Establishment of a 5-fluorouracil-resistant triple-negative breast cancer cell line

Introduction

Triple-negative breast cancers (TNBCs) are defined as tumors that lack expression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) (1). TNBC patients account for 11–23% of all breast cancers (2–4). TNBCs follow a more aggressive clinical course than other forms, such as luminal A and luminal B, and have a poor prognosis (4). They also have no indications for hormonal therapy or anti-HER2 therapy. Therefore, treatment of TNBC patients is restricted to cytotoxic drugs such as 5-fluorouracil (5-FU), vinorelbine (VNB), paclitaxel (PTX), doxorubicin (DOX), and gemcitabine (GEM) (5). However, TNBCs acquire resistance to cytotoxic drugs after a series of treatments (6). The development of resistance to cytotoxic drugs appears to have become a major clinical problem in the chemotherapy of TNBCs.

Drug efflux mechanisms are the most well-studied mechanisms of drug resistance. The ABC family proteins, which include multidrug resistance protein 1 (MDR1) and breast cancer resistance protein (BCRP), are target molecules to overcome drug resistance (6–8). However, the combination of these protein inhibitors and cytotoxic drugs failed to show an improved outcome over cytotoxic drugs alone (9,10).

Fluoropyrimidine anticancer drugs, as represented by 5-FU and capecitabine, have been used to treat various cancers and accepted worldwide as first-line anticancer drugs for breast cancers (11). The mechanisms of resistance to 5-FU, namely, enhanced activities of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPYD), are well known to endow cancer cells with resistance to 5-FU in vitro and in clinical studies (12–15). Actually, 5-FU is used in combination with 5-chloro-2,4-dihydroxypyridine, which is a DPYD inhibitor. However, the effects of this combination are insufficient. The elucidation of other mechanisms of resistance to 5-FU are thus anticipated. Investigations of other mechanisms of 5-FU resistance may lead to the development of novel effective anticancer chemotherapies for 5-FU-resistant patients. To elucidate mechanisms of drug resistance, the establishment of drug-resistant cancer cell lines should be one of the most useful approaches for developing model systems (16–18). However, 5-FU-resistant TNBC cell lines have not been previously reported, although there have been some 5-FU-resistant lines of other forms of breast cancer or other tumors (11).

In this study, we established a 5-FU-resistant cell line, MDA-MB-231/5-FU, from the human TNBC cancer cell line MDA-MB-231, by repeated exposure of cells to stepwise increases in the concentration of 5-FU. Then, we applied a proteomic approach and the quantification of protein expression to compare proteins between MDA-MB-231/5-FU and MDA-MB-231 and identify those with differential expression. MDA-MB-231/5-FU may be a useful tool for identifying new mechanisms of drug resistance and new drug targets in TNBCs.

Materials and methods

Chemicals and antibodies

5-FU, DOX and VNB were purchased from Kyowa Hakko (Tokyo, Japan), CDDP from Pfizer (New York, NY, USA), PTX from Bristol-Myers (New York, NY, USA), and GEM from Eli Lilly (Indianapolis, IN, USA). MDR1, p53 and phospho-p53 (Ser15) were purchased from Cell Signaling Technology (Beverly, MA, USA), DPYD and TS from GeneTex (San Antonio, TX, USA), BCRP from Abcam (Cambridge, UK), and β-actin from Sigma (St. Louis, MO, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from GE Healthcare (Little Chalfont, Bucks, UK).

Cell lines and culture conditions

The human breast carcinoma cell line MDA-MB-231 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Wako, Osaka, Japan) with 10% fetal bovine serum (FBS) (Equitech-Bio, Kerrville, TX, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco, Grand Island, NE, USA) at 37°C under 5% CO2 and 20% O2 in a humidified chamber.

Establishment of 5-FU-resistant cell line

5-FU-resistant cells were established from MDA-MB-231 by exposure to increasing concentrations of 5-FU. MDA-MB-231 were exposed to an initial 5-FU concentration of 3.84 μmol/l in DMEM plus 10% FBS. The drug concentration was then increased 1.25 times at each step of resistance, from 3.84 μmol/l up to 23.0 μmol/l. Cells were cultured for at least four weeks at each step, with medium exchange every three days. Chemotherapeutic drugs were eliminated from the 5-FU-resistant MDA-MB-231 (MDA-MB-231/5-FU) for 15 days before each experiment.

