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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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December 2013 Volume 43 Issue 6

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Aberrant expression of the PRAC gene in prostate cancer

  • Authors:
    • Govinda Lenka
    • Wen-Hui Weng
    • Cheng-Keng Chuang
    • Kwai-Fong Ng
    • See-Tong Pang
  • View Affiliations / Copyright

    Affiliations: Department of Chemical Engineering and Biotechnology, Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei, Taiwan, R.O.C., Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, LinKou, School of Medicine, Chang Gung University, Tao-Yuan, Taiwan, R.O.C., Department of Pathology, Chang Gung Memorial Hospital, LinKou, School of Medicine, Chang Gung University, Tao-Yuan, Taiwan, R.O.C.
  • Pages: 1960-1966
    |
    Published online on: October 2, 2013
       https://doi.org/10.3892/ijo.2013.2117
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Abstract

Identification of aberrant expression patterns of genes in prostate cancer (PCa) is a key step towards the development of effective therapies. Prostate-specific antigen (PSA) levels are commonly measured for the early detection of PCa, but which itself is still not an ideal biomarker. We analysed the expression patterns of prostate cancer susceptibility candidate (PRAC) in prostate cancer. The PRAC gene is known to be commonly expressed in prostate tissue, rectum and colon. To provide clear insights into the expression patterns of PRAC in PCa, we examined the gene expression by quantitative real-time PCR (qRT-PCR), western blot analysis and immunohistochemistry (IHC). The results showed that PRAC expression levels in androgen‑insensitive cells (DU145 and PC3) are lower than those in androgen-sensitive cell lines (LNCaP, LNCaP-R and CW22R). However, treatment of the LNCaP cell line with androgen and anti-androgen demonstrated that PRAC is expressed in an androgen-independent manner. Further, PRAC expression was restored upon treatment of DU145 and PC3 cells with the methyltransferase inhibitor, 5-aza-2'-deoxycytidine (5-aza-CdR), which indicates the effect of methylation in the control of PRAC expression. In addition, IHC analysis revealed a significantly decreased immunoreactivity of PRAC protein in PCa tissues compared to benign prostatic hyperplasia (BPH) (p<0.0001). Thus, our findings suggest that the pathogenesis of PCa may be due to the expression levels of PRAC protein, and this protein can serve as a potential biomarker for the management of PCa.
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Copy and paste a formatted citation
Spandidos Publications style
Lenka G, Weng W, Chuang C, Ng K and Pang S: Aberrant expression of the PRAC gene in prostate cancer. Int J Oncol 43: 1960-1966, 2013.
APA
Lenka, G., Weng, W., Chuang, C., Ng, K., & Pang, S. (2013). Aberrant expression of the PRAC gene in prostate cancer. International Journal of Oncology, 43, 1960-1966. https://doi.org/10.3892/ijo.2013.2117
MLA
Lenka, G., Weng, W., Chuang, C., Ng, K., Pang, S."Aberrant expression of the PRAC gene in prostate cancer". International Journal of Oncology 43.6 (2013): 1960-1966.
Chicago
Lenka, G., Weng, W., Chuang, C., Ng, K., Pang, S."Aberrant expression of the PRAC gene in prostate cancer". International Journal of Oncology 43, no. 6 (2013): 1960-1966. https://doi.org/10.3892/ijo.2013.2117
Copy and paste a formatted citation
x
Spandidos Publications style
Lenka G, Weng W, Chuang C, Ng K and Pang S: Aberrant expression of the PRAC gene in prostate cancer. Int J Oncol 43: 1960-1966, 2013.
APA
Lenka, G., Weng, W., Chuang, C., Ng, K., & Pang, S. (2013). Aberrant expression of the PRAC gene in prostate cancer. International Journal of Oncology, 43, 1960-1966. https://doi.org/10.3892/ijo.2013.2117
MLA
Lenka, G., Weng, W., Chuang, C., Ng, K., Pang, S."Aberrant expression of the PRAC gene in prostate cancer". International Journal of Oncology 43.6 (2013): 1960-1966.
Chicago
Lenka, G., Weng, W., Chuang, C., Ng, K., Pang, S."Aberrant expression of the PRAC gene in prostate cancer". International Journal of Oncology 43, no. 6 (2013): 1960-1966. https://doi.org/10.3892/ijo.2013.2117
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