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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 2013 Volume 43 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

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Article

Therapeutic potential of human adipose stem cells in a cancer stem cell-like gastric cancer cell model

  • Authors:
    • Guangming Liu
    • Michael Neumeister
    • Joel Reichensperger
    • Russell D. Yang
  • View Affiliations / Copyright

    Affiliations: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Southern Illinois University School of Medicine, Springfield, IL 62794, USA, Department of Plastic Surgery, Southern Illinois University School of Medicine, Springfield, IL 62794, USA
  • Pages: 1301-1309
    |
    Published online on: July 25, 2013
       https://doi.org/10.3892/ijo.2013.2039
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Abstract

Cancer stem cells (CSCs) or circulating tumor cells play an important role in tumor initiation, invasion, metastasis and resistance to anticancer therapies. Therapies that target gastric tumor CSCs have potential clinical application for preventing malignant gastric tumor progression and metastasis. We isolated CD44+ gastric cancer cells from the gastric cancer cell line AGS and Hs746T cells and maintained the cells in a novel stem cell culture. The cells were kept in an undifferentiated proliferative state and we characterized their cancer stem cell properties and chemotherapy-resistance behavior. The CD44+ cancer cells were also co-cultured with human adipose stem cells (ADSCs) to determine the chemotherapy-promotion effects of the adipose cells on the CD44+ cancer cells. The CD44+ gastric cancer cell model is a non-adhesion, 3-dimensional, spheroid phenotype. The non-adherent CD44+ cells have cancer stem cell properties and are highly chemo-resistant. However, these cells regained chemo-sensitivity when re-attached to an extracellular matrix-coated attachment surface. The human adipose stem cells significantly promoted the chemo-sensitivity of the non-adherent CD44+ gastric cancer cells. Integrin α2/β2 and the Wnt signaling pathways are involved in the mechanisms. We concluded that the in vitro non-adherent CD44+ gastric cancer cell model resembles the circulating gastric tumor cells in vivo. Introduction of an appropriate attachment surface significantly promotes chemo-sensitivity of the non-adherent CD44+ gastric cancer cells. The human adipose stem cells function as a ‘living vehicle surface’ for such a purpose in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Liu G, Neumeister M, Reichensperger J and Yang RD: Therapeutic potential of human adipose stem cells in a cancer stem cell-like gastric cancer cell model. Int J Oncol 43: 1301-1309, 2013.
APA
Liu, G., Neumeister, M., Reichensperger, J., & Yang, R.D. (2013). Therapeutic potential of human adipose stem cells in a cancer stem cell-like gastric cancer cell model. International Journal of Oncology, 43, 1301-1309. https://doi.org/10.3892/ijo.2013.2039
MLA
Liu, G., Neumeister, M., Reichensperger, J., Yang, R. D."Therapeutic potential of human adipose stem cells in a cancer stem cell-like gastric cancer cell model". International Journal of Oncology 43.4 (2013): 1301-1309.
Chicago
Liu, G., Neumeister, M., Reichensperger, J., Yang, R. D."Therapeutic potential of human adipose stem cells in a cancer stem cell-like gastric cancer cell model". International Journal of Oncology 43, no. 4 (2013): 1301-1309. https://doi.org/10.3892/ijo.2013.2039
Copy and paste a formatted citation
x
Spandidos Publications style
Liu G, Neumeister M, Reichensperger J and Yang RD: Therapeutic potential of human adipose stem cells in a cancer stem cell-like gastric cancer cell model. Int J Oncol 43: 1301-1309, 2013.
APA
Liu, G., Neumeister, M., Reichensperger, J., & Yang, R.D. (2013). Therapeutic potential of human adipose stem cells in a cancer stem cell-like gastric cancer cell model. International Journal of Oncology, 43, 1301-1309. https://doi.org/10.3892/ijo.2013.2039
MLA
Liu, G., Neumeister, M., Reichensperger, J., Yang, R. D."Therapeutic potential of human adipose stem cells in a cancer stem cell-like gastric cancer cell model". International Journal of Oncology 43.4 (2013): 1301-1309.
Chicago
Liu, G., Neumeister, M., Reichensperger, J., Yang, R. D."Therapeutic potential of human adipose stem cells in a cancer stem cell-like gastric cancer cell model". International Journal of Oncology 43, no. 4 (2013): 1301-1309. https://doi.org/10.3892/ijo.2013.2039
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