Cell proliferation assays

Cell proliferation was examined using a Cell Counting Kit-8 (Dojindo Laboratories, Kumamoto, Japan) in accordance with the manufacturer’s instructions. Briefly, a suspension of MDA-MB-231 or MDA-MB-231/5-FU (1.5×103 cells/well) in 100 μl of DMEM with 10% FBS was seeded to 96-well plates, and supplemented with 5-FU, DOX, CDDP, VNB, PTX and GEM. After incubation for 72 h, Cell Counting Kit-8 reagent was added to each well. After incubation for 90 min, the cell viability was measured as absorbance at 450 nm using a microplate reader (Perkin-Elmer, Waltham, MA, USA). Analyses of all samples were performed in triplicate. The percentage of cell viability was determined as the ratio of absorbance of the sample versus that without 5-FU as a control. The IC50 of a chemotherapeutic drug was determined as the concentration at which 50% inhibition of cell growth was shown compared with the control cell growth.

Protein extraction

Cells were washed with PBS and lysed in lysis buffer consisting of 50 mM HEPES, pH 8.0, 150 mM NaCl, 5 mM EDTA, 1% NP-40, 10% glycerol, 100 mM NaF, 1 mM phenylmethylsulfonyl fluoride and protease inhibitor cocktail (Nacalai Tesque Inc., Kyoto, Japan). Lysates were separated by centrifugation, the supernatant was recovered, and protein concentrations were assayed using the bicinchoninic acid protein assay reagent (Thermo Fisher Scientific, Rockford, IL, USA).

iTRAQ sample labeling

TheiTRAQ analysis was performed in a double duplex manner. Protein lysates (170 μg) from MDA-MB-231 and MDA-MB-231/5-FU were digested with trypsin and labeled with 114 and 117 iTRAQ reagents according to standard procedures.

Protein identification and relative quantification

Proteomic analysis was performed on a DiNa-AI Nano LC System (KYA Technologies, Tokyo, Japan) coupled to a QSTAR Elite hybrid mass spectrometer (AB Sciex, Framingham, MA, USA) through a NanoSpray ion source (AB Sciex) as previously described (19). Briefly, mobile phase A was 98% water [2% acetonitrile (ACN), 0.1% formic acid], and mobile phase B was 70% ACN (0.1% formic acid, 30% water). The column effluent was introduced into the spray chamber through a tapered stainless steel emitter and directly electrosprayed into the QSTAR System ion trap mass spectrometer in the positive mode for nano-electrospray ionization-MS/MS analysis. Each sample was run for 150 min. Protein identification was performed using Analyst QS Software 2.0 (AB Sciex) in the positive-ion mode. Both sets of data were processed using ProteinPilot Software 2.0.1 with the Paragon™ search algorithm (AB Sciex). MS/MS data were searched against the NCBI database (RefSeq release 54 of July 2012 from the website ftp://ftp.hgc.jp/pub/mirror/ncbi/refseq/) using a Homosapiens taxonomy filter. The minimum threshold for protein identification was set at a protein score of 0.47, corresponding to a confidence level >66% and 1% false discovery rate.

Annotation analysis

GI accession numbers were uploaded into the DAVID 6.7 (Database for Annotation, Visualization, and Integrated Discovery) information tool. For Gene Ontology (GO) term analysis, we studied the ‘Biological Process’ categories using the GO FAT default settings. For functional annotation searches, we set the following parameters: ‘Biological Process’, threshold count 3, EASE 0.5; for functional annotation clusters, medium stringency. Enrichment values (GO terms), enrichment scores (annotation clusters), and statistical determinants (Fisher’s Exact P-values) are those calculated using DAVID 6.7 software.

Western blotting

The lysates for western blotting (20 μg of protein) were separated on sodium dodecyl sulfate-polyacrylamide gels under reducing conditions, followed by electrophoretic transfer to polyvinylidene difluoride membranes (Immobilon-P; Millipore, Billerica, MA, USA). After blocking, the membranes were probed with the appropriate primary antibodies. Membrane-bound primary antibodies were detected using secondary antibodies conjugated with HRP. The chemiluminescence was detected with LAS-4000 (GE Healthcare) using the enhanced chemiluminescence technique and quantified using Image Quant TL software (GE Healthcare).

