37. 

Piccirillo SG, Reynolds BA, Zanetti N, et al: Bone morphogenetic proteins inhibit the tumorigenic potential of human brain tumour-initiating cells. Nature. 444:761–765. 2006. View Article : Google Scholar : PubMed/NCBI

38. 

Nozaki K, Kadosawa T, Nishimura R, Mochizuki M, Takahashi K and Sasaki N: 1,25-Dihydroxyvitamin D3, recombinant human transforming growth factor-beta 1 and recombinant human bone morphogenetic protein-2 induce in vitro differentiation of canine osteosarcoma cells. J Vet Med Sci. 61:649–656. 1999. View Article : Google Scholar

39. 

Orui H, Imaizumi S, Ogino T and Motoyama T: Effects of bone morphogenetic protein-2 on human tumor cell growth and differentiation: a preliminary report. J Orthop Sci. 5:600–604. 2000. View Article : Google Scholar : PubMed/NCBI

40. 

Ma Y, Ma L, Guo Q and Zhang S: Expression of bone morphogenetic protein-2 and its receptors in epithelial ovarian cancer and their influence on the prognosis of ovarian cancer patients. J Exp Clin Cancer Res. 29:852010. View Article : Google Scholar : PubMed/NCBI

41. 

Hall AK and Miller RH: Emerging roles for bone morphogenetic proteins in central nervous system glial biology. J Neurosci Res. 76:1–8. 2004. View Article : Google Scholar : PubMed/NCBI

42. 

Lee J, Son MJ, Woolard K, et al: Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells. Cancer Cell. 13:69–80. 2008. View Article : Google Scholar

43. 

Yamate J, Kotera T, Kuwamura M and Kotani T: Potential osteogenic differentiation of cisplatin-resistant rat malignant fibrous histiocytoma-derived cell lines. Exp Toxicol Pathol. 58:299–309. 2007. View Article : Google Scholar : PubMed/NCBI

44. 

Reddi AH: Bone morphogenetic proteins: from basic science to clinical applications. J Bone Joint Surg Am. 83-A(Suppl 1): S1–S6. 2001.PubMed/NCBI

45. 

Ulsamer A, Ortuno MJ, Ruiz S, et al: BMP-2 induces Osterix expression through up-regulation of Dlx5 and its phosphorylation by p38. J Biol Chem. 283:3816–3826. 2008. View Article : Google Scholar : PubMed/NCBI

46. 

Nakashima K, Zhou X, Kunkel G, et al: The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell. 108:17–29. 2002. View Article : Google Scholar : PubMed/NCBI

47. 

zur Nieden NI, Kempka G and Ahr HJ: In vitro differentiation of embryonic stem cells into mineralized osteoblasts. Differentiation. 71:18–27. 2003.PubMed/NCBI

48. 

Nohe A, Keating E, Knaus P and Petersen NO: Signal transduction of bone morphogenetic protein receptors. Cell Signal. 16:291–299. 2004. View Article : Google Scholar : PubMed/NCBI

49. 

Fong S, Chan MK, Fong A, Bowers WJ and Kelly KJ: Viral vector-induced expression of bone morphogenetic protein 2 produces inhibition of tumor growth and bone differentiation of stem cells. Cancer Gene Ther. 17:80–85. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Park P, La Marca F, Than K, Rahman S and Lin C: Bone formation induced by BMP-2 in human osteosarcoma cells . Int J Oncol 43: 1095-1102, 2013.
APA
Wang, L., Park, P., La Marca, F., Than, K., Rahman, S., & Lin, C. (2013). Bone formation induced by BMP-2 in human osteosarcoma cells . International Journal of Oncology, 43, 1095-1102. https://doi.org/10.3892/ijo.2013.2030
MLA
Wang, L., Park, P., La Marca, F., Than, K., Rahman, S., Lin, C."Bone formation induced by BMP-2 in human osteosarcoma cells ". International Journal of Oncology 43.4 (2013): 1095-1102.
Chicago
Wang, L., Park, P., La Marca, F., Than, K., Rahman, S., Lin, C."Bone formation induced by BMP-2 in human osteosarcoma cells ". International Journal of Oncology 43, no. 4 (2013): 1095-1102. https://doi.org/10.3892/ijo.2013.2030
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Park P, La Marca F, Than K, Rahman S and Lin C: Bone formation induced by BMP-2 in human osteosarcoma cells . Int J Oncol 43: 1095-1102, 2013.
APA
Wang, L., Park, P., La Marca, F., Than, K., Rahman, S., & Lin, C. (2013). Bone formation induced by BMP-2 in human osteosarcoma cells . International Journal of Oncology, 43, 1095-1102. https://doi.org/10.3892/ijo.2013.2030
MLA
Wang, L., Park, P., La Marca, F., Than, K., Rahman, S., Lin, C."Bone formation induced by BMP-2 in human osteosarcoma cells ". International Journal of Oncology 43.4 (2013): 1095-1102.
Chicago
Wang, L., Park, P., La Marca, F., Than, K., Rahman, S., Lin, C."Bone formation induced by BMP-2 in human osteosarcoma cells ". International Journal of Oncology 43, no. 4 (2013): 1095-1102. https://doi.org/10.3892/ijo.2013.2030
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