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Article

Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells

  • Authors:
    • Xia Zhao
    • Atsushi Nogawa
    • Tsukasa Matsunaga
    • Tsutomu Takegami
    • Hideaki Nakagawa
    • Yasuhito Ishigaki
  • View Affiliations / Copyright

    Affiliations: Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R. China, Laboratory of Human Molecular Genetics, School of Pharmaceutical Sciences, Kanazawa University, Kakuma, Kanazawa 920-1192, Japan, Medical Research Institute, Kanazawa Medical University, Kahoku 920-0293, Japan
  • Pages: 222-228
    |
    Published online on: October 25, 2013
       https://doi.org/10.3892/ijo.2013.2149
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Abstract

The ubiquitin-proteasome system (UPS) is one of the most promising anticancer drug targets of the century. However, the involved molecular mechanisms are still unclear. The nonsense-mediated mRNA decay (NMD) pathway is a highly conserved pathway which degrades nonsense mutation‑containing mRNA selectively and efficiently. In this pathway, the SMG1-Upf1-eRF (SURF) complex binds to Upf2 on the exon junction complex and finally causes degradation of nonsense-containing mRNA. To reveal the relationship between the UPS and NMD pathways, we analyzed the effects of proteasome inhibitors on Upf1 and Upf2. The data showed that treatment with proteasome inhibitors caused the accumulation of the Upf1 and Upf2 proteins in A549 cells. In addition, we found that knockdown of SMG1 also caused the upregulation of Upf1 and Upf2 proteins, which was confirmed by different target sequences of siRNA. SMG1 and UPS appear to participate in different pathways of the degradation of Upf1 and Upf2, since simultaneous treatment with both of them caused additive effects. This study demonstrated the quantitative regulation of Upf1 and Upf2 proteins by UPS and SMG1.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao X, Nogawa A, Matsunaga T, Takegami T, Nakagawa H and Ishigaki Y: Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells. Int J Oncol 44: 222-228, 2014.
APA
Zhao, X., Nogawa, A., Matsunaga, T., Takegami, T., Nakagawa, H., & Ishigaki, Y. (2014). Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells. International Journal of Oncology, 44, 222-228. https://doi.org/10.3892/ijo.2013.2149
MLA
Zhao, X., Nogawa, A., Matsunaga, T., Takegami, T., Nakagawa, H., Ishigaki, Y."Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells". International Journal of Oncology 44.1 (2014): 222-228.
Chicago
Zhao, X., Nogawa, A., Matsunaga, T., Takegami, T., Nakagawa, H., Ishigaki, Y."Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells". International Journal of Oncology 44, no. 1 (2014): 222-228. https://doi.org/10.3892/ijo.2013.2149
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao X, Nogawa A, Matsunaga T, Takegami T, Nakagawa H and Ishigaki Y: Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells. Int J Oncol 44: 222-228, 2014.
APA
Zhao, X., Nogawa, A., Matsunaga, T., Takegami, T., Nakagawa, H., & Ishigaki, Y. (2014). Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells. International Journal of Oncology, 44, 222-228. https://doi.org/10.3892/ijo.2013.2149
MLA
Zhao, X., Nogawa, A., Matsunaga, T., Takegami, T., Nakagawa, H., Ishigaki, Y."Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells". International Journal of Oncology 44.1 (2014): 222-228.
Chicago
Zhao, X., Nogawa, A., Matsunaga, T., Takegami, T., Nakagawa, H., Ishigaki, Y."Proteasome inhibitors and knockdown of SMG1 cause accumulation of Upf1 and Upf2 in human cells". International Journal of Oncology 44, no. 1 (2014): 222-228. https://doi.org/10.3892/ijo.2013.2149
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