Results

Establishment of 5-FU-resistant TNBC cell line

To explore the mechanisms of resistance to 5-FU, we established a 5-FU-resistant TNBC cell line. To achieve this, a human TNBC cell line, MDA-MB-231, was treated continuously with stepwise increases of the concentration of 5-FU every four weeks from 3.84 μmol/l to 23.0 μmol/l. Fig. 1 shows cell survival curves of MDA-MB-231 and 5-FU-resistant cells. The cells were treated with different concentrations of 5-FU for 72 h. The IC50 values of parent cells and 5-FU-resistant cells to 5-FU were 29.9±2.3 and 165.5±21.8 μmol/l, respectively (P<0.01). The new cells were thus successfully established as a 5-FU-resistant TNBC cell line: MDA-MB-231/5-FU.

Figure 1. Dose response curve of MDA-MB-231 and MDA-MB-231/5-FU to 5-FU. MTS assay was carried out after 72 h of exposure to 5-FU. Dose response curve plotted from the results of MTS assays performed four times. Both cell lines displayed dose-dependent inhibition of cell growth. The IC50 values to 5-FU for MDA-MB-231 and MDA-MB-231/5-FU were 29.9±2.3 and 165.5±21.8 μM, respectively (P<0.01). The MDA-MB-231/5-FU cells were thus more resistant to 5-FU. The results are expressed as the means ± standard error.

Cross-resistance profiles of MDA-MB-231/5-FU cells

MDA-MB-231/5-FU acquired resistance to 5-FU; its resistant index (RI) was 5.5. Generally, multiple drug resistance involves resistance to one drug accompanied by resistance to several other anticancer drugs (16). Therefore, we evaluated whether MDA-MB-231/5-FU acquired cross-resistance to other anticancer drugs used for TNBCs or with other mechanisms of action. The IC50 and RI are summarized in Table I. The IC50 values of parent cells to DOX, CDDP, VNB, PTX and GEM were 38.2±3.3 nmol/l, 2.0±0.3 μmol/l, 2.1±0.8 nmol/l, 1.1±0.7 nmol/l and 33.4±5.7 pmol/l, respectively. In contrast, the IC50 values of MDA-MB-231/5-FUto DOX, CDDP, VNB, PTX and GEM were 49.3±1.8 nmol/l, 1.4±0.2 μmol/l, 5.2±0.9 nmol/l, 9.5±2.0 nmol/l and 270.1±15.4 pmol/l, respectively. The RI of DOX, CDDP, VNB, PTX and GEM were 1.3, 0.7, 2.5, 8.4 and 8.1, respectively. MDA-MB-231/5-FU acquired cross-resistance to VNB, PTX and GEM. However, these cells were sensitive to DOX and CDDP.

Table I. Cross-resistance of MDA-MB-231/5-FU cells.

Table I.

Cross-resistance of MDA-MB-231/5-FU cells.

	5-FU		DOX		CDDP		VNB		PTX		GEM
Cell line	IC50 (μmol/l)	RI	IC50 (nmol/l)	RI	IC50 (μmol/l)	RI	IC50 (nmol/l)	RI	IC50 (nmol/l)	RI	IC50 (pmol/l)	RI
MDA-MB-231	29.9	5.5a	38.2	1.3	2.0	0.7	2.1	2.5b	1.1	8.4a	33.4	8.1a
MDA-MB-231/5-FU	165.5		49.3		1.4		5.2		9.5		270.1

{ label (or @symbol) needed for fn[@id='tfn1-ijo-43-06-1985'] } The resistance index (RI) was determined as the ratio of IC₅₀ between MDA-MB-231/5-FU and MDA-MB-231. The IC₅₀ of MDA-MB-231/5-FU to 5-FU, vinorelbine (VNB), paclitaxel (PTX) and gemcitabine (GEM) were significantly increased compared with those of parent cells. The results show that MDA-MB-231/5FU is resistant to multiple drugs.

a Significantly different from the parent cell line, P<0.01.

b Significantly different from the parent cell line, P<0.05.

