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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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December 2013 Volume 43 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

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Article

Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines

  • Authors:
    • Ja-Lok Ku
    • Sung-Chan Park
    • Kyung-Hee Kim
    • You-Kyung Jeon
    • Sung-Hee Kim
    • Young-Kyoung Shin
    • Dong-Young Noh
    • Seock-Ah Im
    • Yung-Jue Bang
    • Wonshik Han
    • Woo Ho Kim
    • Jae-Gahb Park
  • View Affiliations / Copyright

    Affiliations: Korean Cell Line Bank, Laboratory of Cell Biology, Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea, Department of Surgery, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea, Division of Hematology and Medical Oncology, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea, Department of Pathology, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea
  • Pages: 2073-2081
    |
    Published online on: October 18, 2013
       https://doi.org/10.3892/ijo.2013.2144
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Abstract

Permanently growing cell lines can be invaluable because of their usefulness in a variety of experimental situations. We report the characteristics of seven cell lines designated, SNU-306, SNU-334, SNU-1528, SNU-1553, SNU-1581, SNU-1958 and SNU-2372, which were established from three primary carcinomas, two pleural effusion, one pericardial effusion and one ascitic fluid samples obtained from seven Korean breast carcinoma patients. The histopathology of the primary tumors and their in vitro growth characteristics are described. DNA fingerprinting analysis and genetic alterations in the p53 and EGFR genes were conducted. The expression levels of the ER-α, PR, C-erbB2, E-cadherin, COX-2, MDR and MXR genes were investigated and sensitivity to anticancer drugs was screened. Growth was as adherent cells (four cell lines), floating aggregates (one cell line) and both (two cell lines). All lines were free of mycoplasma or bacteria and were proven unique by DNA fingerprinting analysis using 18 microsatellite markers. Estrogen receptor (ER) mRNA was highly expressed in five cell lines and low or undetectable in SNU-1958 and SNU-2372. Progesterone receptor (PR) mRNA was expressed only in the SNU-306. SNU-1958 and SNU-2372 were hormone receptor-negative and C-erbB2-negative (triple-negative). SNU-1528 had an in-frame deletion of 42 base pairs of p53 gene and showed over 20-fold resistance for taxol compared to the other cell lines. There were no mutation in the EGFR gene; COX-2 was expressed in four cell lines and MXR was expressed in two cell lines. These well-characterized seven breast cancer cell lines, which include two triple-negative cell lines, will be useful for the study of breast cancer biology.
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Copy and paste a formatted citation
Spandidos Publications style
Ku J, Park S, Kim K, Jeon Y, Kim S, Shin Y, Noh D, Im S, Bang Y, Han W, Han W, et al: Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines. Int J Oncol 43: 2073-2081, 2013.
APA
Ku, J., Park, S., Kim, K., Jeon, Y., Kim, S., Shin, Y. ... Park, J. (2013). Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines. International Journal of Oncology, 43, 2073-2081. https://doi.org/10.3892/ijo.2013.2144
MLA
Ku, J., Park, S., Kim, K., Jeon, Y., Kim, S., Shin, Y., Noh, D., Im, S., Bang, Y., Han, W., Kim, W. H., Park, J."Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines". International Journal of Oncology 43.6 (2013): 2073-2081.
Chicago
Ku, J., Park, S., Kim, K., Jeon, Y., Kim, S., Shin, Y., Noh, D., Im, S., Bang, Y., Han, W., Kim, W. H., Park, J."Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines". International Journal of Oncology 43, no. 6 (2013): 2073-2081. https://doi.org/10.3892/ijo.2013.2144
Copy and paste a formatted citation
x
Spandidos Publications style
Ku J, Park S, Kim K, Jeon Y, Kim S, Shin Y, Noh D, Im S, Bang Y, Han W, Han W, et al: Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines. Int J Oncol 43: 2073-2081, 2013.
APA
Ku, J., Park, S., Kim, K., Jeon, Y., Kim, S., Shin, Y. ... Park, J. (2013). Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines. International Journal of Oncology, 43, 2073-2081. https://doi.org/10.3892/ijo.2013.2144
MLA
Ku, J., Park, S., Kim, K., Jeon, Y., Kim, S., Shin, Y., Noh, D., Im, S., Bang, Y., Han, W., Kim, W. H., Park, J."Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines". International Journal of Oncology 43.6 (2013): 2073-2081.
Chicago
Ku, J., Park, S., Kim, K., Jeon, Y., Kim, S., Shin, Y., Noh, D., Im, S., Bang, Y., Han, W., Kim, W. H., Park, J."Establishment and characterization of seven human breast cancer cell lines including two triple-negative cell lines". International Journal of Oncology 43, no. 6 (2013): 2073-2081. https://doi.org/10.3892/ijo.2013.2144
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