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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 2013 Volume 43 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

MiR-15a is underexpressed and inhibits the cell cycle by targeting CCNE1 in breast cancer

  • Authors:
    • Qifeng Luo
    • Xiaoyu Li
    • Jia Li
    • Xiangjie Kong
    • Junfeng Zhang
    • Lei Chen
    • Yixiang Huang
    • Lin Fang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China, Department of Microbiology and Genetic Institute, Paris-Sud 11 University, Paris, France
  • Pages: 1212-1218
    |
    Published online on: July 23, 2013
       https://doi.org/10.3892/ijo.2013.2034
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Abstract

MicroRNAs (miRNAs) are a small class of non-coding RNAs that are widely dysregulated in various cancers. They act as either oncogenes or tumor suppressor genes in human cancer. The purpose of this study was to examine the expression levels of miR-15a in human breast cancer and its potential role in disease pathogenesis. The expression levels of miR-15a were measured by quantitative polymerase chain reaction (qPCR) in 40 breast cancer specimens and adjacent normal breast tissues. MTT assays, colony formation assays, transwell chamber migration assays, cell cycle and apoptosis assays were used to explore the potential func­tion of miR-15a in MDA-MB-231 human breast cancer cells. Luciferase reporter assays were performed to validate the regulation of a putative target of miR-15a, in corroboration with qPCR and western blot assays. We found that the expression of miR-15a was lower in breast cancer specimens compared with adjacent normal tissues (p<0.05). Overexpression of miR-15a inhibited cellular growth, suppressed migration and arrested cells at the G1 phase, but did not promote cellular apoptosis. Luciferase assays indicated that miR-15a can bind with its putative target site in the 3'-untranslated region (3'-UTR) of CCNE1, suggesting that CCNE1 is a direct target of miR-15a. qPCR and western blot analysis indicated that the overexpression of miR-15a results in the downregulation of CCNE1 at the mRNA and protein levels. Taken together, the upregulation of miR-15a expression causes cellular growth inhibition, suppression of migration and G1 phase arrest by targeting CCNE1. These findings suggest that miR-15a may act as a tumor suppressor gene in breast cancer and that, in the future, it could be used as a therapeutic target for the treatment of breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Luo Q, Li X, Li J, Kong X, Zhang J, Chen L, Huang Y and Fang L: MiR-15a is underexpressed and inhibits the cell cycle by targeting CCNE1 in breast cancer. Int J Oncol 43: 1212-1218, 2013.
APA
Luo, Q., Li, X., Li, J., Kong, X., Zhang, J., Chen, L. ... Fang, L. (2013). MiR-15a is underexpressed and inhibits the cell cycle by targeting CCNE1 in breast cancer. International Journal of Oncology, 43, 1212-1218. https://doi.org/10.3892/ijo.2013.2034
MLA
Luo, Q., Li, X., Li, J., Kong, X., Zhang, J., Chen, L., Huang, Y., Fang, L."MiR-15a is underexpressed and inhibits the cell cycle by targeting CCNE1 in breast cancer". International Journal of Oncology 43.4 (2013): 1212-1218.
Chicago
Luo, Q., Li, X., Li, J., Kong, X., Zhang, J., Chen, L., Huang, Y., Fang, L."MiR-15a is underexpressed and inhibits the cell cycle by targeting CCNE1 in breast cancer". International Journal of Oncology 43, no. 4 (2013): 1212-1218. https://doi.org/10.3892/ijo.2013.2034
Copy and paste a formatted citation
x
Spandidos Publications style
Luo Q, Li X, Li J, Kong X, Zhang J, Chen L, Huang Y and Fang L: MiR-15a is underexpressed and inhibits the cell cycle by targeting CCNE1 in breast cancer. Int J Oncol 43: 1212-1218, 2013.
APA
Luo, Q., Li, X., Li, J., Kong, X., Zhang, J., Chen, L. ... Fang, L. (2013). MiR-15a is underexpressed and inhibits the cell cycle by targeting CCNE1 in breast cancer. International Journal of Oncology, 43, 1212-1218. https://doi.org/10.3892/ijo.2013.2034
MLA
Luo, Q., Li, X., Li, J., Kong, X., Zhang, J., Chen, L., Huang, Y., Fang, L."MiR-15a is underexpressed and inhibits the cell cycle by targeting CCNE1 in breast cancer". International Journal of Oncology 43.4 (2013): 1212-1218.
Chicago
Luo, Q., Li, X., Li, J., Kong, X., Zhang, J., Chen, L., Huang, Y., Fang, L."MiR-15a is underexpressed and inhibits the cell cycle by targeting CCNE1 in breast cancer". International Journal of Oncology 43, no. 4 (2013): 1212-1218. https://doi.org/10.3892/ijo.2013.2034
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