Western blot analysis of proteins related to drug resistance

According to previous studies, the mechanisms of resistance to 5-FU involve increases in 5-FU-degrading enzyme DPYD and 5-FU-targeting enzyme TS (11–15,20). On the other hand, ABC family proteins, such as MDR1 and BCRP, are related to multiple drug resistance in breast cancer (6–8). To confirm the expression of proteins related to drug resistance, we examined MDR1, BCRP, DPYD and TS expression by western blot analysis. MDA-MB-231/5-FU showed increased levels of MDR1 and BCRP1 proteins compared with the parent cells (Fig. 2A). In contrast, there were no significant differences in DPYD and TS between the parent cells and MDA-MB-231/5-FU.

Figure 2. Western blot analysis of proteins related to drug resistance. After cells were harvested at 80% confluence, total proteins were extracted by the protocol described in Materials and methods. (A) MDA-MB-231/5-FU cells exhibited increased levels of MDR1 and BCRP proteins compared with MDAMB-231. β-actin was used as a loading control. (B) MDA-MB-231 and MDA-MB-231/5-FU were treated with 30 μM 5-FU (approximately the IC50 of parent cells). MDA-MB-231 cells exhibited increased levels of p53 and phospho-p53 proteins.

p53 plays a major role in cellular responses to DNA damage and other genomic aberrations (21). Activation of p53 can lead to cell cycle arrest, DNA repair, or apoptosis. Generally, phosphorylation of p53 is increased by DNA damage due to 5-FU (22–25). To evaluate the response to DNA damage, MDA-MB-231 and MDA-MB-231/5-FU were treated with 30 μM 5-FU for 6, 12 and 24 h. The phosphorylation level of p53 was increased by 5-FU in MDA-MB-231, but not in its 5-FU-resistant counterpart (Fig. 2B). These results suggested that DNA damage due to 5-FU was avoided by the over expression of ABC family proteins.

Quantitative differential proteomics in MDA-MB-231/5FU cells

To characterize MDA-MB-231/5-FU, we performed quantitative differential proteomic analysis of MDA-MB-231/5-FU cells and the parent cells based on the iTRAQ technique. As a result, 93 proteins with a change of expression of ≥1.2-fold were considered to be upregulated, whereas 85 proteins with a change <0.8-fold were downregulated. Table II shows the proteins for which there was a change of expression ≥1.2-fold that was significant at the level of P<0.05. To evaluate the functional differences between parent cells and MDA-MB-231/5-FU cells, we performed enrichment analysis (Fig. 3). The upregulated proteins (≥1.2-fold) were classified into the GO categories of ‘DNA recombination’, ‘cell cycle’, ‘complex assembly’, ‘cytoskeleton organization’, ‘transport’, ‘negative regulation of cell death’, ‘chromatin organization’, and ‘cell differentiation’. The enrichment scores for ‘DNA recombination’, ‘cell cycle’ and ‘complex assembly’ were 1.98, 1.95 and 1.81, respectively.

Figure 3. Functional annotation clusters (‘Biological Process’) in MDA-MB-231/5-FU upregulated proteins. Identification of proteins in MDA-MB-231/5-FU cells was performed by quantitative proteomics using stable isotope labeling, via iTRAQ. The most enriched clusters and characteristic examples of their Gene Ontology terms are shown, together with their enrichment scores. Bars show the count (number of included protein species). The line with the closed circles shows GO term enrichment score.

Table II. Identification of upregulated proteins in MDA-MB-231/5FU cells.

Table II.

Identification of upregulated proteins in MDA-MB-231/5FU cells.

Accession no.	Protein name	117/114	P-value
gi|4501881	Actin, α skeletal muscle	6.870
gi|62750354	Matrin-3 isoform a	3.242
gi|9257257	WD repeat-containing protein 1 isoform 1	2.333	0.007
gi|156523970	α-2-HS-glycoprotein preproprotein	2.216	0.000
gi|4506145	Trypsin-1 preproprotein	2.193	0.000
gi|62414289	Vimentin	2.014	0.000
gi|4503515	Eukaryotic translation initiation factor 3 subunit H	1.906
gi|5803013	Endoplasmic reticulum resident protein 29 isoform 1 precursor	1.905	0.037
gi|28373194	Proteasomal ubiquitin receptor ADRM1 precursor	1.893
gi|5031635	Cofilin-1	1.821	0.000
gi|4507879	Voltage-dependent anion-selective channel protein 1	1.781	0.013
gi|50053795	Eukaryotic translation initiation factor 4B	1.679	0.002
gi|167614506	Plastin-2	1.669	0.028
gi|4758516	Hepatoma-derived growth factor isoform a	1.663	0.015
gi|4758756	Nucleosome assembly protein 1-like 1	1.575	0.000
gi|112380628	Lysosome-associated membrane glycoprotein 1 precursor	1.567
gi|4503481	Elongation factor 1-γ	1.560	0.000
gi|23110935	Proteasome subunit α type-1 isoform 1	1.493	0.024
gi|25777713	S-phase kinase-associated protein 1 isoform b	1.490
gi|19743823	Integrin β-1 isoform 1A precursor	1.488	0.001
gi|4506671	60S acidic ribosomal protein P2	1.479	0.000
gi|5032057	Protein S100-A11	1.479	0.005
gi|4757768	Rho GDP-dissociation inhibitor 1 isoform a	1.454	0.003
gi|5901912	Calmodulin	1.448	0.001
gi|386642862	Threonine-tRNA ligase, cytoplasmic isoform 2	1.444	0.010
gi|4758484	Glutathione S-transferase ω-1 isoform 1	1.441	0.023
gi|4504251	Histone H2A type 2-A	1.429	0.021
gi|6031192	Phosphate carrier protein, mitochondrial isoform a precursor	1.427	0.024
gi|10863927	Peptidyl-prolyl cis-trans isomerase A	1.414	0.001
gi|73486658	Aspartate aminotransferase, mitochondrial precursor	1.396	0.019
gi|119395750	Keratin, type II cytoskeletal 1	1.388	0.004
gi|385298707	Hippocalcin-like protein 1	1.370	0.005
gi|50592994	Thioredoxin isoform 1	1.356	0.045
gi|4503471	Elongation factor 1-α 1	1.305	0.002
gi|24307939	T-complex protein 1 subunit ɛ	1.297	0.003
gi|4758950	Peptidyl-prolyl cis-trans isomerase B precursor	1.289	0.009
gi|38327039	Heat shock 70 kDa protein 4	1.286	0.003
gi|42544159	Heat shock protein 105 kDa	1.258	0.008
gi|98986464	Transmembrane emp24 domain-containing protein 10 precursor	1.242	0.002
gi|4758012	Clathrin heavy chain 1	1.221	0.011
gi|5453603	T-complex protein 1 subunit β isoform 1	1.215	0.022
gi|4506663	60S ribosomal protein L8	1.206	0.041
gi|5901922	Hsp90 co-chaperone Cdc37	1.204	0.045

[i] 117/114: Ratios between the two groups of 114, MDA-MB-231; and 117, MDA-MB-231/5-FU.

Discussion

In this study, a 5-FU-resistant TNBC cell line was established from the TNBC cell line MDA-MB-231 by continuous exposure to stepwise increases in the concentration of 5-FU. The IC50 of 5-FU for the 5-FU-resistant MDA-MB-231 was significantly increased compared with that for MDA-MB-231. Moreover, MDA-MB-231/5-FU acquired cross-resistance to VNB, PTX and GEM. To the best of our knowledge, this is the first study on the establishment of a 5-FU-resistant TNBC cell line. MDA-MB-231/5-FU should be useful to study the mechanisms underlying the 5-FU resistance of TNBCs.

Recent studies have reported several determinants of 5-FU resistance mechanisms (15,26,27). For instance, TS, a 5-FU-targeting enzyme; DPYD, a 5-FU-degrading enzyme; and OPRT, a 5-FUanabolic enzyme, play key roles in the 5-FU metabolism pathway. A previous study reported that the expression of DPYD and TS was enhanced in 5-FU-resistant cell lines (12). However, the expression of DPYD and TS was not enhanced in MDA-MB-231/5-FU. This suggests that the mechanisms of 5-FU resistance of MDA-MB-231/5-FU differ from those generally reported previously. Of note, MDA-MB-231/5-FU showed cross-resistance to other anticancer drugs, such as PTX, VNB and GEM. Likewise, it was reported that acquisition of 5-FU resistance led to the acquisition of cross-resistance to other anticancer drugs in gastric cancer cells (16). Multiple drug resistance describes a phenomenon whereby resistance to one drug is accompanied by resistance to other drugs whose structures and mechanisms of action may be completely different. Mechanisms of multiple drug resistance have been associated with increased drug efflux from cells, which is mediated by an energy-dependent mechanism (8).

The ABC family proteins, which include MDR1 and BCRP, play key roles in multiple drug resistance in breast cancer (6–8). Overexpression of MDR1 confers resistance to a variety of anticancer drugs, which are structurally and functionally unrelated, including vincristine, VNB, etoposide, PTX and many others. The expression of MDR1 and BCRP is increased in MDA-MB-231/5-FU. The overexpression of these proteins may thus be related to the partial contribution of drug efflux to multiple drug resistance in these newly established cells. To consider what kind of protein expression is enhanced other than that of ABC family proteins, we performed iTRAQ-based quantitative proteomics on MDA-MB-231/5-FU and the parent cells. The upregulated proteins were classified into the GO categories of ‘DNA recombination’, ‘cell cycle’, ‘complex assembly’, ‘transport’ and ‘negative regulation of cell death’. These results suggest that MDA-NB-231/5-FU cells were resistant to 5-FU by the enhancement of DNA recombination, regulation of the cell cycle, homologous recombination and anti-apoptotic functions. These categorized proteins can be related to mechanisms of drug resistance in MDA-MB-231/5-FU. S-phase kinase-associated protein 1 (Skp1), categorized as being involved in ‘DNA recombination’, exhibited a 1.49-fold increase in MDA-MB-231/5-FU compared with that in the parent cells. Skp1 is composed of the Skp, Cullin and F-box (SCF)-containing complex, which plays an important role in regulating the ubiquitination of specific protein substrates and regulators of cell cycle progression and development. Skp1 binds directly to F-box motifs found in F-box proteins, such as Skp2, FBW7 and β-transducin repeat-containing protein (28,29). SCF protein complex regulates Akt ubiquitination, glycolysis and tumorigenesis in breast cancer (30). MDA-MB-231/5-FU may thus show enhanced ubiquitination and cell cycle progression because of the overexpression of Skp1. Likewise, peptidyl-prolyl cis-trans isomerase A, originally identified as an intracellular receptor for cyclosporine A, exhibited a 1.41-fold increase in MDA-MB-231/5-FU compared with that in the parent cells. The immunosuppressive activity of cyclosporine A is thought to be mediated by the engagement of calcineurin by the cyclosporin A-peptidyl-prolyl cis-trans isomerase A complex, an observation supported by the finding that peptidylprolyl cis-trans isom-erase A-knockout mice are resistant to immunosuppression by cyclosporin A (31,32). Peptidyl-prolyl cis-trans isomerase A was shown to be upregulated in 5-FU-treated colorectal cancer cells (33). Moreover, the overexpression of peptidyl-prolyl cistrans isomerase A induced chemoresistance to GEM (34). In this study, overexpression of ABC family proteins was observed in MDA-MB-231/5-FU. However, we maintain that the acquisition of multidrug resistance was not only due to the increased expression of ABC family proteins. In accordance with the above findings, MDA-MB-231/5-FU should be useful to identify factors that contribute to chemoresistance in TNBCs.

Clinically, TNBC patients are treated with combination therapy of 5-FU, epirubicin and cyclophosphamide at the first-line approach. If these drugs have no effect on disease progression, PTX is applied as a second-line treatment and GEM, VNB, or other drugs as a third-line treatment. However, our 5-FU-resistant TNBC cell line acquired resistance to 5-FU, VNB, PTX and GEM. TNBCs are generally more aggressive than the standard level owing to drug resistance that developed via previous chemotherapy. This indicates that TNBC patients acquire resistance to 5-FU via the development of cross-resistance to VNB, PTX and GEM. Thus, the MDA-MB-231/5-FU established in this study should be useful for identifying new mechanisms of drug resistance and new drug targets.

Acknowledgements

The authors also wish to thank members of the Central Laboratory of Osaka City University Graduate School of Medicine, for providing technical support. This work was supported by JSPS KAKENHI Grant number 24650647 (to Katsuyuki Takahashi).

References